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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22508
Title: | 口腔鏈球菌與人類口腔癌細胞,白血球,及癌症相關纖維母細胞之交互作用 Interactions of Oral Streptococci with Human Oral Cancer Cells, Leukocytes and Cancer-Associated Fibroblasts |
Authors: | Min-Jie Tsai 蔡旻潔 |
Advisor: | 賈景山(Jean-San Chia) |
Keyword: | 口腔癌,口腔共生鏈球菌,單核球,癌症相關纖維母細胞,發炎反應, oral cancer,oral commensal streptococci,monocyte,cancer-associated fibroblast,inflammation, |
Publication Year : | 2010 |
Degree: | 碩士 |
Abstract: | In the particular microenvironment of oral squamous cell carcinoma (OSCC), the orchestra of cancer cells, infiltrating immune cells, certain bacteria (such as Streptococcus anginosus), and stromal fibroblasts (cancer-associated fibroblasts, CAFs) play a symphony of cancer-related inflammation. In oral cancer, the role of CAFs in modulating the cancer-assoicated inflammation remains poorly understood. The CAFs isolated and cultured from oral cancer tissues are characterized with human fibroblast markers. Recombinant human IL-1β activates CAFs in a dose-dependent manner, and the production of IL-6, IL-8 and MCP-1 in supernatant accumulates as the stimulation time increases. Recombinant IL-1β-activated CAFs express increased levels of proinflammtory cytokines (such as IL-6), and chemokines which are associated with cancer progression, and recruitment of monocytes/macrophages (including IL-8, MCP-1, MCP-3, and GM-CSF). Stimulation of OSCC cells and peripheral blood mononuclear cells with S. anginosus induces the factors which activate CAFs in an IL-1-dependent manner. Conditioned media of CAF promote migration of human monocytes, and IL-1β activation enhances the chemotactic effect. Taken together, both the interaction with invaded streptococci as the exogenous stimuli for immune cells and the endogenous property of OSCC cells lead to IL-1 production. Activation of stromal fibroblasts by IL-1 may give rise to tumor-promoting inflammation in oral cancer microenvironment. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/22508 |
Fulltext Rights: | 未授權 |
Appears in Collections: | 微生物學科所 |
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ntu-99-1.pdf Restricted Access | 4.82 MB | Adobe PDF |
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