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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳焜裕 | |
| dc.contributor.author | Ming-Huan Lin | en |
| dc.contributor.author | 林明煥 | zh_TW |
| dc.date.accessioned | 2021-06-08T05:10:23Z | - |
| dc.date.copyright | 2011-10-03 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-20 | |
| dc.identifier.citation | 1. Andrew Wallace Hayes P.151, Principles and methods of toxicology, 5th edition, 2007
2. Athanasios Valavanidis, Thomails Vlachogianni & Constantinos Fiotakis (2009): 8-hydroxy-2′ -deoxyguanosine (8-OHdG): A Critical Biomarker of Oxidative Stress and Carcinogenesis, Journal of Environmental Science and Health, Part C, 27:2, 120-139 3. Carvalho VM et al. (1998) Formation of 1,N6-ethno-2,-deoxyadenosine adducts by trans, trans-2,4-decadienal. Chem. Res. Toxicol. 4. Chang et al. Toxicological science 2005; 87(2): 337-343) 5. Cherng SH, Huang KH, Yang SC, et al. Human 8-oxoguanine DNA glycosylase 1 mRNA expression as an oxidative stress exposure biomarker of cooking oil fumes. J Toxicol Environ Health A 2002;65: 265 – 78. 6. D J Howard, R B Ota, L A Briggs, et al. Environmental tobacco smoke in the workplace induces oxidative stress in employees, including increased production of 8-hydroxy-2'-deoxyguanosine. Cancer Epidemiol Biomarkers Prev 1998;7:141-146. 7. Louis W. Chang et al. Trans, Trans-2,4-Decadienal, a Product Found in Cooking Oil Fumes, Induces Cell Proliferation and Cytokine Production Due to Reactive Oxygen Species in Human Bronchial Epithelial Cells. Toxicological Sciences 87(2), 337-343 (2005). 8. Loureiro APM et al. (2000) trans, tran,-2,4-decadienal-induced 1,N2-ethno-2,-deoxyguanosine adduct formation. Chem. Res. 261:21-28. 9. Metayer et al. Cooking oil fumes and risk of lung cancer in women in rural Gansu, China. Lung Cancer, Volume 35, Issue 2, February 2002, Pages 111-117 10. Pin-pin Lin et al. Proteomic analysis of proteins associated with tt-DDE induced toxicity in BEA-2B cells. Biochemical and Biophysical Research Communications 376 (2008) 519-524. 11. Wah Kit LAM. Lung cancer in Asian women – the environment and genes. Respirology (2005) 10, 408-417. 12. Wu SC, Yen GC, Sheu F. Mutagenicity and identification of mutagenic compounds of fumes obtained from heating peanut oil. J Food Prot 2001;64:240-5. 13. Yaopu Pu, Lihong Yin, Yunhui Li, Pinpin Lin. Genotoxicity induced by cooking oil fume in rat pulmanory cells. Toxicology Letters, Volume 95, Supplement 1, July 1998, Page 42. 14. Y.C Chang et al. Toxicology and Applied Pharmacology 2007; 228: 76-83 15. Yun-Ching Chang et al. Trans, trans-2,4-decadienal induced cell proliferation via p27 pathway in human bronchial epithelial cells. Toxicology and Applied Pharmacology 228 (2008) 76-83 16. Ying-Chin Ko et al. Risk factors for primary lung cancer among non-smoking women in Taiwan. Int J Epidemiol 1997;26:24-31.Risk Factors for Primary Lung Cancer among Non-Smoking Women in Taiwan. 17. Ying-Chin Ko et al. Chinese Food Cooking and Lung Cancer in Women Nonsmokers. Am J Epidemiol 2000;151:140-7 18. Yu ITS, Chiu YI, Au JSK, Wong TW, Tang JL. (2006) Dose-response relationship between cooking fumes exposures and lung cancer among Chinese nonsmoking women. Cancer Res. 66(9) 4961-4967. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/23808 | - |
| dc.description.abstract | Lung cancer has been the first cause of death for a long time in Taiwan. Smoking is considered as the primary carcinogen, but the smoking rate of female is even lower in Taiwan. Epidemiology research shows that cooking oil fume is the significant risk factor of lung cancer.
