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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 吳克強 | |
dc.contributor.author | Ko-Ching Wang | en |
dc.contributor.author | 王科晶 | zh_TW |
dc.date.accessioned | 2021-06-07T18:04:21Z | - |
dc.date.copyright | 2012-08-28 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-07-30 | |
dc.identifier.citation | Alabadi, D., Gallego-Bartolome, J., Orlando, L., Garcia-Carcel, L., Rubio, V., Martinez, C., Frigerio, M., Iglesias-Pedraz, J.M., Espinosa, A., Deng, X.W., and Blazquez, M.A. (2007). Gibberellins modulate light signaling pathways to prevent Arabidopsis seedling de-etiolation in darkness. The Plant Journal 53, 324-335.
Alabadi, D. (2004). Gibberellins Repress Photomorphogenesis in Darkness. Plant Physiology 134, 1050-1057. Alabad′, D, and ′zquez, M.A.B. (2009). Molecular interactions between light and hormone signaling to control plant growth. Plant Mol Biol 69, 409-17 Achard, P. (2003). Ethylene Regulates Arabidopsis Development via the Modulation of DELLA Protein Growth Repressor Function. The Plant Cell 15, 2816-2825. Bernhardt, C. (2003). The bHLH genes GLABRA3 (GL3) andENHANCER OF GLABRA3 (EGL3) specify epidermal cell fate in the Arabidopsis root. Development 130, 6431-6439. Bouma, T. J., Nielsen, K.L., and Koutstaal, B. (2000). Sample preparation and scanning protocol for computerised analysis of root length and diameter. plant and soil 218, 185-196. Castillon, A., Shen, H., and Huq, E. (2007). Phytochrome Interacting Factors: central players in phytochrome-mediated light signaling networks. Trends in Plant Science 12, 514-521. Cheminant, S., Wild, M., Bouvier, F., Pelletier, S., Renou, J.P., Erhardt, M., Hayes, S., Terry, M.J., Genschik, P., and Achard, P. (2011). DELLAs Regulate Chlorophyll and Carotenoid Biosynthesis to Prevent Photooxidative Damage during Seedling Deetiolation in Arabidopsis. The Plant Cell 23, 1849-1860. Chory, J. (2010). Light signal transduction: an infinite spectrum of possibilities. The Plant Journal 61, 982-991. Chomczynski, P., and Sacchi, N. (1993). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. . Anal. Biochem 162, 156-159. Clough, S. J., and Bent, A.F. (1998). Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. The Plant Journal 16, 735-743. Durbak, A., H.Y.a.P.M. (2012). Hormone signaling in plant development. Current Opinion in Plant Biology 15, 92–96. Duek, P.D., and Fankhauser, C. (2005). bHLH class transcription factors take centre stage in phytochrome signalling. Trends in Plant Science 10, 51-54. Franco-Zorrilla, J.M., Solano, R., Trevisan, M., Pradervand, S., Xenarios, I., and Fankhauser, C. (2012). Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling. The Plant Journal 22, 1-13. Fu, X., and Harberd, N.P. (2003 ). Auxin promotes Arabidopsis root growth by modulating gibberellin response. Nature 421 740-743. Gendrel, A., Lippman, Z., Martienssen, R, and Colot, V. (2005). Profiling histone modification patterns in plants using genomic tiling microarrays. Nature Methods 2, 213 - 218. Gonzalez, A., Zhao, M., Leavitt, J.M., and Lloyd, A.M. (2008). Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings. The Plant Journal 53, 814-827. Hao, Y., Oh, E., Choi, G., Liang, Z., and Wang, Z.Y. (2012). Interactions between HLH and bHLH Factors Modulate Light-Regulated Plant Development. Molecular Plant 5, 688-697. Hornitschek, P., Kohnen, M.V., Lorrain, S., Rougemont, J., Ljung, K., Lopez-Vidriero, I., Ichihashi, Y., Horiguchi, G., Gleissberg, S., and Tsukaya, H. (2009). The bHLH Transcription Factor SPATULA Controls Final Leaf Size in Arabidopsis thaliana. Plant and Cell Physiology 51, 252-261. Kim, K., Shin, J., Lee, S.H., Kweon, H.S., Maloof, J.N., and Choi, G. (2011). Phytochromes inhibit hypocotyl negative gravitropism by regulating the development of endodermal amyloplasts through phytochrome-interacting factors. Proceedings of the National Academy of Sciences 108, 1729-1734. Kleine-Vehn, J., Langowski, L., Wisniewska, J., Dhonukshe, P., Brewer, P.B., and Friml, J. (2008). Cellular and Molecular Requirements for Polar PIN Targeting and Transcytosis in Plants. Molecular Plant 1, 1056-1066. Koprivova, A., Mugford, S.T., and Kopriva, S. (2010). Arabidopsis root growth dependence on glutathione is linked to auxin transport Plant Cell Rep 29, 1157-1167. Leivar, P., and Quail, P.H. (2011). PIFs: pivotal components in a cellular signaling hub. Trends in Plant Science 16, 19-28. Lu, Q., Tang, X., Tian, G., Wang, F., Liu, K., Nguyen, V., Kohalmi, S.E., Keller, W.A., Tsang, E.W., Harada, J.J., Rothstein, S.J., and Cui, Y. (2010). rabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Plant Journal 61, 259-270. Moon, J., Zhu, L., Shen, H., and Huq, E. (2008). PIF1 directly and indirectly regulates chlorophyll biosynthesis to optimize the greening process in Arabidopsis. Proceedings of the National Academy of Sciences 105, 9433-9438. Nagatani, A., Reed, J.W., and Chory, J. (1993). lsolation and lnitial Characterization of Arabidopsis Mutants That Are Deficient in Phytochrome A. Plant Physiology 102, 269-277. Oh, E. (2004). PIL5, a Phytochrome-Interacting Basic Helix-Loop-Helix Protein, Is a Key Negative Regulator of Seed Germination in Arabidopsis thaliana. The Plant Cell 16, 3045-3058. Oh, E., Yamaguchi, S., Hu, J., Yusuke, J., Jung, B., Paik, I., Lee, H.S., Sun, T.p., Kamiya, Y., and Choi, G. (2007). PIL5, a Phytochrome-Interacting bHLH Protein, Regulates Gibberellin Responsiveness by Binding Directly to the GAI and RGA Promoters in Arabidopsis Seeds. The Plant Cell 19, 1192-1208. Overvoorde, P., Fukaki, H., and Beeckman, T. (2010). Auxin Control of Root Development. Cold Spring Harbor Perspectives in Biology 2, a001537-a001537. Park, J., Lee, N., Kim, W., Lim, S., and Choi, G. (2011). ABI3 and PIL5 Collaboratively Activate the Expression of SOMNUS by Directly Binding to Its Promoter in Imbibed Arabidopsis Seeds. The Plant Cell 23, 1404-1415. Penfield, S., Josse, E.-M., Kannangara, R., Gilday, A.D., Halliday, K.J., and Graham, I.A. (2005). Cold and Light Control Seed Germination through the bHLH Transcription Factor SPATULA. Current Biology 15, 1998-2006. Richards, E., Reichardt, M., and Rogers, S. (1994). Preparation of Genomic DNA from Plant Tissue. Current Protocols in Molecular Biology 2.3.1-2.3.7. Reed, J. W., Nagpal, P., Poole, D.S., Furuya, M., and Chory, J. (1993). Mutations in the Gene for the Red/Far-Red Light Receptor Phytochrome B Alter Cell Elongation and Physiological Responses throughout Arabidopsis Development. The Plant Cell 5, 147-157. Shin, J., Park, E., and Choi, G. (2007). PIF3 regulates anthocyanin biosynthesis in an HY5-dependent manner with both factors directly