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標題: | 超音波對肌肉再生之探討 Investigation of Ultrasound Stimulation on Muscle Regeneration |
作者: | 林威廷 Wei-Ting Lin |
指導教授: | 王兆麟 Jaw-Lin Wang |
關鍵字: | 低能量超音波,壓電刺激,肌肉萎縮,C2C12肌母細胞,初級纖毛,分化, Low-intensity ultrasound,Piezoelectric stimulation,Muscle atrophy,C2C12 Myoblast,Primary cilia,Differentiation, |
出版年 : | 2023 |
學位: | 碩士 |
摘要: | 低能量超音波已被證實能夠促進C2C12的增生與分化。壓電效應則被證實能透過初級纖毛來重新排列軟骨細胞,且初級纖毛在骨骼肌肉分化中扮演重要的角色。然而,超音波與壓電對於治療肌肉萎縮的應用仍未明朗,故本研究旨在探討超音波與壓電刺激在治療肌肉萎縮方面的可行性。
我們選擇C2C12作為體外模型,進行了多次超音波刺激。結果顯示,超音波明顯促進了C2C12的融合程度。與未刺激組相比,經超音波刺激後的C2C12在分化第二天時的MyHC3表現量有增加的趨勢,這表明超音波刺激可能增強了C2C12的分化能力。 為了研究壓電對C2C12的影響,我們給與石英玻片超音波以產生電場。培養有C2C12的玻片放置於石英玻片上以進行壓電刺激。結果顯示,壓電導致C2C12細胞核的形態變化,細胞核變小且細長,同時初級纖毛的組裝也得到提升,特別是在刺激後12小時更明顯。我們推測壓電可能通過在早期分化階段促進初級纖毛的組裝,加速C2C12的分化。 在動物實驗中,我們切斷右腿脛神經來建立小鼠肌肉萎縮模型。自第二週開始,我們對小鼠進行了為期三週,每週三次、每次五分鐘的聚焦超音波和壓電治療。研究結果顯示,聚焦超音波和壓電治療明顯抑制了小鼠肌纖維橫截面積的下降,尤其聚焦超音波治療進一步顯著抑制了腓腸肌的重量下降。本研究證實超音波治療改善肌肉萎縮的可能性,並顯示超音波在治療肌肉退化方面具有潛力。 Low-intensity ultrasound has been proven to enhance the proliferation and differentiation of C2C12 cells. The piezoelectric effect has also been demonstrated to reorganize chondrocytes through primary cilia, which play a crucial role in skeletal muscle differentiation. However, the application of ultrasound and piezoelectric stimulation for treating muscle atrophy remains uncertain. Therefore, this study aims to investigate the feasibility of utilizing ultrasound and piezoelectric stimulation for the treatment of muscle atrophy. We selected C2C12 as an in vitro model and conducted multiple rounds of ultrasound stimulation. The results clearly indicate that ultrasound significantly promotes the fusion of C2C12 cells. Compared to the unstimulated group, the C2C12 cells stimulated with ultrasound showed a noticeable increase in MyHC3 expression on the second day of differentiation, suggesting that ultrasound stimulation might enhance the differentiation of C2C12 cells. To explore the impact of piezoelectric stimulation on C2C12 cells, we applied ultrasound to quartz slides to generate an electric field. Quartz slides containing C2C12 cells were placed on the quartz slides to undergo piezoelectric stimulation. The outcomes revealed that piezoelectric stimulation induced morphological alterations in the nuclei of C2C12 cells, resulting in smaller and elongated nuclei. Furthermore, there was an enhanced assembly of primary cilia, particularly evident at 12 hours after stimulation. It is hypothesized that piezoelectric stimulation may promote the assembly of primary cilia during the early stages of differentiation, thereby accelerating the differentiation process of C2C12 cells. In animal study, we established a mouse model of muscle atrophy by transecting the tibial nerve. Starting from the second week, the mice underwent three weeks of focused ultrasound and piezoelectric treatment, administered three times a week for five minutes each session. The findings demonstrated that focused ultrasound and piezoelectric treatment significantly suppressed the reduction in the cross-section area of mouse muscle fibers, with focused ultrasound treatment particularly demonstrating a remarkable inhibition of weight loss in the gastrocnemius muscle. This study confirms the potential of ultrasound treatment in ameliorating muscle atrophy and highlights the potential of ultrasound as a therapeutic modality for muscle degeneration. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/88417 |
DOI: | 10.6342/NTU202301034 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 醫學工程學研究所 |
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