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標題: | 振動式自感測微懸臂梁應用於凝血反應之監測 A Study on Monitoring Coagulation Reaction by Use of Self-Sensing Microcantilever with External Vibration |
作者: | Yu-Chen Chang 張育禎 |
指導教授: | 黃榮山(Long-Sun Huang) |
關鍵字: | 壓阻,微懸臂梁,凝血?原時間,振動,黏度, piezoresistive microcantilever,prothrombin time,oscillation,viscosity, |
出版年 : | 2012 |
學位: | 碩士 |
摘要: | 在現今社會中,心血管疾病患者越來越多,為了防止血管栓塞,病患常需服用抗凝血劑。為了避免用藥不當,造成出血不止或無法達到療效,常需往返於住家與醫院之間,監測用藥情況;同時,因為在醫療院所的生醫檢驗多半集中操作,完成一個檢驗尚須包含檢體運送、上機排程與報告傳送的時間,因此病患往往需花漫長的時間等待檢驗結果。若希望能節省在住家與醫院間來回以及等待報告的時間與心力,即有賴定點照護—在病患附近進行即時監測—的發展。
本研究成功利用微奈米機電技術開發出具定點照護功能的振動式壓阻式微懸臂梁凝血感測器,可用於監測凝血酶原時間—口服抗凝劑用藥監測的評估方式。本研究參考測量血液凝固情況的Sonoclot分析儀,利用外部振動器振動微懸臂梁,使其可在待測樣品中振動以感測樣品的黏度變化。本研究以可屏除外界雜訊與不必擔心微懸臂梁因黏度太大而斷裂為考量,選擇10 Hz的振動頻率為實驗所用的參數。在對不同黏度的液體感受性實驗中,使用不同濃度之甘油水溶液進行實驗,結果可得微懸臂梁內壓阻阻值在每一次振動下改變量之振幅和黏度的關係,其斜率為0.00869 Ω/cP, ,線性度相當高,確定振動式壓阻式微懸臂梁確實能分辨不同黏度之液體。將懸臂梁用於測量實際凝血情況,其對於凝血酶原時間的量測具有相當高的準確性:對於濃度1、2、3之血凝品管液,測得知凝血酶原時間範圍分別為10.7~12.4秒、22.6~26.9秒、32.3~37.7秒,結果幾乎都可落在品管液提供的參考值之內;量測結果具有特定的圖形,可反應凝血時纖維蛋白的生成與纖維蛋白聚集形成血塊的階段;同時,結合不同黏度液體中的量測結果,可了解血凝過程黏度的變化,本研究中濃度1、2、3之血凝品管液在纖維蛋白凝塊形成時,黏度最高分別可達504.3 cP、216.6 cP、104.5 cP。可監測血凝過程黏度變化是本感測器的一大特點。 本研究所開發之振動式壓阻式微懸臂梁凝血感測器,若技術成熟時,可大量生產降低成本;除可應用於量測凝血酶原時間外,在其他血凝相關檢測上也有相當的潛力;與Sonoclot分析儀相比,除能維持其詳細描繪凝血過程的特性,且具微小化、可測量凝血酶原時間、了解凝血過程黏度變化情況等優點。綜合以上結論,此感測器未來將具有相當大的發展潛力。 This work has successfully developed a miniaturized, real-time coagulation monitoring sensor by using an externally vibrated, self-sensing piezoresistive microcantilever for disposable point-of-care coagulation. With the increased use of oral anti-coagulant drugs and increasing adverse drug events, the need for point-of-care coagulation devices has become necessary for timely intervention of anticoagulant therapy. The prothrombin time (PT) is a measure of the extrinsic pathway of blood coagulation to determine the clotting development of blood. The measurement employs the Sonoclot measurement method in which an oscillated probe is immersed in a sample probe and the signal is measured by the changes in impedance to movement imposed by the developing clot. In this study, the measurement was performed by moving the piezoresistive cantilever immersed in a sample liquid at a fixed frequency of 10 Hz with an external vibrator, and recording the amplitude deflection or associated changes in resistance of a cantilever for sensing dynamic viscosities of clot formation. The resistance changes of a microcantilever exhibited an excellent linear correlation in calibration with viscosity changes of glycerol/water solutions. The linear correlation was obtained in a slope with 0.00869 Ω/cP. Likewise, three types of commercially standard samples for coagulation prothrombin time measurements were used to test the coagulation of microcantilever sensor. The measured results of resistance changes with specific patterns of signature indicated the viscoelastic changes of commercially standard sample coagulation. The onset of upward dramatic change in amplitude deflections or resistances was defined as the prothrombin time (PT). This microcantilever sensor is promising in prothrombin time measurement for point-of-care coagulation monitoring. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/56718 |
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顯示於系所單位: | 應用力學研究所 |
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