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標題: | 建立自然殺手細胞淋巴癌及霍奇金病細胞株的microRNA資料庫 Construction of microRNA libraries in NK-cell and Hodgkin lymphoma cell lines |
作者: | Mi-Hsin Huang 黃宓昕 |
指導教授: | 林中梧 |
關鍵字: | 淋巴癌,霍奇金病,資料庫, lymphoma,Hodgkin lymphoma,microRNA,library, |
出版年 : | 2008 |
學位: | 碩士 |
摘要: | MicroRNA (miRNA) 是一種有調節能力的小分子的RNA,作為基因轉錄後的調控者。由於在基因表現上扮演重要角色,研究基因體 (genome)中miRNA的表現,傳統方法常使用定量反轉錄聚合酶鏈式反應(qRT-PCR) 或微陣列 (array) 分析miRNA。在這篇論文中,我們利用一種新的技術來分析基因體中miRNA的表現。藉由高效液相色層分析法(HPLC)純化出不同細胞株之miRNA,分別定序每一個選殖株 (clone),並藉由生物資訊的方法來比對。每一個資料庫中約30-40%的選殖株為已知可信的miRNA MicroRNAs (miRNAs) are a class of small regulatory RNAs, which act as post-transcriptional modulators of gene expression. Because of their critical roles in gene expression, genome-wide profiling of miRNAs, by qRT-PCR or array, has become an essential step in understanding cellular biology. In this thesis, we have developed a novel method for constructing miRNA libraries, as an initial step toward genome-wide miRNA characterization. We purified and cloned miRNAs by HPLC from several cell lines. Individual clones were sequenced, and identified by bioinformatics. It was estimated that about 30-40% of the clones from each library represented authentic miRNAs. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26714 |
全文授權: | 未授權 |
顯示於系所單位: | 病理學科所 |
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