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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 劉懷勝(Hwai-Shen Liu) | |
| dc.contributor.author | Hui-Min Hsieh | en |
| dc.contributor.author | 謝惠敏 | zh_TW |
| dc.date.accessioned | 2021-05-20T21:00:58Z | - |
| dc.date.available | 2016-07-26 | |
| dc.date.available | 2021-05-20T21:00:58Z | - |
| dc.date.copyright | 2011-07-26 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-07-20 | |
| dc.identifier.citation | Abadias, M., A. Benabarre, N. Teixido, J. Usall & I. Vinas (2001) Effect of freeze drying and protectants on viability of the biocontrol yeast Candida sake. International Journal of Food Microbiology, 65, 173-182.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10087 | - |
| dc.description.abstract | 本研究目的主要在於探討如何提升及應用R. erythropolis NTU-1的細胞聚集現象，使長鏈烷類在短時間內能大量移除。
研究結果發現，在培養錐形瓶中加入篩網可幫助迴旋式培養下NTU-1與正十六烷的接觸機會及形式，因而NTU-1在44小時內即能形成結塊，配合物理撈除的方式可將2000 ppmv正十六烷移除掉95%以上。 利用冷凍乾燥的方式來保存以NB培養的NTU-1細胞，實驗結果發現使用麥芽糖溶液作為冷凍乾燥保護劑，並存放於－20℃的環境下，乾燥的NTU-1在經過30天存放後仍具有很高的存活率且其生理特性並不會受到影響。同樣利用冷凍乾燥的方式來乾燥以正十六烷培養而得的NTU-1細菌結塊，然而實驗結果顯示乾燥後的NTU-1結塊活性很低，無法再降解烷類，但其再形成結塊包覆烷類的能力並不會受到影響，1 ~ 2小時內即能再次形成聚集體，第12小時以物理撈除的方式可移除80 ~ 90%濃度為2000 ppmv的正十六烷。 接著以烘乾的方式取代成本高及費時的冷凍乾燥法來乾燥NTU-1結塊。實驗中選擇烘乾溫度為80℃，其乾燥所需時間約15小時且乾燥後的NTU-1結塊具有良好形成聚集包覆烷類的效果。這個部分換算出利用1g/L的NTU-1結塊密度來處理10000 ppmv的正十六烷較佳。另外，若以兩段式添加乾燥NTU-1結塊的方法，可使正十六烷的移除效率在12小時內由90%提升至將近100%。 乾燥後NTU-1結塊再形成聚集將烷類包覆的原因與其細胞表面疏水性及完整性有關，此兩項特性需同時存在，乾燥NTU-1結塊才能貼附烷類且以堆疊的方式形成大顆粒結塊。 由此可知，將NTU-1結塊冷凍乾燥或烘乾後保存，利用乾燥NTU-1結塊的高度包覆烷類能力，配合物理撈除的方式，使烷類能在12小時內大量移除，此項成果無疑的為將來石油污染物移除提供了一個相當具競爭性及發展性的方法。 | zh_TW |
| dc.description.abstract | Rhodococcus erythropolis NTU-1 is a strain which can not only degrade hydrocarbons but also traps alkanes in biofloccules during bioremediation process. In this study, we focused on how to accelerate biofloccules formation and to apply NTU-1 biofloccules for n-hexadecane (n-C16) removal.
Results showed that sieves in Erlenmeyer flasks increased the n-C16 agitation efficiency under orbital shaking and assisted NTU-1 to utilize n-C16. NTU-1 formed biofloccules and trapped most residual n-C16 within 44 hr. At this time, more than 95% n-C16 was removed. NTU-1 cells (cultured by Nutrient Broth) by freeze-drying preserved most viability with maltose as the protectant. However, freeze-dried NTU-1 biofloccules (cultured by n-C16) resulted in low cell viability. Dried NTU-1 biofloccules did not degrade n-C16 but they still re-formed biofloccules and re-trapped most of n-C16. With these freeze-dried biofloccules, n-C16 removal efficiency achieved 80 ~ 90% within 12 hr. Heat-dried biofloccules were further evaluated because of its simplicity in comparison with freeze-drying method. It proved a good method and we found 1g/L dried cell vs. 10000 ppmv n-C16 was an optimal ratio. Moreover, with two-step addition of dried NTU-1 biofloccules, near 100% of n-C16 was removed in 12 hr. The mechanism of re-forming biofloccules was found to relate to the cell hydrophobicity and integrity. With these two characteristics, dried NTU-1 biofloccules adhered to n-C16, and n-C16 worked as the linker to form cell pellets in a short time. These dried biofloccules provided a feasible and potential method for remediation of hydrocarbon pollutants. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-20T21:00:58Z (GMT). No. of bitstreams: 1 ntu-100-R98524034-1.pdf: 9627761 bytes, checksum: 2828efb151749663b733aed6ee47fed0 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 目錄