Restaurant workers are study subjects of this research. There were 46 waiters, 59 cooks and 20 vendors recruited to join this study. Urine samples of pre-work and post-work were collected. We use tt-DDE (trans, trans-2,4-decadienal) to represent the exposure of cooking oil fume. Cooks and Vendors used personal air sampling equipment to collect the tt-DDE while they were working. We wanted to develop an LC-MS/MS analysis method for simultaneously quantification of urinary 8-OHdG and cotinine. To monitor the oxidative stress after the exposure of cooking oil fume. The simultaneously analysis method for quantification of urinary 8-OHdG and Cotinine has been established. Quantification range of cotinine is from 0.5ng/mL to 1000 ng/mL. The limit of detection (LOD) is 0.5ng/mL. Accuracy is from -0.22% to 3.76%. Precision is from 0.86% to 7.67%. The quantification range of 8-OHdG is from 0.5ng/mL to 15ng/mL. the limit of detection (LOD) is 0.5ng/mL. Accuracy is from -2.28% to 0.19%. Precision is from 1.42% to 8.24%. R2 values are larger than 0.995. Urinary 8-OHdG concentrations of cooks and vendors are higher than waiters (p-value <0.0001) and urinary 8-OHdG concentrations of post-work are higher than of pre-work. Urinary cotinine concentrations of cooks and vendors are also higher than waiters.( p-value<0.0001) | en |
| dc.description.provenance | Made available in DSpace on 2021-06-08T05:10:23Z (GMT). No. of bitstreams: 1 ntu-100-R97841021-1.pdf: 642370 bytes, checksum: a44f34c21af41970fc0822028f4faaa9 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 中文摘要 vi
Abstract： vii 第一章、目的 1 第二章、背景說明 2 2.1肺癌與環境因子 2 2.2 tt-DDE (trans,trans-2,4-Decadienal),反式-2,4-癸二烯醛 3 2.3 8-OHdG 4 2.4 Cotinine 6 2.5 樣品前處理方法 7 2.6分析及儀器原理 9 第三章、材料與方法開發 11 3.1 研究對象 11 3.2藥品與試劑 11 3.3儀器設備 13 3.4移動相配製 13 3.5線上固相萃取(On-Line Solid Phase Extraction)系統 14 3.6方法開發 16 3.7 統計分析 17 第四章 結果與討論 18 4.1 線上固相萃取系統方法開發 18 4.2 方法驗證 20 4.3 統計結果分析 22 4.4 結論與建議 23 參考文獻 52 附錄一、參與研究同意書 54 附錄二、問卷 55 | |
| dc.language.iso | zh-TW | |
| dc.title | 建立同步監測尿液中Cotinine與8-OHdG線上固相萃 取質譜分析方法探討餐飲業人員油煙暴露與氧化傷害指標之相關性。 | zh_TW |
| dc.title | The study of relationship between cooking oil fume exposure and DNA oxidative stress by simultaneous analysis of urinary Cotinine and 8-Hydroxy-2’-deoxyguanosine with On-line Solid Phase Extraction Isotope-dilution and LC-MS/MS. | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 鄭尊仁,陳家揚 | |
| dc.subject.keyword | tt-DDE,8-OHdG,Cotinine,線上固相萃取,質譜儀,油煙, | zh_TW |
| dc.subject.keyword | tt-DDE,8-OHdG,Cotinine,On-line SPE LC/MS/MS,cooking oil fume, | en |
| dc.relation.page | 58 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2011-08-20 | |
| dc.contributor.author-college | 公共衛生學院 | zh_TW |
| dc.contributor.author-dept | 職業醫學與工業衛生研究所 | zh_TW |
| 顯示於系所單位： | 職業醫學與工業衛生研究所 | |
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