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標題: | FIP-fve 蛋白之 N 端序列對其免疫活性之必要性 N-terminal of FIP-fve is essential for its immune activity |
作者: | Yu-Shan Lin 林瑜珊 |
指導教授: | 許 輔(Fuu Sheu) |
關鍵字: | 超抗原,His 融合蛋白,同型雙元體,免疫調節蛋白,共同免疫沉澱, superantigen,His-fusion protein,homodimer,immunomodulatory protein,co-immunoprecipitation, |
出版年 : | 2014 |
學位: | 碩士 |
摘要: | 金針菇免疫調節蛋白 FIP-fve 萃取自金針菇子實體,文獻指出其具有免疫調節、抗癌、抗過敏及減緩發炎反應等功效。二級結構分析顯示,FIP-fve 透過兩個單元體 N 端 α-helix 以疏水性鍵結形成雙元體。本實驗室前人研究指出 FIP-fve 不需經過抗原呈獻細胞修飾,而是透過與 MHC 及 TCR 分子鍵結活化 T 細胞,因此推測FIP-fve 作用方式類似超抗原。本研究目的有二,一為證實雙元體結構對於 FIP-fve 的免疫調節活性是必要的,二為找出 FIP-fve 與 MHC 和 TCR 具交互作用之證據。為探討雙元體結構對 FIP-fve 免疫調節活性之影響,將 1-13 胺基酸缺失或全長 FIP-fve cDNA構築於 pET-32a(+) 載體,並透過大腸桿菌系統表現得分子量皆約 26 kDa 之 His-FIP-fve 1-114 和 His-FIP-fve 14-114 蛋白。以 enterokinase 切除 His-tag 後,經質譜比對確認成功取得 rFIP-fve 1-114 以及 rFIP-fve 14-114。免疫調節活性試驗方面,片段缺失 rFIP-fve 14-114 刺激小鼠脾臟細胞分泌 IFN-γ 能力較 rFIP-fve 1-114 弱,且無法刺激小鼠脾臟細胞 IFN-γ 及 IL-2 基因表現。綜合以上結果,推測失去雙元體結構造成 FIP-fve 免疫調節活性降低。在 FIP-fve 類超抗原性質探討上,透過共同免疫沉澱法可知 FIP-fve 與表面分子 MHC class II 具交互作用,另外發現 FIP-fve 可被 MHC class I 及其 Isotype IgG2a 抗體捕捉。然而,本研究尚未透過共同免疫沉澱法看見 FIP-fve 與 TCR 連結之證據,因此僅能證實 FIP-fve 能連結 APCs 表面分子 MHC class II。 FIP-fve, an immunomodulatory protein isolated from Flammulina velutipes, has been demonstrated to have activities of immunomodulatory, anti-cancer, anti-allergic and suppression of inflammation. Based on a secondary structure analysis, we known that two FIP-fve monomers form homodimer through the hydrophobic interaction between N-terminal α-helics. Previous studies suggested that FIP-fve can mediate T cells activation through the linkage between T cell receptors (TCR) on T cells and MHC molecules on antigen present cells (APCs) without processing procedure of APCs. This process is similar to the mechanism of superantigen-mediated immune response. The aim of this study was to demonstrate the necessity of dimer structure for FIP-fve immunomodulatory activity and to prove the directly binding of FIP-fve with MHC and TCR. To study the relationship between the dimer structure and immunomodulatory activity of FIP-fve, we constructed the full length 1-114 FIP-fve cDNA and truncated 14-114 FIP-fve cDNA on pET-32a(+) vector and expressed the His-fusion protein His-FIP-fve 1-114 and His-FIP-fve 14-114. After digested by enterokinase to remove the His-tag residue, rFIP-fve 1-114 and rFIP-fve 14-114 were purified with FPLC system. The ability of rFIP-fve 14-114 to stimulate IFN-γ production on murine splenocytes was lower than rFIP-fve 1-114. Incubating murine splenocytes with rFIP-fve 14-114 could not elevate the gene expression of IFN-γ and IL-2. According to the above results, we suggested that the dimerization might be critical for FIP-fve immunomodulatory activity. On the other hand, FIP-fve and RAW 264.7 cell lysate were co-immunoprecipitated to determine superatigen-like characterization of FIP-fve. Results indicated that FIP-fve could interact with MHC class II molecule directly. Interestingly, we also found that FIP-fve was pulled down by both anti-MHC class I antibody and IgG2a which was isotype of anti-MHC class I antibody. However, no co-immunoprecipitated evidence showed that FIP-fve bound with TCR in this study. In conclusion, we proved that FIP-fve directly bind with MHC class II molecule. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/5225 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 園藝暨景觀學系 |
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