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標題: | 利用Rhodococcus erythropolis NTU-1細胞破乳化 De-emulsification by Rhodococcus erythropolis NTU-1 |
作者: | Shih-Wen Wang 王詩雯 |
指導教授: | 劉懷勝 |
關鍵字: | 破乳化,Rhodococcus erythropolis NTU-1菌株,原油回收量,乳化液,Tween80, Emulsion,tertiary oil recovery,de-emulsification,Rhodococcus erythropolis NTU-1, |
出版年 : | 2014 |
學位: | 碩士 |
摘要: | 本研究目的主要探討R. erythropolis NTU-1之破乳化的特性,其能利用此菌株使油水乳化液在短時間內能分離出油。
以菌液破乳化的研究結果發現,乳化劑Tween80可以將正十六烷與水互相混合形成白色乳化液,利用NTU-1菌液可以在24小時內可以回收85%以上的正十六烷,破乳化效率也高達90%。同時發現在O/W/T為2~8/1/0.01,並不影響最適添加NTU-1菌液量(3 mL,OD600=1)。 若以烘乾經正十六烷培養的NTU-1結塊細胞破乳化,該細胞粉末雖然不具活性,但仍保有很高的疏水性,亦可以使破乳化效果在12小時內就達85%以上且回收85%以上的正十六烷。同時,在O/W/T為2~9/1/0.01,並不影響乾燥NTU-1結塊的最適添加量(0.03 g)。經實驗可估算出1 g的乾燥結塊約可以回收250 mL 的正十六烷。 實驗發現在低乳化劑濃度0.2%或油/水(O/W)比值越小或高破乳化溫度下,可以提高破乳化程度及縮短破乳化時間。另外,藉由改變不同乳化劑體積,發現最適添加NTU-1細胞(不論是菌液或是乾燥結塊)與Tween80體積存在面積比(量的n=2/3次方)的關係。 由此可知,NTU-1菌液或是烘乾後的NTU-1結塊皆具有很好的破乳化能力,可以在24小時就回收80%以上的正十六烷,此項成果確實在三級原油回收方面,提供一個相當具有競爭性及發展性的方法。 This report investigated the feasibility of applying Rhodococcus erythropolis NTU-1 that possessed de-emulsifying ability to recover oil from oil-in-water (O/W) emulsion. Suspended NTU-1 cell reach de-emulsification efficiency up to 90% and recovered 85% oil in 24 hr. Besides, the optimal amount of NTU-1 cell addition was 3 mL (OD600=1) when Oil/Water/Tween80 was 2~8/1/0.01. Heat-dried bioflocculi also were tested because of its convenience in practical application. Dried bioflocculi retained its high hydrophobicity, resulting in more than 85% de-emulsification and 85% oil recovery in 12hr. When Oil/Water/Tween80 volume is 2~9/1/0.01, the optimal amount of dried NTU-1 was 0.03 g. It was estimated and calculated that 250 mL oil might be recovered by 1 g dried bioflocculi. Lower Tween80 concentration or O/W ratio and higher temperature, better de-emulsification and oil recovery in less time. In addition, the optimal amount of NTU-1 for de-emulsification, for both suspended and dried NTU-1, was proportional to the amount of Tween80 to the power of 2/3, indicating a surface area dependent behavior. These experimental results showed that NTU-1 in MSM or dried bioflocculi had a potential role in enhancing tertiary oil recovery process. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16660 |
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顯示於系所單位: | 化學工程學系 |
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