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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 呂俊毅 | zh_TW |
| dc.contributor.advisor | Jun-Yi Leu | en |
| dc.contributor.author | 阮氏銀江 | zh_TW |
| dc.contributor.author | Nguyen Thi Ngan Giang | en |
| dc.date.accessioned | 2025-08-05T16:05:57Z | - |
| dc.date.available | 2025-08-06 | - |
| dc.date.copyright | 2025-08-05 | - |
| dc.date.issued | 2025 | - |
| dc.date.submitted | 2025-07-23 | - |
| dc.identifier.citation | Abresch, H., T. Bell and S. R. Miller (2024). "Diurnal transcriptional variation is reduced in a nitrogen-fixing diatom endosymbiont." ISME J 18(1).
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/98369 | - |
| dc.description.abstract | 在內共生過程中，共生體整合進宿主細胞會顯著改變基因表現與細胞身分。雖然選擇性剪接已知可透過快速調節基因表現與蛋白質同功異構體的多樣性來促進細胞適應，但其在內共生期間扮演的調控角色仍所知甚少。Paramecium bursaria 是研究此現象的理想模式生物，因其細胞質中攜帶數百個 Chlorella variabilis 藻類共生體。此外，P. bursaria 及其相關的 Paramecium 物種具有極短的內含子（中位數約為 24 個核苷酸），因此為探討短內含子動態剪接、內共生期間的調控模式，以及纖毛蟲內含子演化提供了寶貴的系統。在本研究中，我們對含共生體（綠色）與無共生體（白色）的 P. bursaria 細胞進行時間進程RNA 定序，以分析內共生期間的內含子剪接模式。一般而言，5’端近端內含子的剪接，有助於提高基因表現量。我們發現綠色與白色的 P. bursaria細胞之間存在差異性剪接的內含子，且在跨膜轉運蛋白基因中有顯著富集，這些基因對於內共生期間宿主與藻類共生體間的養分交換至關重要。此外，我們鑑定出一系列剪接增強子與抑制子，位於保守的剪接體基因中，其表現量與內共生期間的差異性剪接內含子密切相關。透過跨纖毛蟲的內含子直系同源性分析，我們發現保留的內含子具有較高的剪接效率、較低的 GC 含量以及一致的內含子長度，顯示新演化出的內含子會經過剪接與表現上的優化。我們的研究結果提供了選擇性剪接在宿主於內共生過程中適應角色的新見解，並解釋了短內含子在真核基因體中的演化動態。 | zh_TW |
| dc.description.abstract | The integration of symbionts into host cells during endosymbiosis significantly alters gene expression and cell identity. While alternative splicing facilitates cellular adaptation through rapid modulation of gene expression and protein isoform diversity, its regulatory role during endosymbiosis remains poorly understood. Paramecium bursaria represents an ideal model for investigating this, as it harbors hundreds of Chlorella variabilis algae within its cytoplasm. Furthermore, P. bursaria and related Paramecium species possess exceptionally short introns (median ~24 nucleotides), providing a valuable system for examining short intron splicing dynamics, regulatory patterns during endosymbiosis, and ciliate intron evolution. In this study, we conducted time-course RNA sequencing on symbiont-bearing (green) and symbiont-free (white) P. bursaria cells to analyze intron splicing patterns during endosymbiosis. While in general, splicing, especially 5’ proximal introns, enhances gene expression, we identified differentially spliced introns between green and white, with an enrichment in transmembrane transporter genes, which are crucial for establishing nutrient transport between the host cell and its algal symbionts during endosymbiosis. Additionally, we identified splicing enhancers and repressors among conserved spliceosome genes, whose expression was closely linked to differentially spliced introns during endosymbiosis. Through intron orthology analysis across ciliates, we found that conserved introns are spliced more efficiently, with lower GC content and uniform length, suggesting that newly evolved introns undergo refinement to optimize splicing and expression. Our findings provide insight into the role of alternative splicing in host adaptation during endosymbiosis while shedding light on the evolutionary dynamics of short introns in eukaryotic genomes. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2025-08-05T16:05:57Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2025-08-05T16:05:57Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | Acknowledgements i
摘要 iii Abstract v Table of Contents vi Introduction 1 Materials and Methods 6 Paramecium bursaria strain and culture conditions 6 Genome annotation file and intron annotation 7 RNA extraction and RNA sequencing 7 Gene expression analysis 8 Alternative splicing event analysis, intron retention rate (PSI) calculation & differential spliced intron (DSI) clustering 8 GO terms enrichment analysis 9 Spliceosome comparison using reciprocal best BLAST hit and snRNA finding in P. bursaria 10 Linear regression analysis of the relationship between PSI and splicing-related gene expression in P. bursaria 11 Intron orthologs between ciliate species and intron ages assignment 11 Data availability 12 Results 12 Extremely short introns in P. bursaria exhibit enhancement effect on gene expression level 12 Conservation of the U2-dependent spliceosome in P. bursaria 14 Intron retention and alternative 3’ splice site are the most abundant alternative splicing events in P. bursaria 16 Intron retention rates are associated with GC content, intron length and gene expression levels 17 Two distinct intron splicing patterns between symbiotic and aposymbiotic cells and their association with the expression of splicing factors in symbiotic cells 19 Intron evolution in ciliate species reveals higher splicing efficiency and lower intron GC content in aged introns 22 Alternative splicing as a mode for gene regulation in endosymbiosis 25 Splicing factors specifically regulate DSIs between symbiotic and aposymbiotic cells 26 Intron evolution is associated with gene expression regulation 28 References 31 Figures 44 Tables 66 | - |
| dc.language.iso | en | - |
| dc.subject | 內共生 | zh_TW |
| dc.subject | Paramecium | zh_TW |
| dc.subject | 剪接體保守性 | zh_TW |
| dc.subject | 內含子進化 | zh_TW |
| dc.subject | 可變剪接 | zh_TW |
| dc.subject | Paramecium | en |
| dc.subject | endosymbiosis | en |
| dc.subject | spliceosome conservation | en |
| dc.subject | intron evolution | en |
| dc.subject | alternative splicing | en |
| dc.title | Paramecium bursaria 內含子：內共生期間的剪接調控與纖毛蟲物種中短內含子的演化 | zh_TW |
| dc.title | Paramecium bursaria introns: splicing regulation during endosymbiosis and short intron evolution in ciliate species | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 113-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.coadvisor | 林倩伶 | zh_TW |
| dc.contributor.coadvisor | Chien-Ling Lin | en |
| dc.contributor.oralexamcommittee | 張典顯;蔡懷寬;駱乙君 | zh_TW |
| dc.contributor.oralexamcommittee | Tien-Hsien Chang;Huai-Kuang Tsai;Yi-Jyun Luo | en |
| dc.subject.keyword | Paramecium,內共生,可變剪接,內含子進化,剪接體保守性, | zh_TW |
| dc.subject.keyword | Paramecium,endosymbiosis,alternative splicing,intron evolution,spliceosome conservation, | en |
| dc.relation.page | 66 | - |
| dc.identifier.doi | 10.6342/NTU202502289 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2025-07-25 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 基因體與系統生物學學位學程 | - |
| dc.date.embargo-lift | 2025-08-06 | - |
| Appears in Collections: | 基因體與系統生物學學位學程 | |
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| File | Size | Format | |
|---|---|---|---|
| ntu-113-2.pdf | 4.45 MB | Adobe PDF | View/Open |
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