請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/18437完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 朱家瑩(Chia-Ying Chu) | |
| dc.contributor.author | Hung-Yi Yeh | en |
| dc.contributor.author | 葉弘毅 | zh_TW |
| dc.date.accessioned | 2021-06-08T01:05:10Z | - |
| dc.date.copyright | 2014-09-03 | |
| dc.date.issued | 2014 | |
| dc.date.submitted | 2014-08-20 | |
| dc.identifier.citation | Baguna, J., & Slack, J. M. W. (1981). Planarian Neoblasts. Nature, 290(5801), 14-15. doi: Doi 10.1038/290014b0
Beck, C. W., & Slack, J. M. (1998). Analysis of the developing Xenopus tail bud reveals separate phases of gene expression during determination and outgrowth. Mech Dev, 72(1-2), 41-52. Beck, C. W., & Slack, J. M. (1999). A developmental pathway controlling outgrowth of the Xenopus tail bud. Development, 126(8), 1611-1620. Beck, C. W., Whitman, M., & Slack, J. M. (2001). The role of BMP signaling in outgrowth and patterning of the Xenopus tail bud. Dev Biol, 238(2), 303-314. doi: 10.1006/dbio.2001.0407 Bode, H. R. (2003). Head regeneration in Hydra. Dev Dyn, 226(2), 225-236. doi: 10.1002/dvdy.10225 Castrillon, D. H., Quade, B. J., Wang, T. Y., Quigley, C., & Crum, C. P. (2000). The human VASA gene is specifically expressed in the germ cell lineage. Proc Natl Acad Sci U S A, 97(17), 9585-9590. doi: 10.1073/pnas.160274797 Chera, S., Ghila, L., Wenger, Y., & Galliot, B. (2011). Injury-induced activation of the MAPK/CREB pathway triggers apoptosis-induced compensatory proliferation in hydra head regeneration. Dev Growth Differ, 53(2), 186-201. doi: 10.1111/j.1440-169X.2011.01250.x Chuma, S., & Nakano, T. (2013). piRNA and spermatogenesis in mice. Philos Trans R Soc Lond B Biol Sci, 368(1609), 20110338. doi: 10.1098/rstb.2011.0338 Dial, B. E., & Fitzpatrick, L. C. (1983). Lizard Tail Autotomy: Function and Energetics of Postautotomy Tail Movement in Scincella lateralis. Science, 219(4583), 391-393. doi: 10.1126/science.219.4583.391 Eisenhoffer, G. T., Kang, H., & Sanchez Alvarado, A. (2008). Molecular analysis of stem cells and their descendants during cell turnover and regeneration in the planarian Schmidtea mediterranea. Cell Stem Cell, 3(3), 327-339. doi: 10.1016/j.stem.2008.07.002 Fleming, P. A., Verburgt, L., Scantlebury, M., Medger, K., & Bateman, P. W. (2009). Jettisoning ballast or fuel? Caudal autotomy and locomotory energetics of the Cape dwarf gecko Lygodactylus capensis (Gekkonidae). Physiol Biochem Zool, 82(6), 756-765. doi: 10.1086/605953 Gentile, L., Cebria, F., & Bartscherer, K. (2011). The planarian flatworm: an in vivo model for stem cell biology and nervous system regeneration. Dis Model Mech, 4(1), 12-19. doi: 10.1242/dmm.006692 Gonzalez-Estevez, C., Felix, D. A., Rodriguez-Esteban, G., & Aboobaker, A. A. (2012). Decreased neoblast progeny and increased cell death during starvation-induced planarian degrowth. Int J Dev Biol, 56(1-3), 83-91. doi: 10.1387/ijdb.113452cg Guedelhoefer, O. C. th, & Sanchez Alvarado, A. (2012). Planarian immobilization, partial irradiation, and tissue transplantation. J Vis Exp(66). doi: 10.3791/4015 Gustafson, E. A., & Wessel, G. M. (2010). Vasa genes: emerging roles in the germ line and in multipotent cells. Bioessays, 32(7), 626-637. doi: 10.1002/bies.201000001 Hauenschild, C. (1965). [the Endocrine Control of Reproduction in Annelids]. Arch Anat Microsc Morphol Exp, 54, 429-452. Hay, E. D., & Coward, S. J. (1975). Fine structure studies on the planarian, Dugesia. I. Nature of the 'neoblast' and other cell types in noninjured worms. J Ultrastruct Res, 50(1), 