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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳建甫 | zh_TW |
| dc.contributor.advisor | Chien-Fu Chen | en |
| dc.contributor.author | 王琳淯 | zh_TW |
| dc.contributor.author | Lin-Yu Wang | en |
| dc.date.accessioned | 2025-09-17T16:14:04Z | - |
| dc.date.available | 2025-09-18 | - |
| dc.date.copyright | 2025-09-17 | - |
| dc.date.issued | 2025 | - |
| dc.date.submitted | 2025-08-11 | - |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/99640 | - |
| dc.description.abstract | 在傳統藥物篩選流程中，人工分注細胞的過程不僅耗時，同時也限制實驗通量的提升。為此，本研究開發了一應用於高通量藥物篩選的微流體細胞分注裝置，可實現快速且自動化地將細胞分注至實驗孔盤中。本裝置設計基於微流道中流體所呈現的層流特性，以 Hagen-Poiseuille 方程式進行幾何設計以達下游流道等效流阻，使細胞懸浮液於注射幫浦所產生之壓力驅動下，透過流道結構引導至各出口。裝置同時引入被動式擾動器，提升出口細胞的均一性。在裝置性能的驗證中，研究使用 10 μm 之聚苯乙烯微粒作為細胞的模擬模型，初步確認裝置均勻分流性能。後續使用人類結腸腫瘤癌細胞株 (HCT-116) 進行細胞的分流測試，並透過 AlamarBlue 試劑進行細胞活性的測試。最後以化療藥物 5-FU (5-Fluorouracil) 對 HCT-116 進行細胞毒性的測試，以驗證本裝置於高通量藥物篩選流程中的應用可行性。實驗結果顯示，此微流體裝置可於 2 分鐘內完成 96 孔盤的分注，且輸出之總粒子數量與目標值誤差控制於 7.933% 以內。後續細胞分流實驗亦顯示輸出至孔盤之細胞數量具有高再現性，且細胞經裝置分注後可正常貼附，未見變形與污染現象，顯示裝置具備良好的操作安全性與生物相容性。最終，細胞毒性測試展示經裝置分注之細胞可保有對藥物刺激的反應能力，證實裝置適用於高通量藥物篩選場景。本研究開發之微流體裝置可在短時間內完成細胞分注，預期其可在藥物篩選領域為中小型研究單位與臨床場域提供一低成本、高效率的自動化平臺。 | zh_TW |
| dc.description.abstract | Manual cell dispensing in conventional drug screening workflows is not only labor-intensive, but also limits overall experimental throughput. To address this bottleneck, this study develops a microfluidic cell dispensing device for high-throughput drug screening, enabling rapid and automated cell dispensing into well plates. To ensure uniform flow distribution across outlets, the device leverages fluid's laminar flow characteristics within microchannels, where downstream channel geometries are designed according to the Hagen-Poiseuille equation to achieve equivalent hydraulic resistance. Driven by a single syringe pump, cell suspension is guided through the channel network to multiple outlets. Furthermore, integrated passive perturbation structures enhance the uniformity of the dispensed cells at the outlets. The device's performance is initially validated using 10 µm polystyrene beads as a cell mimic, confirming its capability for uniform distribution. Subsequently, human colon carcinoma cell line (HCT-116) is used to evaluate cell aliquoting performance, with cell viability assessed via the AlamarBlue assay. Ultimately, chemotherapeutic drug 5-Fluorouracil (5-FU) is used to perform a cytotoxicity test on the dispensed HCT-116 cells, validating the device's applicability in a high-throughput drug screening workflow. Results demonstrate that the microfluidic device can complete dispensing a 96 well plate within 2 minutes, with the deviation of the total particle count at each outlet controlled within 7.933% of the target value. The device exhibits good biocompatibility, operational