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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16188
標題: | 阿拉伯芥AtbHLH147基因功能性分析 Functional analysis of AtbHLH147 in Arabidopsis |
作者: | Ko-Ching Wang 王科晶 |
指導教授: | 吳克強 |
關鍵字: | 阿拉伯芥,bHLH蛋白,生長素,吉貝素,葉綠素,光型態發生, Arabidopsis,bHLH147,Auxin,Gibberellin,Chlorophyll,Photomorphogenesis, |
出版年 : | 2012 |
學位: | 碩士 |
摘要: | Transcriptomic adjustment plays an important role in Arabidopsis de-etiolation in response to environmental signals. The G-box cis-element is commonly present in the promoters of these genes that respond positively or negatively to the light signal. By using yeast two hybrid screening, a basic helix-loop-helix transcriptional regulator bHLH147 was identified to interact with a histone deacetylase HDA15. Analysis of the bhlh147 mutant revealed that bHLH147 may function in cell proliferation especially in stem elongation and root development, and act as a positive regulator in chlorophyll biosynthesis. Chromatin immunoprecipitation assays revealed that bHLH147 bind to the promoters of the chlorophyll biosynthesis genes GUN5, PSAE-1, PSBQ and LHCB2.2 to regulate plant photomorthogenesis. This study provided evidence indicating that bHLH147 plays versatile roles in control plant development, including stem elongation, root development, hypocotyl elongation, and chlorophyll biosynthesis in Arabidopsis. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16188 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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ntu-101-1.pdf 目前未授權公開取用 | 2.37 MB | Adobe PDF |
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