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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 于昌平 | zh_TW |
| dc.contributor.advisor | Chang-Ping Yu | en |
| dc.contributor.author | 袁輔瑩 | zh_TW |
| dc.contributor.author | Fu-Ying Yuan | en |
| dc.date.accessioned | 2025-08-01T16:06:50Z | - |
| dc.date.available | 2025-08-02 | - |
| dc.date.copyright | 2025-08-01 | - |
| dc.date.issued | 2025 | - |
| dc.date.submitted | 2025-07-25 | - |
| dc.identifier.citation | 石丞均。(2022)。評估3D生物列印於微生物固定化之應用:以尿素分解菌與重金屬去除為例。碩士 (國立臺灣大學)。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/98295 | - |
| dc.description.abstract | 氮污染為水體環境中常見且具累積性之污染問題，傳統硝化–反硝化程序需外加碳源與高能耗，而厭氧氨氧化(ANAMMOX)為一種自營型脫氮機制，具有低碳源需求與低污泥產量等優勢，逐漸受到重視。然ANAMMOX菌生長速率緩慢，對操作條件敏感，故穩定富集與固定化應用成為研究重點。本研究以建立穩定之 ANAMMOX 反應系統為目標，並探討固定化材料與保存條件對其活性與應用可行性之影響。
本研究以一垃圾掩埋場滲出水處理廠生物處理單元活性污泥與食品廠廢水處理廠之活性污泥為接種源，建立實驗室規模之ANAMMOX反應系統，並採漸升氮源濃度操作模式，最終進流總氮濃度為120 mg-N/ L。第94天起觀察到氨氮與亞硝酸氮穩定消耗，顯示系統成功富集ANAMMOX菌群，後續以此建立四組反應槽進行馴養。反應系統最終去氮效率達 60 – 90 %，菌相結果顯示菌群由有機物降解菌演替為以Candidatus Brocadia為主之ANAMMOX群落，AC3反應槽ANAMMOX豐度最高達8.2 %。 懸浮態菌群之厭氧氨氧化菌比活性(Specific ANAMMOX Activity, SAA)為1.33 mg-N/ mg-VSS-day。固定化方面，以PVA-PEGDA/ Light材料列印所得載體，在總氮濃度為200 mg-N/ L下SAA可達1.51 mg-N/ mg-VSS-day，優於懸浮態。保存測試顯示，室溫厭氧條件(S3)保存10天後SAA維持1.55 mg-N/ mg-VSS-day，無需冷藏或添加保護劑，展現固定化技術之操作便利性與應用潛力。 | zh_TW |
| dc.description.abstract | Nitrogen pollution is a common and cumulative issue in aquatic environments. While conventional nitrification–denitrification processes require high energy and external carbon, anaerobic ammonium oxidation (ANAMMOX) provides an autotrophic alternative with low carbon demand and sludge yield. However, its application is limited by the slow growth and operational sensitivity of ANAMMOX bacteria.
This study established a lab-scale ANAMMOX system using activated sludge from a landfill leachate treatment unit and a food-processing wastewater plant. Operated under gradually increasing nitrogen concentrations up to 120 mg-N/L, the system showed stable ammonium and nitrite removal from day 94. Four reactors were subsequently cultivated, achieving nitrogen removal efficiencies of 60–90%. Microbial analysis revealed a shift from heterotrophs to ANAMMOX-dominated communities, with Candidatus Brocadia reaching 8.2% in reactor AC3. Suspended sludge showed a specific ANAMMOX activity (SAA) of 1.33 mg-N/ mg-VSS-day. Carriers printed with PVA-PEGDA/Light reached 1.51 (TN = 200 mg-N/ L), and after 10 days of anaerobic storage at room temperature, retained 1.55 without refrigeration or additives, demonstrating operational feasibility. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2025-08-01T16:06:50Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2025-08-01T16:06:50Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員審定書 i