致謝 I 中文摘要 II Abstract III 目錄 IV 圖目錄 VII 表目錄 XIV 照片目錄 XV 第一章 緖論 1 1.1 前言 1 1.2 研究目的及論文綱要 2 第二章 文獻回顧 3 2.1 石油碳氫化合物簡介及其對環境和人類之影響 3 2.2 處理石油碳氫化合物污染之方法 5 2.3 生物處理石油碳氫化合物 9 2.3.1 生物復育簡介 9 2.3.2 微生物攝取碳氫化合物之模式 14 2.3.3 微生物分解碳氫化合物之方式 20 2.4 微生物降解碳氫化合物之代謝途徑 23 2.4.1 直鏈烷之氧化機制 23 2.4.2 支鏈烷之氧化機制 26 2.4.3 烯烴類及環烷類之氧化機制 31 2.5 實驗菌株Rhodococcus erythropolis之介紹 34 2.5.1 Rhodococcus菌屬簡介 34 2.5.2 Rhodococcus erythropolis之特性及應用 37 2.6 微生物之細胞聚集現象 42 2.7 微生物乾燥技術及應用 45 2.7.1 冷凍乾燥 (Freeze-drying) 45 2.7.1.1 冷凍乾燥對菌株的影響 46 2.7.1.2 冷凍乾燥時保護劑的添加 48 2.7.1.3 冷凍乾燥後菌株貯存之安定性 51 2.7.2 烘乾之原理及應用 52 第三章 實驗材料與方法 53 3.1 實驗菌株 53 3.2 培養基組成與配製 55 3.2.1 液態礦物培養基 55 3.2.2 菌株保存培養基 58 3.2.3 菌株活化培養基 59 3.2.4 計數平板培養基 59 3.3 實驗方法 60 3.3.1 菌株的活化及生長曲線 60 3.3.2 礦物培養基菌液製作 61 3.3.3 正十六烷之生物降解與生物復育實驗 62 3.3.4 以NB培養的NTU-1進行冷凍乾燥及其存活率測試 65 3.3.5 以正十六烷培養的NTU-1細菌結塊進行冷凍乾燥 67 3.3.6 烘乾以正十六烷培養的NTU-1細菌結塊 68 3.3.7 乾燥後NTU-1細菌結塊對於正十六烷的包覆與移除 69 3.3.8 不同條件乾燥下NTU-1細菌結塊之細胞表面疏水性測定 70 3.4 實驗藥品與器材 71 3.4.1 實驗藥品 71 3.4.2 實驗儀器 72 第四章 實驗結果與討論 73 4.1 不同搖晃培養對於NTU-1生物降解及包覆正十六烷能力的影響及改進方法 74 4.1.1 比較往復式及迴旋式培養下NUT-1對正十六烷生物復育的效果 74 4.1.2 迴旋式培養下錐形瓶中加入篩網時正十六烷生物復育的效果 81 4.1.2.1 篩網孔徑大小為10 mesh 81 4.1.2.2 篩網孔徑大小為20 mesh 89 4.1.2.3 不同孔徑大小但相同折角 (45度) 篩網之比較 95 4.1.3 討論 98 4.2 以NB培養的NTU-1冷凍乾燥後細胞存活率及降解包覆能力探討 101 4.2.1 利用NB培養的NTU-1經過冷凍乾燥後之存活率探討 101 4.2.2 以NB培養的NTU-1經過冷凍乾燥後對於正十六烷降解及包覆能力探討 109 4.2.3 討論 113 4.3 利用正十六烷培養的NTU-1細菌結塊冷凍乾燥後其存活率及降解包覆能力探討 114 4.3.1 NTU-1細菌結塊降解及包覆正十六烷的能力 (未經冷凍乾燥) 114 4.3.2 NTU-1細菌結塊在冷凍乾燥後降解及包覆正十六烷的能力 120 4.3.3 超音波震碎NTU-1結塊並進行冷凍乾燥後之應用 144 4.3.4 討論 146 4.4 利用正十六烷培養的NTU-1細菌結塊經烘乾後再聚集包覆烷類之能力探討 149 4.4.1 烘乾NTU-1結塊時溫度與時間的選擇 149 4.4.2 不同條件乾燥下NTU-1結塊表面疏水性測定 156 4.4.3 烘乾後的NTU-1結塊存放溫度與時間之探討 159 4.4.4 烘乾後NTU-1結塊於培養過程12小時間的細胞聚集情形 162 4.4.5 以不同量之乾燥NTU-1結塊處理2000 ppmv正十六烷 166 4.4.6 以固定量之乾燥NTU-1結塊處理不同濃度的正十六烷 171 4.4.7 提高乾燥NTU-1結塊移除正十六烷效率的方法 177 4.4.8 乾燥NTU-1結塊再形成聚集體包覆正十六烷之機制 181 4.4.9 討論 184 第五章 結論 187 參考文獻 190 附錄1 NB培養的NTU-1及正十六烷培養的NTU-1結塊經冷凍乾燥後的結合應用 201 附錄2 不同條件乾燥後NTU-1結塊之細胞粒徑大小 207 | |
| dc.language.iso | zh-TW | |
| dc.title | 利用 Rhodococcus erythropolis NTU-1 細胞聚集現象移除正十六烷 | zh_TW |
| dc.title | Rhodococcus erythropolis NTU-1 Biofloccules for
n-Hexadecane Removal | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 許駿發(Chun-Fa Hsu),李振綱(Cheng-Kang Lee),王孟菊(Meng-Jiy Wang) | |
| dc.subject.keyword | 細胞聚集現象,正十六烷,冷凍乾燥,烘乾, | zh_TW |
| dc.subject.keyword | Rhodococcus erythropolis NTU-1,biofloccules,freeze-drying,heat-drying,n-hexadecane removal, | en |
| dc.relation.page | 210 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2011-07-20 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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