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Title: | 原位雜合反應顯示豬鐵士古病毒可感染前所未知的豬組織細胞型別 In Situ Hybridization Demonstrates That Porcine Teschovirus Can Infect a Wider Variety of Tissue Cell Types That Has Not Been Revealed |
Authors: | Ya-Mei Chen 陳雅媚 |
Advisor: | 王汎熒 |
Keyword: | 豬鐵士古病毒,原位雜合反應, porcine teschovirus,ISH,in situ hybridization, |
Publication Year : | 2014 |
Degree: | 碩士 |
Abstract: | 豬鐵士古病毒(porcine teschoviruses, PTVs)為單股正向RNA病毒,分類為teschovirus屬picornaviridae科。PTV感染會造成多種豬隻疾病;包括非化膿性灰質腦脊髓炎、肺炎、繁殖障礙、腸炎等。PTV在台灣首次於2000年分離出來,目前台灣豬場呈現PTV高度汙染且廣泛分布。本研究目的為探討在地方性感染的豬隻中PTV所可能扮演的致病角色。本實驗室先前收集29頭淘汰豬隻不同臟器並藉巢式聚合酶鏈鎖反應(nested RT-PCR)檢測病毒分布。96.7% 豬隻呈現 PTV陽性,臟器中病毒量最高的為腸道、其次為淋巴器官、腦及內臟。統計顯示,在腦的後段之非化膿性腦炎的出現與 PTV感染具有相關性 (P = 0.054)。本研究則利用原位雜合反應(in situ hybridization, ISH)染色偵測PTV在組織器官中分布的位置。結果顯示PTV廣泛分布於神經系統、淋巴系統、腎臟、空腸、迴腸及結腸內,ISH可偵測到PTV廣泛分佈之前未知的細胞型別,使我們對PTV的致病性有進一步的了解。本研究建立偵測豬鐵士古病毒RNA之原位雜合染色技術並藉以確認病毒的細胞親和性。 Porcine teschoviruses (PTVs) are RNA viruses that are classified within the genus Teschovirus of the family Picornaviridae and cause non-suppurative meningoencephalitis and polioencephalomyelitis. In Taiwan, the PTV infection is the first or secondary swine viral diseases and usually isolated together with other common pathogens. To investigate PTV distribution, tissues were collected from 29 culled postweaning pigs, and the positive rate of PTV detection by nested RT-PCR was 96.7% (by heads). The most positive tissues were the intestines where ileum had the significantly highest detection rate, followed by lymphoid organs, cranial portion of the brain, and visceral organs. Statistics showed that nonsuppurative encephalitis in the caudal brain had an obvious correlation with PTV detection. To recognize the relation between lesions and PTV locations, in situ hybridization (ISH) was performed. PTV positive signals were presented in the nervous system, immune system, kidney, jejunum, ileum and colon. PTVs are able to infect a wider variety of cell types than what has been known. This study furthers our understanding on the pathogenicity and pathogenesis of PTV. The study establishes in situ hybridization technique for PTVs detection and confirms the PTV cell tropism. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/17806 |
Fulltext Rights: | 未授權 |
Appears in Collections: | 獸醫學系 |
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ntu-103-1.pdf Restricted Access | 3.07 MB | Adobe PDF |
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