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標題: | 癌細胞攝取金奈米環與侷域表面電漿子共振導致滅活效率和金奈米環大小及濃度的關係 Dependencies of Cancer Cell Uptake and Localized Surface Plasmon Resonance Induced Inactivation Efficiencies on Au Nanoring Size and Concentration |
作者: | Yu-Wei Chang 張祐維 |
指導教授: | 楊志忠 |
關鍵字: | 攝取,侷域表面電漿子,奈米顆粒,效率,細胞, Cell Uptake,Localized Surface Plasmon Resonance,Inactivation,Efficiencies,nanoparticles, |
出版年 : | 2014 |
學位: | 碩士 |
摘要: | 首先,我們研究金奈米環的大小、與細胞作用時間和濃度對於SCC4癌細胞吞噬效率的影響。然後,藉由波長接近金奈米環共振波長的激發,我們使用不同大小和濃度之金奈米環讓SAS癌細胞攝取來進行侷域表面電漿子共振所導致的光熱滅活實驗。在SCC4癌細胞吞噬金奈米環效率的研究中,當具有抗體金奈米環的外直徑小於100奈米時,SCC4癌細胞吞噬效率明顯增加。藉由改變不同的作用時間,在8小時條件下,我們發現SCC4癌細胞吞噬奈米粒子數達到最大值,之後就開始胞吐作用。當金奈米環的濃度達到臨界值時,我們發現吞噬效率也顯著增加。在使用生物耦聯之金奈米環來對SAS癌細胞滅活之研究中,我們發現即使細胞攝取小尺寸的金奈米環數比大尺寸的金奈米環數小、且共振波長略遠於雷射波長,小尺寸的金奈米環仍具有較強的光熱效果或是較高的癌細胞滅活效率。小尺寸的金奈米環具有較強的光熱作用部份源於相對於散射截面積,其具有較大的吸收截面積。對於SCC4或SAS口腔癌細胞,當使用外徑小於100奈米的金奈米環,吞噬和滅活效率都較高。 We first study the dependencies of SCC4 cancer cell uptake efficiency on Au nanoring (NRI) size, incubation time, and Au NRI concentration. Then, we perform the experiment of localized surface plasmon (LSP)-induced photothermal inactivation of SAS cancer cells with different Au NRI sizes and concentrations through the illumination of a laser of a wavelength close to the LSP resonance peaks of the NRIs. In the study of NRI uptake efficiency, which includes the adsorption and internalization efficiencies of NRI by SCC4 cells, it is found that the uptake efficiency becomes significantly higher when the outer diameter is smaller than ~100 nm and antibody is linked to NRI. By varying the incubation time, it is found that the uptake of SCC4 cells reaches a maximum level at 8 hours and then starts effective exocytosis. Regarding the uptake dependence on NRI concentration, it is found that the uptake efficiency increases significantly when NRI concentration reaches a threshold level. In the inactivation study of SAS cancer cells with bio-conjugated Au NRI, it is found that the small-sized NRI has higher uptake efficiency and always has a stronger photothermal effect or higher cancer cell inactivation efficiency, even though the concentration of the small-sized NRI is slightly smaller and its LSP resonance peak is slightly further away from the laser wavelength, when compared with the large-sized NRI. The stronger photothermal effect of a small-sized NRI is partly attributed to its relatively larger absorption cross section with respect to scattering cross section. For either SCC4 or SAS oral cancer cells, the uptake and inactivation efficiencies are higher when Au NRIs of <100 nm in outer diameter are used. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/18793 |
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顯示於系所單位: | 光電工程學研究所 |
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