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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/97625完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳穎練 | zh_TW |
| dc.contributor.advisor | Ying-Lien Chen | en |
| dc.contributor.author | 戴于翔 | zh_TW |
| dc.contributor.author | Yu-Hsiang Tai | en |
| dc.date.accessioned | 2025-07-09T16:07:23Z | - |
| dc.date.available | 2025-07-10 | - |
| dc.date.copyright | 2025-07-09 | - |
| dc.date.issued | 2025 | - |
| dc.date.submitted | 2025-06-26 | - |
| dc.identifier.citation | 安寶貞、蔡志濃、林筑蘋。2021。植物誘導性抗病。載於謝廷芳、安寶貞、林筑蘋(主編)，作物病害之非農藥防治實務專書(頁57-64)。台中市：行政院農業委員會農業試驗所。
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K., Jęcz, T., Ciereszko, I., & Kononowicz, A. K. 2023. Jasmonic acid as a mediator in plant response to necrotrophic fungi. Cells 12(7):1027. Mandal, S., Mitra, A., & Mallick, N. 2008. Biochemical characterization of oxidative burst during interaction between Solanum lycopersicum and Fusarium oxysporum f. sp. lycopersici. Physiological and Molecular Plant Pathology 72:56-61. Maurya, S., Dubey, S., Kumari, R., & Verma, R. 2019. Management tactics for fusarium wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici (Sacc.): A review. Management 4(5):1-7 McDonald, A. E., Grant, B. R., & Plaxton, W. C. 2001. Phosphite (phosphorous acid): Its relevance in the environment and agriculture and influence on plant phosphate starvation response. Journal of Plant Nutrition 24(10):1505-1519. Molinari, S., Fanelli, E. & Leonetti, P. 2014. Expression of tomato salicylic acid (SA)-responsive pathogenesis-related genes in Mi-1-mediated and SA-induced resistance to root-knot nematodes. Molecular Plant Pathology 15:255-264. Pérez-Zavala, F. G., Ojeda-Rivera, J. O., Herrera-Estrella, L., & López-Arredondo, D. 2024. Beneficial Effects of Phosphite in Arabidopsis thaliana mediated by activation of ABA, SA, and JA biosynthesis and signaling pathways. Plants 13(13):1873. Pilbeam, R. A., Howard, K., Shearer, B. L., & Hardy, G. E. St. J. 2011.Phosphite stimulated histological responses of Eucalyptus marginata to infection by Phytophthora cinnamomi. Trees 25:1121-1131. Safaie-Farahani, A & Taghavi, S. M. 2017. Transcript analysis of some defense genes of tomato in response to host and non-host bacterial pathogens. Molecular Biology Research Communications 6:177-183. Saindrenan, P., Barchietto, T., Avelino, J., & Bompeix, G. 1988. Effects of phosphite on phytoalexin accumulation in leaves of cowpea infected with Phytophthora cryptogea. Physiological and Molecular Plant Pathology 32(3):425-435. Silva, J. A. G., Resende, M. L. V., Monteiro, A. C. A., Pádua1, M. A., Guerra-Guimarães, L., Fernanda L. Medeiros, F. L., Stéfanny A. Martins, S. A., & Botelho1, D. M. S. 2019. Resistance inducers applied alone or in association with fungicide for the management of leaf rust and brown eye spot of coffee under field conditions. Journal of Phytopathology 167:430-439. Singh, V. K., Singh, H. B., & Upadhyay, R. S. 2017. Role of fusaric acid in the development of 'Fusarium wilt' symptoms in tomato: Physiological, biochemical and proteomic perspectives. Plant Physiology and Biochemistry 118:320-332. Upadhyay, R. K., & Mattoo, A. K. 2018. Genome-wide identification of tomato (Solanum lycopersicum L.) lipoxygenases coupled with expression profiles during plant development and in response to methyl-jasmonate and wounding. Journal of Plant Physiology 231:318-328. Vinas, M., Mendez, J. C., & Jiménez, V. M. 2020. Effect of foliar