binding anthocyanin biosynthetic gene promoters in Arabidopsis. The Plant Journal 49, 981-994. Shimizu, T., Toumoto, A., Ihara, K., Shimizu, M., Kyogoku, Y., Ogawa, N., Oshima, Y., and Hakoshima, T. (1997). Crystal structure of PHO4 bHLH domain–DNA complex: flanking base recognition. The EMBO Journal 16, 4689-4697. Spivak-Kroizman, T., Lemmon, M. A., Dikic, f., Ladbury, J. E., Pinchasi, D., Huang, J., Jaye, M., Crumley, G., Schlessinger, J., and Lax, I. (1994). Heparin-induced oligomerization of FGF molecules is responsible for FGF receptor dimerization, activation, and cellproliferation. Cell 79, 1015-1024. Stephenson, P.G., Fankhauser, C., and Terry, M.J. (2009). PIF3 is a repressor of chloroplast development. Proceedings of the National Academy of Sciences 106, 7654-7659. Sun, J., , L.Q., and , Y.L., Jinfang Chu, Chuanyou Li. (2012). PIF4–Mediated Activation of YUCCA8 Expression Integrates Temperature into the Auxin Pathway in Regulating Arabidopsis Hypocotyl Growth. PLoS Genet 8 e1002594. Tanimoto, E. (2005). Regulation of Root Growth by Plant Hormones—Roles for Auxin and Gibberellin. Critical Reviews in Plant Sciences 24, 249-265. Toledo-Ortiz, G. (2003). The Arabidopsis Basic/Helix-Loop-Helix Transcription Factor Family. The Plant Cell 15, 1749-1770. Ubeda-Toma ′ s, S., Beemster, G.T.S., and Bennett, M.J. (2012). Hormonal regulation of root growth integrating local activities into global behaviour. Trends in Plant Science 17, 326-331. Ubeda-Toma ′s, S., Federici, F., Casimiro, I., Beemster G.T.S., Bhalerao, R., Swarup, R., Doerner ,P., Haseloff ,J., and Bennett, M.J. ( 2009). Gibberellin Signaling in the Endodermis Controls Arabidopsis Root Meristem Size. Current Biology 19, 1194-1199. Ugartechea-Chirino, Y., Swarup, R., Swarup, K., Peret, B., Whitworth, M., Bennett, M., Bougourd, S. (2009). The AUX1 LAX family of auxin influx carriers is required for the establishment of embryonic root cell organization in Arabidopsis thaliana. Annals of Botany105, 277-289. Wang, Y. (2008). Progress of studies on bHLH transcription factor families. Hereditas (Beijing) 30, 821-830. Yamaguchi, and Shinjiro. (2008). Gibberellin Metabolism and its Regulation. Annual Review of Plant Biology 59, 225-251. Yoo, S.D., Cho, Y.-H., and Sheen, J. (2007). Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nature Protocols 2, 1565-1572. Zhang, L.Y., Bai, M.Y., Wu, J., Zhu, J.Y., Wang, H., Zhang, Z., Wang, W., Sun, Y., Zhao, J., Sun, X., Yang, H., Xu, Y., Kim, S.H., Fujioka, S., Lin, W.H., Chong, K., Lu, T., and Wang, Z.Y. (2009). Antagonistic HLH/bHLH Transcription Factors Mediate Brassinosteroid Regulation of Cell Elongation and Plant Development in Rice and Arabidopsis. The Plant Cell 21, 3767-3780. Zhang, W. (2006). Regulation of Arabidopsis tapetum development and function by DYSFUNCTIONAL TAPETUM1 (DYT1) encoding a putative bHLH transcription factor. Development 133, 3085-3095. Zhong-Lin Zhang, Mikihiro Ogawa, Christine M. Fleet, Rodolfo Zentella, Jianhong Hu, Jung-Ok Heo, Jun Lim, Yuji Kamiya, Shinjiro Yamaguchi, and Sun, T.-p. (2011). SCARECROW-LIKE 3 promotes gibberellin signaling by antagonizing master growth repressor DELLA in Arabidopsis. Proceedings of the National Academy of Sciences 108, 2160-2165. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16188 | - |