1-21. Hayashi, T., & Agata, K. (2012). A unique FACS method to isolate stem cells in planarian. Methods Mol Biol, 879, 29-37. doi: 10.1007/978-1-61779-815-3_2 Hayashi, T., Shibata, N., Okumura, R., Kudome, T., Nishimura, O., Tarui, H., & Agata, K. (2010). Single-cell gene profiling of planarian stem cells using fluorescent activated cell sorting and its 'index sorting' function for stem cell research. Dev Growth Differ, 52(1), 131-144. doi: 10.1111/j.1440-169X.2009.01157.x Jiang, H., Patel, P. H., Kohlmaier, A., Grenley, M. O., McEwen, D. G., & Edgar, B. A. (2009). Cytokine/Jak/Stat signaling mediates regeneration and homeostasis in the Drosophila midgut. Cell, 137(7), 1343-1355. doi: 10.1016/j.cell.2009.05.014 King, R. S., & Newmark, P. A. (2012). The cell biology of regeneration. J Cell Biol, 196(5), 553-562. doi: 10.1083/jcb.201105099 Kobayashi, C., Saito, Y., Ogawa, K., & Agata, K. (2007). Wnt signaling is required for antero-posterior patterning of the planarian brain. Dev Biol, 306(2), 714-724. doi: 10.1016/j.ydbio.2007.04.010 Kobayashi, C., Watanabe, K., & Agata, K. (1999). The process of pharynx regeneration in planarians. Dev Biol, 211(1), 27-38. doi: 10.1006/dbio.1999.9291 Kobayashi, K., & Hoshi, M. (2002). Switching from asexual to sexual reproduction in the planarian Dugesia ryukyuensis: change of the fissiparous capacity along with the sexualizing process. Zoolog Sci, 19(6), 661-666. Krichinskaya, E. B., & Martynova, M. G. (1975). Distribution of neoblasts and mitoses during the asexual reproduction of the planarian Dugesia tigrina (Girard). Sov J Dev Biol, 5(4), 309-314. Lasko, P. (2013). The DEAD-box helicase Vasa: evidence for a multiplicity of functions in RNA processes and developmental biology. Biochim Biophys Acta, 1829(8), 810-816. doi: 10.1016/j.bbagrm.2013.04.005 Leroy, P., Seboun, E., Mattei, M. G., Fellous, M., & Bishop, C. E. (1987). Testis-specific transcripts detected by a human Y-DNA-derived probe. Development, 101 Suppl, 177-183. Meikar, O., Vagin, V. V., Chalmel, F., Sostar, K., Lardenois, A., Hammell, M., . . . Kotaja, N. (2014). An atlas of chromatoid body components. RNA, 20(4), 483-495. doi: 10.1261/rna.043729.113 Mochii, M., Taniguchi, Y., & Shikata, I. (2007). Tail regeneration in the Xenopus tadpole. Dev Growth Differ, 49(2), 155-161. doi: 10.1111/j.1440-169X.2007.00912.x Morgen, Thomas Hunt. (1901). regeneration. Newmark, P. A., & Sanchez Alvarado, A. (2002). Not your father's planarian: a classic model enters the era of functional genomics. Nat Rev Genet, 3(3), 210-219. doi: 10.1038/nrg759 Ogawa, K., Wakayama, A., Kunisada, T., Orii, H., Watanabe, K., & Agata, K. (1998). Identification of a receptor tyrosine kinase involved in germ cell differentiation in planarians. Biochem Biophys Res Commun, 248(1), 204-209. doi: 10.1006/bbrc.1998.8915 Orii, H., Sakurai, T., & Watanabe, K. (2005). Distribution of the stem cells (neoblasts) in the planarian Dugesia japonica. Dev Genes Evol, 215(3), 143-157. doi: 10.1007/s00427-004-0460-y Pearson, B. J., Eisenhoffer, G. T., Gurley, K. A., Rink, J. C., Miller, D. E., & Sanchez Alvarado, A. (2009). Formaldehyde-based whole-mount in situ hybridization method for planarians. Dev Dyn, 238(2), 443-450. doi: 10.1002/dvdy.21849 Pearson, B. J., & Sanchez Alvarado, A. (2010). A planarian p53 homolog regulates proliferation and self-renewal in adult stem cell lineages. Development, 137(2), 213-221. doi: 10.1242/dev.044297 Petersen, C. P., & Reddien, P. W. (2008). Smed-betacatenin-1 is required for anteroposterior blastema polarity in planarian