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標題: | 乙型微球蛋白與藍瑞斯母豬繁殖能力之關聯性 The Relationship between Beta 2-Microglobulin (B2m) and the Reproductive Performance of the Landrace Sow (Sus scrofa) |
作者: | Ji-Ren Yang 楊己任 |
指導教授: | 莊榮輝 |
關鍵字: | 藍瑞斯母豬,著床,B2-微球蛋白 (B2m),繁殖能力,二維電泳,蛋白質轉印分析, Landrace sow,Implantation,Beta 2-microglobulin (B2m),Reproductive performance,Two-dimensional electrophoresis,Western blot analysis, |
出版年 : | 2012 |
學位: | 碩士 |
摘要: | 雌性哺乳類動物在懷孕時,自身的免疫系統必須要容忍帶有外來抗原的胚胎在體內發育,因此在著床時期正確的調節抗原呈現,母體和胚胎的接觸介面是決定胚胎能否存活的關鍵。MHC (major histocompatibility complex) 是負責在細胞膜上呈現抗原的分子,其中的 B2m (beta 2-microglobulin) 蛋白質在抗原呈現中為一不可或缺的角色。本論文利用 B2m 的單株抗體來觀測 B2m 與母豬繁殖能力之關聯。我們以二維蛋白質轉印法分析了高產母豬和低產母豬於配種前之血液樣本,發現母豬血液中的B2m在分子量28 kDa的位置有8個不同pI的位點,反映了轉譯後修飾的可能;而具有高產能力的母豬血液中的B2m在較低pI值的兩點表現量較低產母豬明顯,因此我們認為高低產母豬可能在 B2m較低pI值的兩點表現量上有所差異。藉由此兩點和pI值較高的幾點之表現量差異,進行生產預測試驗,預測未配種母豬之生產情形 (僅判斷為高產或低產兩個群體)。在進行了四批樣本取自不同月份的試驗後,發現我們較能準確預測涼季配種的母豬群體,兩次的預測準確率為100%;而在溫季和熱季配種的母豬,預測準確率分別為90%和50%,整體的預測準確率為87.8%。因此我們認為母豬在配種時或懷孕時所處之季節溫度可能會影響母豬的生理狀況,進而影響B2m產生預測的錯誤。而B2m為什麼會有不同pI值的位點分佈,季節是否真的會影響我們的預測結果,這些都是未來還需要進一步瞭解的部份。 During pregnancy, the immune system of female mammals tolerates a non-self antigen-presenting embryo. Therefore, a fine-tuned mechanism of antigen presenting between the maternal-fetal interface during implantation is crucial for embryo survival. Antigen presenting requires MHC (major histocompatibility complex) molecules on the cell surface, and one of the components beta 2-microglobulin, is prerequisite for the complex to be located on cell surface. In this study, monoclonal antibody against B2m was used to evaluate the reproductive performance of Landrace sows and its relationship between serum B2m. In two-dimensional Western blot analyses, serum B2m from both high-productive and low-productive premating sows showed several spots around 28 kDa with different pI, which may result from posttranslational modification. Comparing the protein levels between these spots, we found that high-productive sows showed higher protein intensity for the two acidic B2m spots, but not in low-productive sows. Based on this pattern, prediction of productivity performance experiments were conducted to predict the productivity of premating landrace sows. Four batches of serum samples were collected in different mating seasons. The first two predictions of accuracy in cool season were both 100%, and the one in warm season was 90%. However, the one in hot season only reached predicted 50% accuracy. Thus, the seasonal temperature changes may influence the physiological state of pregnant sows, and further affecting the prediction accuracy by serum B2m pattern. In conclusion, the modification of B2m leading to different spots and the seasonal effects need further study to elucidate the relationship between B2m and reproductive performance of sows. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64040 |
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顯示於系所單位: | 生化科技學系 |
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