誌謝 iii 摘要 v Abstract vii 目次 ix 圖次 xiii 表次 xix 第一章 緒論 1 1.1 研究背景 1 1.2 研究動機 2 1.3 研究目的 3 第二章 文獻回顧 5 2.1 氮循環與水體氮污染 5 2.1.1 氮型態與自然循環路徑 5 2.1.2 人為活動對氮循環的擾動 7 2.1.3 廢水中氮去除的技術需求-以生物程序為例 9 2.2 脫氮相關微生物及其功能 13 2.2.1 氨氧化菌(AOB)與硝酸鹽氧化菌(NOB) 13 2.2.2 異營與自營特性之脫硝菌 14 2.2.3 厭氧氨氧化菌(ANAMMOX)的潛能與限制 15 2.2.4 新興微生物路徑：n-DAMO 與硫脫氮等 17 2.3 ANAMMOX反應機制與菌群特性 20 2.3.1 ANAMMOX的生理與代謝路徑 20 2.3.2 常見ANAMMOX菌屬與其特徵 21 2.3.3 培養條件與富集限制因素 23 2.3.4 ANAMMOX在不同系統中的應用案例 24 2.4 微生物固定化技術在廢水處理中的應用 26 2.4.1 微生物固定化技術類型、選擇與優缺點 26 2.4.2 微生物固定化對菌體活性與穩定性的影響 29 2.4.3 固定化ANAMMOX的應用現況與挑戰 29 2.5 3D生物列印技術概述 31 2.5.1 3D生物列印的基本原理與分類 31 2.5.2 微生物應用中材料特性與列印參數對微生物生存影響 34 2.5.3 3D列印於固定化微生物的應用案例與研究進展 34 2.6 高通量基因定序技術在微生物研究中的應用 36 2.6.1 高通量基因定序技術簡介(NGS與TGS) 36 2.6.2 高通量定序在脫氮系統微生物群落分析中的應用 37 第三章 材料與方法 41 3.1 研究流程 41 3.2 實驗藥品與設備 43 3.2.1 實驗用藥品 43 3.2.2 實驗用儀器設備 46 3.3 厭氧氨氧化菌(ANAMMOX bacteria)之培養條件與鑑定 47 3.3.1 污泥來源 47 3.3.2 厭氧氨氧化菌培養之合成廢水組成與反應條件設定 49 3.3.3 運行期間之定期監測與分析 52 3.3.4 微生物群落結構分析:PacBio全長16S rRNA定序技術解析菌相變化 54 3.4 厭氧氨氧化菌比活性分析(Specific ANAMMOX Activity, SAA) 57 3.4.1 氨氮(NH3-N)濃度測定方法 58 3.4.2 亞硝氮(NO2⁻-N)與硝酸氮(NO3⁻-N)濃度測定方法 60 3.4.3 厭氧氨氧化菌比活性(SAA)之計算與表示 61 3.5 微生物固定化與3D列印設計 63 3.5.1 3D生物列印機及列印參數 63 3.5.2 固定化材料生物墨水配方製備 64 3.5.3 生物墨水材料列印步驟 66 3.5.4 固定化後載體之處理與活化 68 3.6 最佳固定化載體與懸浮態ANAMMOX菌比活性(SAA)比較分析 69 3.6.1 氮源濃度漸增下之生物固定化載體反應適應性測試 69 3.6.2 生物固定化載體保存穩定性測試 70 3.7 固定化ANAMMOX載體之特性分析 72 3.7.1 載體微結構觀察與分析：了解固定化形貌與孔隙結構 72 3.7.2 不同階段菌體生長狀態觀察：以螢光染色評估菌體活性 73 第四章 結果與討論 77 4.1 厭氧氨氧化菌培養與反應系統運行分析 77 4.1.1 培養啟動過程與初期系統表現 77 4.1.2 培養過程之氮源濃度變化與系統穩定性分析 80 4.1.3 定期監測參數統整與趨勢探討 88 4.2 微生物群落結構變化分析 96 4.2.1 微生物多樣性變化(Alpha & Beta Diversity) 96 4.2.2 ANAMMOX系統運行期間菌相組成變化 100 4.2.3 ANAMMOX系統運行期間ANAMMOX 群落相對豐度分析 113 4.3 懸浮態污泥中ANAMMOX菌群活性評估(SAA測試) 120 4.3.1 氨氮與亞硝氮去除效率分析 120 4.3.2 SAA 計算結果與活性趨勢評估 124 4.4 微生物固定化與3D列印之應用成果 125 4.4.1 列印參數設定與製程穩定性觀察 125 4.4.2 墨水與材料配方對列印成品的影響 129 4.4.3 固定化載體培養條件之適用性分析 131 4.5 固定化與懸浮態 ANAMMOX 菌之活性比較分析 138 4.5.1 氮源濃度漸增下之活性反應與耐受性分析 138 4.5.2 固定化載體之保存穩定性與活性維持效果探討 141 4.6 固定化載體之結構與菌體生長狀態分析 148 4.6.1 載體微結構觀察(SEM)結果與孔隙性探討 148 4.6.2 載體內部菌體生長狀態螢光染色結果分析 151 第五章 結論與建議 159 5.1 結論 159 5.2 建議 161 參考文獻 163 附錄 173 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | ANAMMOX | zh_TW |
| dc.subject | 厭氧氨氧化比活性 | zh_TW |
| dc.subject | 菌相分析 | zh_TW |
| dc.subject | 除氮 | zh_TW |
| dc.subject | 微生物固定化 | zh_TW |
| dc.subject | 3D生物列印 | zh_TW |
| dc.subject | Microbial Community Analysis | en |
| dc.subject | ANAMMOX | en |
| dc.subject | 3D Bioprinting | en |
| dc.subject | Microbial Immobilization | en |
| dc.subject | Nitrogen Removal | en |
| dc.subject | Specific Anammox Activity | en |
| dc.title | 應用3D生物列印於ANAMMOX菌固定化材料開發與效能評估之研究 | zh_TW |
| dc.title | Development and Functional Assessment of ANAMMOX Immobilization Materials Using 3D Bioprinting | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 113-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 黃郁慈;張怡塘 | zh_TW |
| dc.contributor.oralexamcommittee | Yu-Tzu Huang;Yi-Tang Chang | en |
| dc.subject.keyword | ANAMMOX,厭氧氨氧化比活性,菌相分析,除氮,微生物固定化,3D生物列印, | zh_TW |
| dc.subject.keyword | ANAMMOX,Specific Anammox Activity,Microbial Community Analysis,Nitrogen Removal,Microbial Immobilization,3D Bioprinting, | en |
| dc.relation.page | 193 | - |
| dc.identifier.doi | 10.6342/NTU202501555 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2025-07-28 | - |
| dc.contributor.author-college | 工學院 | - |
| dc.contributor.author-dept | 環境工程學研究所 | - |
| dc.date.embargo-lift | 2025-08-02 | - |
| 顯示於系所單位： | 環境工程學研究所 | |
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