dc.description.abstract | Transcriptomic adjustment plays an important role in Arabidopsis de-etiolation in response to environmental signals. The G-box cis-element is commonly present in the promoters of these genes that respond positively or negatively to the light signal. By using yeast two hybrid screening, a basic helix-loop-helix transcriptional regulator bHLH147 was identified to interact with a histone deacetylase HDA15. Analysis of the bhlh147 mutant revealed that bHLH147 may function in cell proliferation especially in stem elongation and root development, and act as a positive regulator in chlorophyll biosynthesis. Chromatin immunoprecipitation assays revealed that bHLH147 bind to the promoters of the chlorophyll biosynthesis genes GUN5, PSAE-1, PSBQ and LHCB2.2 to regulate plant photomorthogenesis. This study provided evidence indicating that bHLH147 plays versatile roles in control plant development, including stem elongation, root development, hypocotyl elongation, and chlorophyll biosynthesis in Arabidopsis. | en |
dc.description.provenance | Made available in DSpace on 2021-06-07T18:04:21Z (GMT). No. of bitstreams: 1 ntu-101-R99b42006-1.pdf: 2424120 bytes, checksum: b7fd86ba41820987c78a08de352a9072 (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 致謝 I
中文摘要 II Abstract III List of Abbreviation IV List of Figures VIII List of Tables IX List of Supplementary Figures X Introduction 1 Basic/helix-loop-helix (bHLH) proteins 1 Role of bHLHs in light signaling 2 PIFs and pytohormone signaling 6 Materials and Methods 10 Plant materials 10 Quick DNA Extration 10 RNA isolation 11 RT-PCR analysis 13 Real-time RT-PCR analysis 13 Bimolecular Fluorescence Complementation (BiFC) Assay 14 Generating transgenic plants overexpressing AtbHLH147 17 Seed germination in Petri dishes 19 Measurement of germination rates and root lengths 19 Phenotype observation of hypocotyl lengths 20 Root staining and meristem cell count 20 Protochlorophyllide determination 20 Chromatin immunoprecipitation (ChIP) assay 21 Results 28 Phylogenic analysis and sequence comparison of bHLH proteins 28 Expression and subcellular localization of bHLH147 28 bHLH147 interacted with HDA15 and DELLA proteins. 29 Identification of a bHLH147 T-DNA insertion mutant 30 Generation of transgenic lines overexpressing bHLH147 30 Phenotypes of the bhlh147-1 mutant 31 bHLH147 affects gene expression involved in GA biosynthesis. 31 bHLH147 affects auxin biosynthesis in roots. 32 bHLH147 is involved in hypocotyl elongation and chlorophyll biosynthesis 34 Discussions 37 bHLH147 functions in GA-mediated stem elongation 37 bHLH147 regulates cell proliferation in roots 38 bHLH147 regulates hypocotyl elongation 40 bHLH147 positively regulates chlorophyll biosynthesis and photosynthesis in etiolated seedlings 41 Reference 43 Tables 51 Figures 55 | |
dc.language.iso | en | |
dc.title | 阿拉伯芥AtbHLH147基因功能性分析 | zh_TW |
dc.title | Functional analysis of AtbHLH147 in Arabidopsis | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 金洛仁,鄭石通,林讚標,鄭貽生 | |
dc.subject.keyword | 阿拉伯芥,bHLH蛋白,生長素,吉貝素,葉綠素,光型態發生, | zh_TW |
dc.subject.keyword | Arabidopsis,bHLH147,Auxin,Gibberellin,Chlorophyll,Photomorphogenesis, | en |
dc.relation.page | 70 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2012-07-30 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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