consistency, and high reproducibility in dispensing viable cells, as cells adhere properly with no observable deformation or contamination. Lastly, cytotoxicity test shows cells dispensed by the device retained their ability to respond to drug stimuli. This work presents an efficient solution for cell dispensing in high-throughput drug screening, expects to offer a cost-effective platform for biomedical research and clinical settings in drug screening. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2025-09-17T16:14:04Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2025-09-17T16:14:04Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 致謝 i
摘要 ii Abstract iii 目次 v 圖次 viii 第一章、前言與文獻回顧 1 1.1 癌症治療的現況與挑戰 1 1.2 藥物探索 1 1.3 高通量藥物篩選與其挑戰 2 1.4 微流體之細胞操控技術 2 1.5 外部物理場域 3 1.5.1 介電泳力控制 3 1.5.2 聲學控制 4 1.5.3 磁學控制 5 1.6 流體動力學 6 1.6.1 閥門控制 6 1.6.2 結構限制方法 7 1.6.3 垂直重力方法 8 1.7 本研究開發之裝置 9 第二章、實驗設計與實驗流程 11 2.1 實驗試劑與耗材 11 2.2 實驗儀器 12 2.3 微流體裝置製作 13 2.3.1 微流體裝置分流流道設計 13 2.3.2 微流體裝置製作 14 2.4 實驗溶液配置 15 2.4.1 粒子懸浮液配置 15 2.4.2 不同黏度溶液配置 16 2.4.3 結腸腫瘤細胞培養與細胞懸浮液配置 16 2.4.4 5-FU 藥物溶液配置 16 2.5 實驗步驟 16 2.5.1 分流流道數值模擬分析 16 2.5.2 被動式擾動器引入 17 2.5.3 不同注入流率之出口體積量與粒子數量均一性測試 17 2.5.4 不同黏度溶液分流測試 18 2.5.5 不同濃度之粒子懸浮液分流測試 18 2.5.6 微流體裝置出口擴充性性能驗證 18 2.5.7 結腸腫瘤細胞於裝置分流測試 18 2.5.8 結腸腫瘤細胞毒性測試 19 第三章、實驗結果與討論 20 3.1 微流體裝置流道設計 22 3.1.1 分流流道數值模擬分析 22 3.1.2 被動式擾動器引入 23 3.2 聚苯乙烯微粒於微流體裝置功能驗證 25 3.2.1 不同注入流率之出口體積量與粒子數量均一性測試 25 3.2.2 不同黏度與粒子濃度溶液測試 27 3.3 微流體裝置出口擴充性性能驗證 28 3.4 結腸腫瘤細胞分流與毒性測試 30 3.4.1 結腸腫瘤細胞於裝置分注測試 30 3.4.2 結腸腫瘤細胞毒性測試 31 第四章、結論與未來展望 33 參考文獻 34 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 微流體裝置 | zh_TW |
| dc.subject | 高通量 | zh_TW |
| dc.subject | 藥物篩選 | zh_TW |
| dc.subject | 細胞分注 | zh_TW |
| dc.subject | 被動式擾動器 | zh_TW |
| dc.subject | cell dispensing | en |
| dc.subject | microfluidic device | en |
| dc.subject | high-throughput | en |
| dc.subject | passive perturbator | en |
| dc.subject | drug screening | en |
| dc.title | 微流體細胞分注裝置應用於高通量藥物篩選之研發 | zh_TW |
| dc.title | Development of Microfluidic Cell Dispensing Device for High-throughput Drug Screening Application | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 113-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 周逸儒;余政儒;林佳慧 | zh_TW |
| dc.contributor.oralexamcommittee | Yi-Ju Chou;Cheng-Ju Yu;Jia-Hui Lin | en |
| dc.subject.keyword | 微流體裝置,高通量,藥物篩選,細胞分注,被動式擾動器, | zh_TW |
| dc.subject.keyword | microfluidic device,high-throughput,drug screening,cell dispensing,passive perturbator, | en |
| dc.relation.page | 39 | - |
| dc.identifier.doi | 10.6342/NTU202503783 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2025-08-13 | - |
| dc.contributor.author-college | 工學院 | - |
| dc.contributor.author-dept | 應用力學研究所 | - |
| dc.date.embargo-lift | N/A | - |
| 顯示於系所單位： | 應用力學研究所 | |
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