請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/18769完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 謝文陽 | |
| dc.contributor.author | Chin-Yu Chen | en |
| dc.contributor.author | 陳慶瑜 | zh_TW |
| dc.date.accessioned | 2021-06-08T01:24:50Z | - |
| dc.date.copyright | 2014-08-04 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-01 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/18769 | - |
| dc.description.abstract | 本篇研究從宜蘭大溪漁港中的海水，分離出三株具脂肪分解活性的 Alteromonas species D3、D5 和 D8，其中 Alteromonas D5 所產的脂肪分解酶具有熱穩定性及鹼穩定性，並經由硫酸銨沉澱及陰離子交換層析將酵素部分純化，最終酵素產量為0.11 %，純化倍率為 15.6。螢光活性呈色顯現出 5 個條帶具有活性，SDS-PAGE 顯示出含量最多的酵素分子量約為 30 kDa，但最高活性的酵素分子量約為4 0 kDa。酵素活性最好的條件為 70℃ 及 pH 8，在 20-80 ℃ 及 pH 6-9 都具有活性；多種金屬離子、抑制劑及界面活性劑的測試用來評估酵素的特性；經由實驗觀察，酵素能催化約 90 % 的棕櫚酸與甲醇反應並轉換成酯類，且酵素與四種市售清潔劑混合處理後能維持 80 % 以上的活性。根據本篇研究，此脂肪分解酶具有工業應用的潛力。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-08T01:24:50Z (GMT). No. of bitstreams: 1 ntu-103-R01241209-1.pdf: 1063412 bytes, checksum: ad946ef0bf9ea97a77c37352d68de858 (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | 中文摘要 v
Abstract vi 第一章 前言 1 第一節、細菌所產脂肪分解酶之分類 1 第二節、脂肪分解酶的應用 2 第三節、從微生物取得脂肪分解酶之優點 4 第四節、研究動機 6 第二章 材料和方法 7 第一節、實驗材料與設備 7 第二節、實驗所使用之培養基 9 第三節、細菌樣本 10 第四節、分離、培養與篩選菌株 10 第五節、菌株之分泌脂肪分解酶檢測 10 第六節、蛋白質定量 11 第七節、脂肪分解酶活性測試 11 第八節、分離株形態與生理生化測試 12 第九節、分離株之 16S rRNA 序列分析 17 第十節、脂肪分解酶的純化 19 第十一節、脂肪分解酶的生化特性分析 22 第十二節、脂肪分解酶的應用 25 第十三節、統計分析 27 第三章 結果 28 第一節、菌株分離與細菌脂肪分解酶的活性測試 28 第二節、分離株之脂肪分解酶活性大小 28 第三節、分離株形態與生理生化特性 29 第四節、分離株之 16S rRNA基因序列分析 30 第五節、分離株之 D5 培養條件測試 30 第六節、脂肪分解酶的純化 31 第七節、脂肪分解酶的生化特性分析 32 第八節、脂肪分解酶的應用 34 第四章 討論 36 第一節、海洋細菌Alteromonas species 分離株的鑑定 36 第二節、脂肪分解酶的純化和特性探討 37 第三節、脂肪分解酶的應用 40 第五章 結論 42 參考資料 43 | |
| dc.language.iso | zh-TW | |
| dc.title | 海洋菌株 Alteromonas sp. D5 所產脂肪分解酶之部分純化及探討 | zh_TW |
| dc.title | Partial purification and characterization of lipolytic enzymes from a marine bacterium Alteromonas sp. D5 | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳卓昇,簡文達,李宗徽,陳逸民 | |
| dc.subject.keyword | Alteromonas,海洋細菌,脂肪分解?,熱穩定性,鹼穩定性,陰離子交換層析,螢光活性呈色,SDS-PAGE,酯化能力,耐受清潔劑, | zh_TW |
| dc.subject.keyword | Alteromonas,marine bacteria,lipolytic enzyme,thermostable,alkaline stable,anion exchange chromatography,fluorescent zymogram,SDS-PAGE,esterification,detergent compatible, | en |
| dc.relation.page | 76 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2014-08-01 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 海洋研究所 | zh_TW |
| 顯示於系所單位： | 海洋研究所 | |
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