請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10054
標題: | 水稻側根與根毛形成之研究 Studies on the Formation of Lateral Roots and Root Hairs in Rice |
作者: | Yi-Hsuan Chen 陳怡萱 |
指導教授: | 高景輝(Ching Huei Kao) |
關鍵字: | 生長素,一氧化氮,側根,根毛,水稻,鈣,HO,apocynin, auxin,nitric oxide,lateral root,root hair,rice,calcium,HO,apocynin, |
出版年 : | 2011 |
學位: | 碩士 |
摘要: | 本論文以水稻品種台中在來一號 (Oryza sativa L. cv. Taichung Native 1, TN1)為材料,探討生長素與一氧化氮對黃化幼苗側根與根毛之形成是否需要Ca2+與heme oxygenase (HO)之參與,以及apocynin對水稻黃化幼苗側根形成之影響。
Sodium nitroprusside (SNP,一氧化氮釋放劑)、indole-3-butyric acid (IBA,植物體中存在之生長素)與hemin (Hm,HO之誘導劑)處理皆會誘導水稻側根與根毛之形成。SNP與IBA所誘導之一氧化氮形成以及側根與根毛形成可由一氧化氮清除劑2- (4-carboxy- phenyl)- 4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide (cPTIO)所抑制,而Hm處理不影響一氧化氮之形成,且其對側根與根毛形成之作用也不受cPTIO影響,nitrate reductase (NR)抑制劑tungstate會抑制IBA所誘導之一氧化氮形成以及側根與根毛之形成,顯示IBA是透過NR的作用產生一氧化氮進而影響側根與根毛之形成。Ca2+之螯合物、通道阻礙劑、CaM拮抗劑與IP3合成抑制劑皆可明顯抑制SNP與IBA所誘導之側根與根毛形成,而不影響SNP與IBA所誘導之一氧化氮形成,顯示SNP與IBA之作用需要Ca2+之參與,且Ca2+位於一氧化氮與IBA作用之下游。 SNP、IBA與Hm處理皆會使HO的活性提升,Zn protoporphyrin IX (ZnPPIX,HO抑制劑)與hemoglobin (Hb,一氧化碳與一氧化氮之清除劑)皆能有效抑制SNP、IBA與Hm誘導之側根與根毛形成及HO的活性提升。而HO催化反應之產物biliverdin IXα (BV) 也可有效地誘導側根與根毛形成,這些結果說明SNP、IBA與Hm所誘導之水稻黃化幼苗側根與根毛之形成需要透過HO的參與。 Apocynin會誘導水稻側根形成與H2O2合成,而diphenylene iodonium (DPI,NADPH oxidase抑制劑)可抑制其作用,顯示apocynin經由H2O2之作用調控側根形成。 In this thesis, rice (Oryza sativa L. cv. Taichung Native 1, TN1, an Indica type) seedlings were used to investigate the involvement of Ca2+ and heme oxygenase (HO) in auxin- and nitric oxide (NO)-induced formation of lateral roots (LRs) and root hairs (RHs) and the effect of apocynin on LR formation. Application of sodium nitroprusside (SNP; a NO donor), indole-3-butyric acid (IBA; a naturally occurring auxin), or hemin (Hm; a HO inducer) to rice seedlings induced the formation of LRs and RHs. LR and RH formation and NO production induced by SNP and IBA were prevented by the specific NO scavenger 2-(4-carboxyphenyl)-4,4,5,5- tetramethyl-imidazoline-1-oxyl-3-oxide (cPTIO). Hm had no effect on NO production. Hm-induced formation of LRs and RHs could not be blocked by cPTIO. Nitrate reductase (NR) inhibitor sodium tungstate completely inhibited IBA-induced LR and RH formations and NO production. Clearly the effect of IBA is attributed by NO released, and the NO generation in response to IBA might mainly involve NR activity. The effects of Ca2+ chelators, Ca2+-channel inhibitors, and calmodulin antagonists were effective in reducing the action of SNP and IBA. However, Ca2+ chelators and Ca2+- channel inhibitors had no effect on SNP- and IBA-induced NO generation. It is concluded that Ca2+ is involved in SNP- and IBA-induced LR and RH formations, and is acting downstream of NO and IBA. Treatment of rice seedlings with SNP, IBA, and Hm resulted in an enhancement of HO activity. Zn protoporphyrin IX (ZnPPIX; a HO inhibitor) and hemoglobin (Hb; a CO/NO scavenger) reduced the LR and RH formation and the enhancement of HO activity induced by SNP, IBA, and Hm. The product of HO catalyzed reaction, biliverdin IXα (BV), was also able to induce LR and RH formation and enhance HO activity. These data suggested that HO is involved in SNP-, IBA- and Hm-induced LR and RH formation. Apocynin was able to induce LR formation and the generation of H2O2, which could be blocked by diphenylene iodonium (a NADPH oxidase inhibitor). These results indicate that the apocynin-induced LR formation is related to H2O2. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/10054 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 農藝學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-100-1.pdf | 9.19 MB | Adobe PDF | 檢視/開啟 |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。