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Title: | 製備並評估RGD胜肽接枝幾丁聚醣膠作為口服健康食品形成物理阻隔抗肥胖症之功效 The Preparation and Evaluation of a RGD-Conjugated Chitosan Gel as an Orally Administrated Health Food Supplements forming Physical Barrier for Anti-Obesity |
Authors: | Yu-Ting Chen 陳郁婷 |
Advisor: | 林?輝(Feng-Huei Lin) |
Keyword: | 肥胖,健康食品,生醫材料,RGD胜?,幾丁聚醣,交聯, Obesity,Health food,Biomaterials,Chitosan,RGD peptide,Crosslink, |
Publication Year : | 2018 |
Degree: | 碩士 |
Abstract: | 肥胖和過重為異常或過多的脂肪積累,伴隨賀爾蒙失衡、神經傳導激素分泌缺乏,連帶影響身體其他功能的疾病。目前調節體重的方法以健康食品為多數人的最佳選擇,但市面上的健康食品仍有服用順從度低、單方功效等相當程度上的缺點。本研究旨在於研發並評估RGD胜肽接枝幾丁聚醣,仿造醫療器材Endobarrier®套筒形成物理性阻隔減少油脂攝取模式,透過RGD與腸胃上皮細胞的整合素受器達到中長期貼附,並評估減肥功效。
使用BDDE開環接枝RGD胜肽於幾丁聚醣,由比較接枝前後的FTIR官能基圖譜和NMR一維氫譜確認接枝成功。RGD功能性測試顯示短胜肽可抵擋消化液而保有原特徵峰;寧海德林測試量化接枝上的RGD濃度,結果顯示接枝上的RGD胜肽具其功效和接枝效率。 RGD胜肽接枝幾丁聚醣依據ISO 10993進行WST-1測試細胞活性;並使用LIVE & DEAD 測試說明實驗材料具良好生物相容性。並以WST-1篩選材料最佳濃度進行細胞貼附試驗,顯示修飾後的幾丁聚醣較有良好的貼附性。動物實驗以飼料誘發肥胖模型,RGD接枝幾丁聚醣確實能達到體重控制之效,並受劑量高低影響。比較接枝前後幾丁聚醣的體重控制成效,推論經過修飾後的幾丁聚醣較未修飾幾丁聚醣具有控制體重之功效。由體重變化與標準體重增加率得到幾丁聚醣在四周內的體重增加率約為62%、四周後為70%;高低劑量的RGD胜肽接枝幾丁聚醣內臟體脂率相較控制組達顯著差異,但高低劑量間無差異。血清、血液和尿液結果則顯示數值正常。綜合上述結果,RGD胜肽接枝幾丁聚醣膠為一安全性高,適用於短效、降低內臟脂肪以達控制體重的健康食品。未來仍可改量此幾丁聚醣膠,期待研發出安全、長效並兼具全方位效果的抗減肥健康食品。 Obesity and overweight has become a serve issue all over the world. It has been defined as a disease with excessive fat accumulation causing hormone imbalances. Health food is the optimal one; however, it remains low compliance. The aim of this study is to develop and evaluate a RGD-conjugated chitosan gel as an orally administrated health food supplements for anti-obesity treatments. The gel is designed to be like Endobarrier®, as a biocompatible, low risk, middle-term lasting covering the surface of gastrointestinal tract to form an absorption barrier. The FTIR and NMR spectrum indicated that RGD has been conjugated successfully on chitosan; Ninhydrin and RGD functionality test showed large amount of RGD peptide conjugated and effective. In vitro data suggested good biocompatibility. In vivo animal studies showed the gel can lower the weight and reduce the body fat rate affected by dosage. Comparing the weight control effects of conjugated and nonconjugated chitosan, it was deduced that the modified chitosan was effective in controlling weight comparing with the original. Taken together, the RGD conjugated chitosan gel is a safe, suitable for short-term, reducing visceral fat rate health food to control weight. In the future, it is expected to develop a safe and long-term effective anti-obesity chitosan by further modification. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/77644 |
DOI: | 10.6342/NTU201801099 |
Fulltext Rights: | 未授權 |
Appears in Collections: | 醫學工程學研究所 |
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