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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 何銘洋 | zh_TW |
| dc.contributor.advisor | Ming-Yang Ho | en |
| dc.contributor.author | 洪子恬 | zh_TW |
| dc.contributor.author | Tzu-Tien Hung | en |
| dc.date.accessioned | 2025-09-10T16:11:21Z | - |
| dc.date.available | 2025-09-11 | - |
| dc.date.copyright | 2025-09-10 | - |
| dc.date.issued | 2025 | - |
| dc.date.submitted | 2025-07-24 | - |
| dc.identifier.citation | Bailey, Timothy L, Johnson, James, Grant, Charles E, & Noble, William S. (2015). The MEME suite. Nucleic acids research, 43(W1), W39–W49.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/99405 | - |
| dc.description.abstract | 利用藍綠菌作為平台的合成生物學近年來蓬勃發展。透過於藍綠菌中誘導表現特定基因，可調控生產生技產品的時機和產量。然而，化學誘導成本高且現有的光誘導啟動子僅適用於少數藍綠菌菌株。本研究旨在開發可廣泛應用於藍綠菌的光誘導表達系統。本研究以Chlorogloeopsis fritschii PCC 9212 作為平台，探討兩種光調控啟動子系統。首先，我們針對遠紅光誘導啟動子PchlFJSC1，設計不同長度的截短版本，並以黃色螢光蛋白作為報導基因評估其在遠紅光下的誘導能力。結果顯示完整啟動子表現活性最佳，截短版本雖仍具活性但誘導效果下降，顯示上游序列能增強啟動子活性。其次，我們分析不同色光對C. fritschii PCC 9212光合色素及相關基因表現的影響，藉此尋找可被藍光誘導的基因。結果發現藍光能顯著提升藻膽蛋白含量，並影響藻膽蛋白體及光系統二的相關基因表現。綜上所述，本研究不僅進一步分析了遠紅光誘導的啟動子系統，也為開發藍光誘導的啟動子提供了基礎，有助於未來藍綠菌光誘導表達系統的建立。 | zh_TW |
| dc.description.abstract | Synthetic biology in cyanobacteria has rapidly advanced. By inducing the expression of specific genes, it is possible to regulate the timing and yield of bioproducts. However, chemical inducers are costly and not eco-friendly, and current light-inducible promoters work only in limited strains. This study aims to develop applicable light-inducible gene expression systems in cyanobacteria. Using Chlorogloeopsis fritschii PCC 9212 as a model, we explored types of light-inducible promoter systems. First, the far-red light-inducible promoter PchlFJSC1 was truncated into several versions and evaluated for inducibility under far-red light using a reporter gene. The full-length promoter showed the strongest activity, while truncated versions had reduced induction, indicating that upstream sequences enhance promoter activity. Second, we examined the effect of different light qualities on pigment content and gene expression to identify potential blue light-responsive genes. Blue light increased phycobiliprotein levels and altered the expression of genes related to phycobilisomes and Photosystem II. This study not only provides further insight into far-red light-inducible promoters but also lays the foundation for developing blue light-inducible promoters, supporting the development of light-inducible systems in cyanobacteria. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2025-09-10T16:11:21Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2025-09-10T16:11:21Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 誌謝 i
中文摘要 ii ABSTRACT iii CONTENTS iv LIST OF FIGURES vii LIST OF TABLES viii Chapter 1 Introduction 1 1.1 Synthetic biology in cyanobacteria 1 1.1.1 Basic concept in synthetic biology of cyanobacteria 1 1.1.2 Limitation of synthetic biology of cyanobacteria 2 1.1.3 Regulation of gene expression in cyanobacteria 3 1.2 Photoacclimation in cyanobacteria 5 1.2.1 Far-red light Photoacclimation 5 1.2.2 Blue light photoacclimation 7 1.3 Construction of overexpression system in C. fritschii PCC 9212 9 1.3.1 Overexpression system 9 1.3.2 FRL-inducible promoter 10 1.4 Aim of this study 11 Chapter 2 Material and methods 12 2.1 Strains and growth conditions 12 2.2 Growth curve, pigment extraction 