regeneration. Science, 319(5861), 327-330. doi: 10.1126/science.1149943 Petersen, C. P., & Reddien, P. W. (2009). A wound-induced Wnt expression program controls planarian regeneration polarity. Proc Natl Acad Sci U S A, 106(40), 17061-17066. doi: 10.1073/pnas.0906823106 Reddien, P. W., & Sanchez Alvarado, A. (2004). Fundamentals of planarian regeneration. Annu Rev Cell Dev Biol, 20, 725-757. doi: 10.1146/annurev.cellbio.20.010403.095114 Resch, A. M., Palakodeti, D., Lu, Y. C., Horowitz, M., & Graveley, B. R. (2012). Transcriptome analysis reveals strain-specific and conserved stemness genes in Schmidtea mediterranea. PLoS One, 7(4), e34447. doi: 10.1371/journal.pone.0034447 Rosner, A., & Rinkevich, B. (2007). The DDX3 subfamily of the DEAD box helicases: divergent roles as unveiled by studying different organisms and in vitro assays. Curr Med Chem, 14(23), 2517-2525. Rouhana, L., Shibata, N., Nishimura, O., & Agata, K. (2010). Different requirements for conserved post-transcriptional regulators in planarian regeneration and stem cell maintenance. Dev Biol, 341(2), 429-443. doi: 10.1016/j.ydbio.2010.02.037 Rouhana, L., Weiss, J. A., Forsthoefel, D. J., Lee, H., King, R. S., Inoue, T., . . . Newmark, P. A. (2013). RNA interference by feeding in vitro-synthesized double-stranded RNA to planarians: methodology and dynamics. Dev Dyn, 242(6), 718-730. doi: 10.1002/dvdy.23950 Shibata, N., Umesono, Y., Orii, H., Sakurai, T., Watanabe, K., & Agata, K. (1999). Expression of vasa(vas)-related genes in germline cells and totipotent somatic stem cells of planarians. Dev Biol, 206(1), 73-87. doi: 10.1006/dbio.1998.9130 Slack, J. M., Beck, C. W., Gargioli, C., & Christen, B. (2004). Cellular and molecular mechanisms of regeneration in Xenopus. Philos Trans R Soc Lond B Biol Sci, 359(1445), 745-751. doi: 10.1098/rstb.2004.1463 Solana, J., Kao, D., Mihaylova, Y., Jaber-Hijazi, F., Malla, S., Wilson, R., & Aboobaker, A. (2012). Defining the molecular profile of planarian pluripotent stem cells using a combinatorial RNAseq, RNA interference and irradiation approach. Genome Biol, 13(3), R19. doi: 10.1186/gb-2012-13-3-r19 Solana, J., & Romero, R. (2009). SpolvlgA is a DDX3/PL10-related DEAD-box RNA helicase expressed in blastomeres and embryonic cells in planarian embryonic development. Int J Biol Sci, 5(1), 64-73. Tanaka, S. S., Toyooka, Y., Akasu, R., Katoh-Fukui, Y., Nakahara, Y., Suzuki, R., . . . Noce, T. (2000). The mouse homolog of Drosophila Vasa is required for the development of male germ cells. Genes Dev, 14(7), 841-853. Vong, Q. P., Li, Y., Lau, Y. F., Dym, M., Rennert, O. M., & Chan, W. Y. (2006). Structural characterization and expression studies of Dby and its homologs in the mouse. J Androl, 27(5), 653-661. doi: 10.2164/jandrol.106.000471 Wagner, D. E., Ho, J. J., & Reddien, P. W. (2012). Genetic regulators of a pluripotent adult stem cell system in planarians identified by RNAi and clonal analysis. Cell Stem Cell, 10(3), 299-311. doi: 10.1016/j.stem.2012.01.016 Wang, B., Collins, J. J., 3rd, & Newmark, P. A. (2013). Functional genomic characterization of neoblast-like stem cells in larval Schistosoma mansoni. Elife, 2, e00768. doi: 10.7554/eLife.00768 Wenemoser, D., & Reddien, P. W. (2010). Planarian regeneration involves distinct stem cell responses to wounds and tissue absence. Dev Biol, 344(2), 979-991. doi: 10.1016/j.ydbio.2010.06.017 Williams, D. D. (1959). Limb regeneration in the salamander, Triturus viridescens, after large initial and during prolonged smaller injections of cortisone acetate. Endocrinology, 64(2), 292-296. doi: 10.1210/endo-64-2-292 Zeeman, A. M., Stoop, H., Boter, M., Gillis, A. J., Castrillon, D. H., Oosterhuis, J. W., & Looijenga, L. H. (2002). VASA is a specific marker for both normal and malignant human germ cells. Lab Invest, 82(2), 159-166. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/18437 | - |
