壬基苯酚降解性海洋細菌分離株之特性研究

Abstract

壬基苯酚(nonylphenol)為非離子型界面活性劑，廣泛運用在工業和家庭用途，會隨廢水排放到環境中。此化合物具有毒性，結構和動物體內的雌性激素相似，在生物體內會干擾正常內分泌系統運作，因而被視為環境荷爾蒙。本實驗的目的在於先從台灣沿岸海水，分離出具壬基苯酚降解能力的菌株，進而探討其特性。根據 16S rDNA 親緣分析得知具較強壬基苯酚降解活性的35 株分離株，可歸類 於氣單胞菌屬(Aeromonas )、弧菌屬(Vibrio)、交替單胞菌屬（Alteromonas）、沙雷菌屬(Shewanella )和擬交替單胞菌屬(Pseudoalteromonas)等屬。針對其中10株壬基苯酚降解能力較強的分離株，利用 HPLC 分析其培養七天的菌液，發現壬基苯酚剩降解為 20%~30%，由此看來，菌液中減少的壬基苯酚之苯環，應多已被 降解。

Nonylphenol (NP) is a nonionic surfactant used in many industrial and domestic applications, belonging to a group of pollutants which are widely distributed in aquatic environment. NP is toxic and possessing the ability to disrupt the function of endocrine system by mimicking hormone, blocking or stimulating hormonal production. The objective of this study was to isolate and characterize the bacterial strains capable of degrading NP from coastal seawaters of Taiwan The phylogenetic analysis according to 16S rDNA sequences revealed that 35 strong degrading activity isolates belonged to genus Vibrio, Aeromonas, Alteromonas, Shewanella and Pseudoalteromonas, respectively. Through the test of NP removal, 10 isolates exhibited the highest rate of degradation of NP, when compared with the other isolates. The results showed that NP was readily degraded by theses isolates with 20-30% of the total NP being removed within 7 days. It is apparent that NP should have already been degraded by these isolates