請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16129
標題: | 惡性神經纖維鞘瘤之光動力治療效果 Effects of photodynamic therapy on Malignant Peripheral Nerve Sheath Tumor |
作者: | Che-Hao Wang 王哲豪 |
指導教授: | 陳進庭(Chin-Tin Chen) |
關鍵字: | 惡性神經纖維鞘瘤,五氨基酮戊酸光動力療法,雙效微脂體, malignant peripheral nerve sheath tumor,5-Aminolevulinic acid-mediated photodynamic therapy,dual-effect liposome, |
出版年 : | 2012 |
學位: | 碩士 |
摘要: | 惡性神經纖維鞘瘤 (Malignant peripheral nerve sheath tumor cell,MPNST) 為源自周邊神經細胞的腫瘤,主要病徵為病患表皮上會增生許多大小不一的神經纖維瘤 (neurofibroma) ,且腫瘤細胞容易轉移到身體其他部位。腫瘤除了會造成生理上的病痛之外,外觀上的不雅也嚴重影響患者的心理發展。MPNST細胞主要會沿著神經脈絡生長並與正常細胞緊密交纏,導致以外科手術切除腫瘤的困難度增高,且其對化療及放射線治療具有抗性,使得治療效果不佳。因此,本研究的目的為找尋更有效率治療MPNST的方法,希望將來能在臨床治療上提供幫助。在目前的臨床研究上,五氨基酮戊酸光動力療法 (5-Aminolevulinic acid photodynamic therapy, ALA-PDT) 因具有光感物質選擇性的累積於腫瘤之特質,藉由局部照光所產生的單態氧及自由基可專一地對腫瘤細胞造成破壞,因此本研究針對MPNST細胞進行光照治療處理。結果顯示,ALA-PDT可有效的毒殺MPNST細胞,且處理後存活下來之細胞的轉移能力顯著下降。另有文獻指出,Neurofibromin type1 (NF1) 基因的缺失容易導致良性的周邊神經瘤惡化,此外NF1基因缺失的MPNST細胞也被認為是較惡性的腫瘤,且大部分具有較快的生長速度和轉移能力。而本研究發現,相較於NF1-wild type MPNST細胞,ALA-PDT對NF1基因缺失的MPNST細胞具有更強的毒殺效果;並發現其原因為在NF1基因缺失的MPNST細胞內,由ALA轉換成之光感物質PpIX累積量相對較高的緣故。另外,本實驗室先前研究利用微脂體將化療藥物及光感物質包覆其內,以此種結合光動力治療與化學治療效應之腫瘤治療模式可以更加提升對癌症治療之效益。為了在將來能更進一步將光動力療法應用於臨床治療MPNST;本研究進行活體動物實驗 (in vivo study)發現施打雙效微脂體並進行光照處理的小鼠之腫瘤細胞生長將受到抑制、甚至消失,顯示光動力療法的確具有治療MPNST的潛力,並且可於將來應用於臨床治療。 Malignant peripheral nerve sheath tumor (MPNST) is a kind of soft tissue sarcomas derived from peripheral nerves and has poor prognosis. MPNSTs appear in varied sizes and tend to metastasize to other parts of the body. In addition to the physical conditions, marked cosmetic disfigurements also cause psychological traumas. Because the tumors are resistant to chemical and radical treatments, no effective therapies are available except for surgical resection. 5-Aminolevulinic acid-mediated photodynamic therapy (ALA-PDT), which involved the photochemical reactions based on the interaction of photosensitizing agents and light energy, has become a modality in anti-tumor therapy. Since the selective accumulation of ALA-derived protoporpyrin IX (PpIX) in tumor cells, topical illumination can specifically induce oxidative stress followed by cell death to tumors. In this study, significant cytotoxicity and reduced migration ability were found in MPNST cells after ALA-PDT. Besides, we found out that NF1-mutation MPNST cells, are more sensitive to PDT compared to NF1 wild type MPNST cells. We also treated MPNST cells with dual-effect liposome and find good therapeutic effects in vitro and in vivo. Our studies suggest that ALA-PDT and dual-effect liposome have potential therapeutic effects for the treatment of MPNST. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/16129 |
全文授權: | 未授權 |
顯示於系所單位: | 生化科技學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-101-1.pdf 目前未授權公開取用 | 3.73 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。