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Title: | 利用表面增強拉曼光譜法基質在不受光譜干擾的區域對分子進行研究 Study of Molecules in Optical Interference-Free Spectra Region with Fabricated SERS Substrate |
Authors: | Zi-Yao Hong 洪子堯 |
Advisor: | 江建文(Kien Voon Kong) |
Keyword: | 不受生物分子訊號干擾的光譜區域,表面增強拉曼光譜基質, Spectral region free from biomolecules signals,Surface Enhanced Raman Spectroscopy(SERS), |
Publication Year : | 2018 |
Degree: | 碩士 |
Abstract: | 在1800 cm-1到2500 cm-1的區域是比較不會受到來自醣類,蛋白質等生物分子干擾的區域,然而許多具有參鍵的分子在這個位置則是有明顯的訊號,因此本論文選擇幾個具有CC三鍵或是CN三鍵的分子來做測試,分別為benzonitrile, 4-iodophthalonitrile, (triphenylsilyl)acetylene, 1,2-bis(triphenylsilyl)acetylene,並且測量他們的訊號是否在這個區域,並且在確認了他們在這個區域有SERS(Surface Enhancement Raman Spectroscopy,SERS)訊號之後,更進一步地把溶液進行稀釋,得到強度對溶液的關係圖,這些圖也符合常見的SERS訊號對濃度作圖所得到的吸附曲線。並且也透過測試他們在鍍金SERS基質上的作用力來評估他們做為發訊分子的可行性。
除了找尋在1800 cm-1到2500 cm-1波長範圍有訊號的適當的分子以外,具有靈敏度,再現性等特性的SERS基質在感測應用上也是很重要的一環,因此本論文也試著以無遮罩反應式離子蝕刻(maskless reactive ion etching, maskless RIE)製程來製造SERS基質,因為最終的SERS基質是先透過RIE製程,接著再用電子束蒸鍍的方式鍍上金,因此調整RIE參數像是蝕刻氣體流速比例,蝕刻功率對於製造出可以產生SERS訊號的奈米結構是相當關鍵的,並且所產生的奈米結構可以透過掃描式電子顯微鏡 (scanning electron microscopy, SEM) 影像來確認。最後,我們從自製晶片對於分子訊號的測試顯示了我們成功地製造出具有SERS活性的基質。 The spectral region in 1800~2500 cm-1 is well-known for the absence of interference signal from biomolecules such as proteins or carbohydrates. However, molecules with triple bond have signal in this region. So we choose molecules containing CC and CN triple bond that is, benzonitrile, 4-iodophthalonitrile, (triphenylsilyl)acetylene, 1,2-bis(triphenylsilyl)acetylene to test their signal intensity and position. Their signals on the gold chip upon serial dilution also behave like typical adsorption isotherm. Affinity on gold coated SERS substrate is also tested to evaluate the feasibility of using them as reporter molecules. In addition to looking for appropriate molecules with signal in the 1800~2500 cm-1, SERS substrate with good sensitivity, reproducibility is also a desirable goal for sensing applications. We try to fabricate SERS substrate by a maskless reactive ion etching (RIE) process. Because SERS substrate is fabricated through the RIE procedure followed by e-beam evaporation of Au, it is crucial to adjust the RIE parameters (etching gas ratio, etching power) to get a nanostructure suitable for SERS signal generation, and the fabricated nanostructure could be identified through scanning electron microscopy images of fabricated substrates. Finally, SERS signals of the molecules on the in-house fabricated substrates demonstrate that we successfully fabricate a SERS substrate through a simple maskless process. |
URI: | http://tdr.lib.ntu.edu.tw/handle/123456789/1138 |
DOI: | 10.6342/NTU201802323 |
Fulltext Rights: | 同意授權(全球公開) |
Appears in Collections: | 化學系 |
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ntu-107-1.pdf | 2.24 MB | Adobe PDF | View/Open |
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