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標題: | 探討BLMP-1在線蟲腸道細胞內之膜囊泡運輸過程中扮演的角色 Investigating the role of C. elegans BLMP-1 in vesicle trafficking in the intestinal cell |
作者: | Katherine Chen 陳可葳 |
指導教授: | 吳益群 |
關鍵字: | 膜囊泡運輸,blmp-1,rab-11,回收囊泡機制,P-glycoprotein, vesicle trafficking,blmp-1,rab-11,recycling endosomes,P-glycoprotein, |
出版年 : | 2019 |
學位: | 碩士 |
摘要: | 囊泡運輸作用對於生物體來說是極為重要又複雜的機制。細胞透過胞吞作用、胞吐作用,以及物質運輸,來維持膜上物質的動態平衡,包括膜上蛋白質的量及位置。然而,細胞內物質運輸機制還有許多細節尚未被釐清。BLMP-1(B細胞成熟因子)是一個轉錄因子,在演化上高度被保留下來,代表其對於生物體具有重要性。先前研究指出,BLMP-1在生物體的發育過程中,決定特定時間的發育事件,這包含了小鼠免疫細胞的分化作用、果蠅蛻皮激素的分泌以及線蟲遠頂細胞的移動。我們的研究發現blmp-1(s71)線蟲突變株其表皮的鈣黏蛋白(E-cadherin)有錯位的情形。我們猜測這可能源自細胞內囊泡物質運輸情形出現了問題。透過觀察各種細胞內物質運輸相關的蛋白標定線蟲,確實發現到在blmp-1(RNAi)突變種腸道細胞囊泡運輸作用有變異,包含負責將物質運輸到細胞膜表面的RAB-11-related回收囊泡數量減少,以及跟降解作用的囊泡數量異常累積,也可能造成分泌到細胞膜上的P-糖蛋白(P-glycoprotein)的量可能有減少,指向BLMP-1可能透過影響囊泡運輸作用,而調控運送物質到細胞膜上的機制。 Membrane trafficking is an important and complicated mechanism in all cells. The counterbalancing action of endocytosis and exocytosis maintains a dynamic equilibrium that regulates the composition of the plasma membrane as well as the level and localization of membrane proteins. E-cadherins which are regulated by membrane trafficking are adherens junctional molecules important for tissue organization and integrity. BLMP-1 is an evolutionarily conserved transcriptional factor which mostly functions as a repressor. Previous studies have shown that BLMP-1 regulates the timing of specific developmental events including ecdysone induced developmental pathway in Drosophila and distal tip cells migration in C. elegans. In our studies, we used investigated blmp-1 function in C. elegans. Interestingly, we observed that E-cadherin proteins are mislocalized in the epidermis of the blmp-1 mutant. We hypothesized the E-cadherin abnormality might be due to defects in vesicle trafficking. We employed RAB-related reporters to dissect vesicle trafficking steps. The data showed that inactivation of blmp-1 by RNA interference caused the decrease of rab-11-related recycling vesicles, theaccumulation of lysosome-related organelles and the increase of late endosomal vesicles as well as lysosomes. Further, we found that the P-glycoprotein, which localizes to the apical surface of intestinal cells, were decreased in blmp-1(RNAi) mutants. This result indicated that the defects of vesicle trafficking in blmp-1(RNAi) mutants affected the localization of membrane proteins. Altogether, our results suggested that BLMP-1 affects vesicles trafficking in the intestine. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/73571 |
DOI: | 10.6342/NTU201904035 |
全文授權: | 有償授權 |
顯示於系所單位: | 分子與細胞生物學研究所 |
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