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標題: | HC植物萃取物對於乳癌及前列腺癌潛在抗癌效應 Potential anti-tumor effect of HC plant extract on breast and prostate cancer |
作者: | Chi-Han Wu 吳奇翰 |
指導教授: | 蕭培文 |
共同指導教授: | 鄭石通 |
關鍵字: | HC植物,抗發炎,原位同源及異源性移植,乳癌,前列腺癌, HC,anti-inflammation,orthotopic allograft and xenograft transplants,breast cancer,prostate cancer, |
出版年 : | 2013 |
學位: | 碩士 |
摘要: | 慢性發炎可促發許多不同種類的癌症生長。因此,減緩發炎成為抗癌治療上的重要契機。多篇報導指出中草藥之一的HC 植物,具備抗發炎、抗病毒、抗細菌及抗白血病的生物特性。然而,利用HC治療其他癌症的發生鮮少有文獻提及。本篇論文中,我們同時在細胞層次及動物活體內研究該草藥在治療乳癌及前列腺癌的效果。藉由以水及甲醇為移動相建立的矽膠管柱層析法,我們將HC酒精粗萃物分為16個區段分別收集。由本實驗室先前建立的報導基因細胞模式發現,其中的區段6,7,8號可在前列腺癌細胞中,調控受雄性素接受器控制前列腺特異性抗原之下游報導基因的轉錄作用,而且該調控效果亦會隨著藥物劑量增加而愈趨明顯;進一步由化學鑑定方式,確定F7-3正是7號區段中主要的活性成份。我們利用噬菌斑減少分析法發現區段8及9號可抑制流行性感冒病毒的複製,而10號區段也具備些微的抑制效果。即便在100 μg/ml的濃度,分別以6, 7, 8, 9, 10號區段給予不同種類的乳癌及前列腺癌細胞處理24小時,仍無法顯著地降低癌細胞的存活率,但8號區段卻能有效地抑制這些癌細胞的侵犯能力。本實驗室亦利用實驗動物原位植入小鼠同源乳癌及異源前列腺癌細胞的活體模式分析HC酒精萃取物之配方的藥效。該草藥萃取物可減緩原位癌的生長、抑制癌細胞體內轉移,且未有明顯的毒性。利用此一臨床前實驗研究法證明,HC可作為化學治療或輔助療法上的選擇。 Chronic inflammation can augment tumor development in various types of cancers. Reduction of inflammation, is therefore, an important anticancer therapeutic opportunity. It is reported that HC, a traditional Chinese herbal medicine, has many biological properties such as anti- inflammation, antiviral, antibacterial and anti-leukemic activities. The application of this herb to other cancers, however, is rarely proposed. In this study, we applied this herbal drug to breast and prostate cancers both in vitro and in vivo. The HC ethanolic crude extract was fractionated by silica gel column chromatography using a H2O and EtOH developing system, resulting in the collection of HC fractions 1~16. We found that HC ethanolic extract fractions 6,7, and 8 could modulate the androgen receptor (AR) activation of transcription from prostate-specific antigen (PSA) promoter within prostate cancer cells in a dose-dependent manner and further identified F7-3 within F7 was the major constituent that contributed to its activity. We also found that HC F8 and F9 could inhibit replication of influenza virus via plaque reduction assay (data not shown). F10 also had very slight effect of this (data not shown). Even though treatment with HC F6, 7, 8, 9, and 10 separately at concentrations below 100 ug/ml for 24 hours showed no significant decrease in the survival rate of either types of prostate cancer cell lines, HC F8 could inhibit the invasiveness of these cancer cells efficiently. The in vivo efficacy of oral administration of a standardized extract of HC were analyzed in animals bearing orthotopic breast cancer allograft and prostate cancer xenograft transplants. The herbal extract retarded the tumor growth without observable toxicity. This preclinical study thus provides strong evidence that HC is a good candidate for chemoprevention or complementary medicine. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/15577 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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