13 2.3 Absorbance and fluorescence spectra 15 2.4 RNA extraction and gene expression profiling 15 2.4.1 RNA extraction 15 2.4.2 Reverse transcription PCR 16 2.4.3 Quantitative real-time PCR 17 2.5 Selection of promoters 17 2.6 Cloning methods 18 2.6.1 Design of the plasmids, primers, and expression constructs 18 2.6.2 DNA source 18 2.6.3 Polymerase Chain Reaction (PCR) 18 2.6.4 Plasmid extraction 19 2.6.5 Chemical competent cell and transformation of E. coli 19 2.6.6 Restriction enzyme digestion and DNA fragments ligation 20 2.6.7 Colony PCR and sequencing 21 2.7 Construction of plasmids 21 2.8 Conjugative transformation of cyanobacteria 22 2.9 Promoter assay of cyanobacteria 23 Chapter 3 Results 25 3.1 Blue light did not significantly inhibit the growth of C. fritschii PCC 9212 25 3.2 Blue light enhances phycobilisome absorption and preferential energy transfer to PSII in C. fritschii PCC 9212 26 3.3 Blue light induces an increase in PBS content without altering Chl a or carotenoids levels in C. fritschii PCC 9212 27 3.4 Blue light affected the transcript level of the genes related to PSII and PBSin C. fritschii PCC 9212 28 3.5 Truncation of the FRL-inducible promoter reduces expression strength 30 Chapter 4 Discussion 32 4.1 Distinct photoacclimation of C. fritschii PCC 9212 to blue light 32 4.1.1 Growth rate and PSI: PSII ratio 32 4.1.2 Phycobilisome content and gene expression 33 4.2 Loss of upstream regulatory elements reduces far-red light-induced promoter activity 35 4.3 Unfinished work 37 4.4 Future work 38 Chapter 5 Conclusions 40 Figures 41 Tables 64 Supplementary Data 84 References 91 | - |
| dc.language.iso | en | - |
| dc.subject | 遠紅光馴化 | zh_TW |
| dc.subject | 藍綠菌 | zh_TW |
| dc.subject | 光誘導啟動子 | zh_TW |
| dc.subject | 藍光馴化 | zh_TW |
| dc.subject | 合成生物學 | zh_TW |
| dc.subject | Chlorogloeopsis fritschii PCC 9212 | zh_TW |
| dc.subject | 遠紅光 | zh_TW |
| dc.subject | 藍光 | zh_TW |
| dc.subject | synthetic biology | en |
| dc.subject | Chlorogloeopsis fritschii PCC 9212 | en |
| dc.subject | light-inducible promoter | en |
| dc.subject | cyanobacteria | en |
| dc.subject | blue light photoacclimation | en |
| dc.subject | far-red light photoacclimation (FaRLiP) | en |
| dc.subject | blue light | en |
| dc.subject | far-red light | en |
| dc.title | 探討藍綠菌 Chlorogloeopsis fritschii PCC 9212 之光誘導調控系統 | zh_TW |
| dc.title | Investigating Light-Inducible Systems in Cyanobacterium Chlorogloeopsis fritschii PCC 9212 | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 113-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 周信宏 ;謝旭亮;朱修安 | zh_TW |
| dc.contributor.oralexamcommittee | Hsin-Hung Chou;Hsu-Liang Hsieh;Hsiu-An Chu | en |
| dc.subject.keyword | 遠紅光,藍光,遠紅光馴化,藍光馴化,藍綠菌,光誘導啟動子,Chlorogloeopsis fritschii PCC 9212,合成生物學, | zh_TW |
| dc.subject.keyword | far-red light,blue light,far-red light photoacclimation (FaRLiP),blue light photoacclimation,cyanobacteria,light-inducible promoter,Chlorogloeopsis fritschii PCC 9212,synthetic biology, | en |
| dc.relation.page | 96 | - |
| dc.identifier.doi | 10.6342/NTU202502040 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2025-07-25 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 生命科學系 | - |
| dc.date.embargo-lift | 2030-07-22 | - |
| 顯示於系所單位： | 生命科學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-113-2.pdf 未授權公開取用 | 2.91 MB | Adobe PDF | 檢視/開啟 |
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