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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/21836
標題: | 自組裝C60富勒烯奈米薄片上培養細菌視紫質轉染的人類纖維母細胞之光遺傳學調控和重新編程 Optogenetic modulation and reprogramming of bacteriorhodopsin-transfected human fibroblasts on self-assembled fullerene C60 nanosheets |
作者: | PEI-WEN LUO 羅佩玟 |
指導教授: | 徐善慧(Shan-hui Hsu) |
關鍵字: | 富勒烯C60,自組裝奈米薄片,光遺傳學,細菌視紫質,神經重新編程, fullerene C60,self-assembled nanosheet,optogenetics,bacteriorhodopsin,neural reprogramming, |
出版年 : | 2018 |
學位: | 碩士 |
摘要: | 富勒烯因為具有獨特的生物相容性和光電性質,因此在生物醫學領域中被廣泛的應用。另一方面,自然界中也有許多細胞膜蛋白,如細菌視紫質也同樣具有特殊的光電性質,高度表現的細菌視紫質(Highly expressible bacteriorhodopsin, HEBR)是一種新型的光敏蛋白,具有通過光遺傳學調節引發神經活動的潛力。在本研究中,我們將HEBR質體遞送至人類纖維母細胞中,並將其細胞培養在二維C60富勒烯自組裝奈米薄片上誘導其朝向神經分化。結果表明,上述方法結合綠光刺激(每天3次,一次持續3秒),可以在沒有任何神經誘導培養基的情況下促進人類纖維母細胞在7天內重新編程並分化成類神經細胞。將C60富勒烯作為培養基材,結合透過綠光刺激的HEBR進入細胞內,可提供新的光遺傳學平台,透過調節空間(C60奈米薄片)與時間(光照率)使人類纖維母細胞朝向類神經細胞發展,期待未來可應用於神經再生的組織工程。 Fullerenes have the unique biocompatibility and photoelectric properties and are candidate materials for biomedical applications. Several cell membrane proteins in nature such as bacteriorhodopsin also have photoelectric properties. Highly expressible bacteriorhodopsin (HEBR) is a novel light-sensitive opsin that has the potential to trigger neural activities through optogenetic modulation. In this study, we delivered HEBR plasmids to human fibroblasts and exposed the cells to C60 fullerene self-assembled two-dimensional nanosheets. Results showed that the above approach combined with light stimulation (3 second duration and three times per day) may promote the reprogramming and differentiation of human fibroblasts into neural-like cells in 7 days without any neural induction medium. The special photoelectric properties of fullerenes as culture substrates and transfected HEBR on cell membrane may provide a new optogenetic platform for regulating the location (C60 nanosheet) and time (frequency of light illumination) for human fibroblasts to become neural-like cells, and may be applied to improve neural regeneration in the future. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/21836 |
DOI: | 10.6342/NTU201804346 |
全文授權: | 未授權 |
顯示於系所單位: | 高分子科學與工程學研究所 |
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