| dc.description.abstract | 再生是生物進行修復或重新長出失去的組織時重要的步驟。渦蟲因富含多能性幹細胞賦予其優秀的再生能力故常作為再生研究的物種。在渦蟲的再生過程中，成體幹細胞會移動到傷口處，並進行分裂分化補充失去的組織。截至今日，雖然有許多研究在探討再生的問題，但再生的完整分子機制仍未清楚。Vasa是一種DEAD-box RNA 解旋酶，在果蠅以及老鼠已證實vasa參與調控生殖幹細胞精化的過程。另一群與之相似的DDX3蛋白稱為Vasa-like gene (vlg)，過去已發現vasa-like gene會表現於渦蟲幹細胞中;而在Schmidtea polychroa則是表現於有高度分裂潛力的胚胎細胞，在成熟的個體中此細胞會分裂分化成精細胞。本研究目的在探討渦蟲體內的vlg蛋白是否能藉由調控幹細胞的生長與分化而參與再生過程。本研究中我發現了兩個新穎的、有別於已發表的渦蟲vlg基因(DjvlgA-v1及-v2)，且此兩種DjvlgA基因皆表現於渦蟲幹細胞neoblasts中。以RNAi方式降低vasa-like gene表現之後，造成渦蟲再生遲緩，且降低渦蟲體內分化前驅細胞的比例以及前驅細胞標誌基因p53的表現量。綜合以上結果，DjvlgA蛋白在可能參與渦蟲再生過程中調控幹細胞分化成早期前驅細胞的步驟。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-08T01:05:10Z (GMT). No. of bitstreams: 1 ntu-103-R01b41002-1.pdf: 5999093 bytes, checksum: 51a46d4a2f38f332aa5360619ea7acfd (MD5) Previous issue date: 2014 | en |
| dc.description.tableofcontents | Contents
1. Introduction 1 1.1 Regeneration 1 1.2 Processes and Mechanisms of regeneration 1 1.3 Planarian and regeneration 2 1.4 Neoblasts and chromatoid body 4 1.5 Vasa and Vasa-like gene 7 2. Material methods 11 2.1 Animals 11 2.2 PCR 11 2.3 Quantitative-PCR 12 2.4 cDNA cloning for Dugesia japonica vasa-like gene A 12 2.5 Irradiation 13 2.6 Whole-mount in situ hybridization 13 2.7 RNA interference 15 2.8 Fluorescence-activated cell sorting 15 2.9 Immunostaining with H3P 16 3. Results 17 3.1 Isolation of Dugesia japonica vasa-like gene A 17 3.2 Expression of DjvlgA-v1and DjvlgA-v2 in sexual and asexual strains 18 3.3 Expression pattern of DjvlgA-v1 and DjvlgA-v2 19 3.4 DjvlgA-v2 is expressed more in neoblasts 20 3.5 DjvlgA-v2 may participate in regeneration 22 3.6 Knockdown of DjvlgA-v2 results in delayed regeneration 22 3.7 DjvlgA-v2-RNAi planarians has no significant change in cell proliferation 23 3.8 Dissecting the mechanism of regeneration delay in DjvlgA-v2-depleted planarians 24 3.9 DjvlgA-vs-RNAi have no effect on planarian homeostasis 25 4. Discussion 27 4.1 DjvlgA-v1 and DjvlgA-v2 are classified into PL10 protein family 27 4.2 DjvlgA-v genes are strain specific genes 28 4.3 DjvlgA-v1 and DjvlgA-v2 may be expressed in neoblasts and some irradiation-insensitive cells 29 4.4 DjvlgA-v2 may participate in regeneration 31 4.5 The change proliferating cells in DjvlgA-v2-RNAi planarians 32 4.6 The change of cell-lineage marker in DjvlgA-v2-RNAi planarians 32 4.7 The hypothesis of DjvlgA-v1 and DjvlgA-v2 function in planarians 33 5. Figures 36 6. Supplementary Figures 57 7. References 63 | |
| dc.language.iso | en | |
| dc.title | 探討Vasa-like gene 在渦蟲再生中扮演的角色 | zh_TW |
| dc.title | Study the role of Vasa-like gene in planarian regeneration | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 102-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳俊宏(Jiun-Hong Chen),郭典翰(Dian-Han Kuo) | |
| dc.subject.keyword | 渦蟲,再生,vasa-like gene,DDX3,幹細胞,早期前驅細胞, | zh_TW |
| dc.subject.keyword | Planarians,regeneration,vasa-like gene,DDX3,neoblasts,early progeny cells, | en |
| dc.relation.page | 71 | |
| dc.rights.note | 未授權 | |
| dc.date.accepted | 2014-08-20 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生命科學系 | zh_TW |
| 顯示於系所單位： | 生命科學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-103-1.pdf 目前未授權公開取用 | 5.86 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。