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標題: | 狹葉赤車使者及大冷水麻地上部化學成分之研究 Chemical constituents from the aerial parts of Elatostema lineolatum and Pilea melastomoides |
作者: | Tsun-Yu Lu 呂尊昱 |
指導教授: | 李水盛(Shoei-Sheng Lee) |
關鍵字: | 狹葉赤車使者,大冷水麻,化學成分, Elatostema lineolatum Wight var. majus Wedd.,Pilea melastomoides (Poir.) Wedd.,chemical constituent, |
出版年 : | 2019 |
學位: | 碩士 |
摘要: | 狹葉赤車使者Elatostema lineolatum Wight var. majus Wedd. (Urticaceae)為蕁麻科樓梯草屬之多年生草本植物,分佈於日本、中國及台灣中低海拔之潮濕山區。經由文獻查詢,目前尚無相關成分之研究。而由於同屬植物之低極性成分已有報告,本研究遂著重於中高極性成分之探討,以窺探其潛在的醫療用途。利用Sephadex LH-20管柱、離心分配層析、矽膠管柱、低壓逆相層析管柱及半製備高壓液相層析管柱進行分離,自本植物地上部乙醇萃取物之乙酸乙酯與正丁醇可溶二部分分別分離並鑑定出6個(1~6)及10個(7~16)化合物。
大冷水麻Pilea melastomoides (Poir.) Wedd. (Urticaceae)為蕁麻科冷水麻屬之多年生草本植物,分佈於印度、中國及台灣中低海拔之闊葉林區。本部分主要研究該植物地上部之中高極性成分,探討其潛在的醫療用途。大冷水麻之乙醇萃取物以極性劃分為2%乙酸可溶與不可溶二部分,2%乙酸可溶部分進一步以乙酸乙酯和水進行分配,再劃分成乙酸乙酯和水可溶二部分,其中水可溶部分利用氨水鹼化後再以三氯甲烷抽提,得三氯甲烷可溶部分。水層以XAD-II管柱層析後,得到甲醇沖提物。經由各種層析,分別自乙酸乙酯可溶部分、甲醇沖提物、酸不可溶部分分離並鑑定出2個(17 and 18)、4個(11, 12, 19 and 20)及1個(21)化合物。 上述成分之結構主要係藉由解析核磁共振譜(氫、碳-13與二維圖譜)並搭配質譜資料而得到。其中化合物2為新化合物。化合物1~16, 19, 20及21首次自同屬植物中分離得到。且Wasabiside (3)具有神經保護的作用;Vicenin-2 (13)可促進胰島素分泌;Maltol glucoside (19)具有抑制發炎之生物活性;Stigmast-4-en-3-one (21)於大鼠試驗中具明顯降低血壓之效果。 Elatostema lineolatum Wight var. majus Wedd. (Urticaceae) is a perennial herbaceous plant distributed in the humid forests from low to medium altitudes in Japan, China and Taiwan. As its chemical constituents of low polarity have been reported and its potential medical use remains explored, its chemical constituents of moderate and higher polarity were investigated in this study. The 95% ethanol extract of the aerial part of this plant was divided into fractions soluble in CHCl3, EtOAc, n-BuOH, and H2O via liquid-liquid partitioning process. EtOAc and n-BuOH soluble fractions were separated by Sephadex LH-20 column, cetrifugul partition chromatography, silica gel column, ODS reverse phase column , semi-preperative RP-18 HPLC. These efforts led to isolation of six compounds (1~6) from the EtOAc-soluble fraction and ten compounds (7~16) from the n-BuOH-soluble fraction. Pilea melastomoides (Poir.) Wedd. (Urticaceae) is a perennial herbaceous plant, distributed in the broad-leaved forests from low to medium altitudes in India, China and Taiwan. As this plant is quite abundant in mountain area of northern Taiwan and its potential medical use remains explored, its chemical constituents were investigated in this study. The 95% ethanol extract of the aerial part of this plant was divided into 2% acetic acid-soluble fraction and residue. Acid-soluble part was extracted by EtOAc to give an EtOAc-soluble fraction. The aqueous fraction was basified by ammonia water and then extracted by CHCl3 to give CHCl3-soluble fraction. The aqueous layer was then passed through XAD-II column to give a MeOH-eluted fraction. By various chromatographic methods, two compounds (17 and 18) were isolated and identified from the EtOAc-soluble fraction, four compounds (11, 12, 19 and 20) from the MeOH-eluted fraction, and one compound (21) from the acid-insoluble residue. The strutures of these isolated compounds were elucidated mainly by spectroscopic analysis of 1D and 2D NMR assisted by MS data. Among them compound 2 is new, and compound 1~16, were first found in the Elatostema plants; compound 19, 20 and 21 were first found in the Pilea plants. Amomg these compounds, compound 3 can protect neurons, compound 13 can increase insulin level; compound 19 has anti-inflammatory activity; compound 21 can decrease blood pressure in rats. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/78724 |
DOI: | 10.6342/NTU201902101 |
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顯示於系所單位: | 藥學系 |
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