applications of phosphites on growth, nutritional status and defense responses in tomato plants. Scientia Horticulturae 265:109200. Walker, G. E. 1989. Phytotoxicity in mandarins caused by phosphorous acid. Australasian Plant Pathology 18:57-59. Wicks, T., & Hall, B. 1990. Evaluation of phosphonic (phosphorous) acid for the control of Phytophthora cambivora on almond and cherry in South Australia. Australasian Plant Pathology 19:132-133. Yogev, E., Sadowsky, A., Solei, Z., Oren, Y., & Orbach, Y. 2006. The performance of potassium phosphite for controlling Alternaria brown spot of citrus fruit. Journal of Plant Diseases and Protection 113(5):207-213. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/97625 | - |
| dc.description.abstract | 番茄為世界上最重要的經濟作物之一，然而在栽培過程中面臨多種病害的侵襲，其中由土媒性病原真菌Fusarium oxysporum f. sp. lycopersici引起的番茄萎凋病於台灣長期缺乏推薦的防治藥劑，僅能依靠抗病品種與耕作防治等其他手段。亞磷酸為國內「免登記植物保護資材」之一，用於防治多種作物的卵菌類病害已行之有年，雖然其抗病機制尚未被完全了解，但多數認為與誘發植物防禦反應有關。本研究透過in vitro實驗與盆栽實驗測試亞磷酸防治番茄萎凋病的能力，並利用qPCR分析三個番茄根部的防禦相關基因於亞磷酸處理後的表現量變化，以試圖找出亞磷酸的抗病作用機制。In vitro實驗結果顯示亞磷酸於低濃度下對番茄萎凋病菌無顯著的直接抑菌或殺菌效果，且最低抑菌濃度為3.2%，遠大於本研究測試使用的田間推薦濃度0.2% (葉面噴灑)與0.5% (土壤澆灌)，但兩個推薦濃度於盆栽實驗中皆出現顯著的防治效果。qPCR分析則顯示僅澆灌處理組的部分基因表現量則有短時間地顯著上調，但無長時間維持，因此推測亞磷酸對番茄萎凋病主要機制可能為防禦預警效應(priming)。綜上所述，本研究發現亞磷酸具有防治番茄萎凋病的潛力，並期望與現行防治策略搭配使用能進一步減少病害所造成的損害。 | zh_TW |
| dc.description.abstract | Tomato is one of the most important vegetable crops in the world. However, its production is threatened by various pests and diseases, including Fusarium wilt, caused by the soil-borne pathogen Fusarium oxysporum f. sp. lycopersici. In Taiwan, there are no recommended fungicides for managing this disease, leaving farmers to rely on resistant cultivars and cultural control methods. Phosphonic acid has been used to control oomycete diseases in various crops for decades. Although its mode of action is not fully understood, it is believed to induce plant defense responses. This study investigates the effectiveness of phosphonic acid against tomato Fusarium wilt through in vitro assays and pot experiments. Additionally, the expression of three defense-related genes in tomato roots was evaluated using quantitative PCR (qPCR). The in vitro tests revealed that phosphonic acid had little to no antifungal activity against F. oxysporum f. sp. lycopersici, with a minimum inhibitory concentration of 3.2%, much higher than the recommended field application rates (0.2% for foliar spray and 0.5% for soil-drench), both of which were used in this study. Despite this, phosphonic acid application significantly reduced disease severity in pot assays. qPCR analysis indicated that only the soil-drench treatment led to a temporary but significant upregulation of gene expression. These findings suggest that the effectiveness of phosphonic acid in controlling Fusarium wilt may result from the priming of plant defense responses. This study presents a potential supplementary method for managing tomato Fusarium wilt, aiming to improve disease control without chemical fungicides. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2025-07-09T16:07:23Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2025-07-09T16:07:23Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 中文摘要 i
英文摘要 ii 目次 iii 表次 iv 圖次 v 壹、前言 1 貳、材料與方法 5 一、 實驗菌株之培養與保存 5 二、 番茄萎凋病菌厚膜孢子液製備 5 三、 亞磷酸溶液配製 6 四、 濾紙片擴散試驗 6 五、 含藥培養基試驗 6 六、 亞磷酸對番茄萎凋病菌分生孢子萌芽與菌絲生長影響試驗 6 七、 最低抑菌濃度與最低殺菌濃度試驗 7 八、 實驗植物之品種與種植 7 九、 亞磷酸對番茄萎凋病之防治試驗 7 十、 測試亞磷酸對蕃茄防禦相關基因的影響 8 十一、 數據統計及分析 9 參、結果 10 一、 亞磷酸於低濃度下對番茄萎凋病菌無顯著的抑制或殺菌效果 10 二、 亞磷酸處理能提高番茄植株對番茄萎凋病菌的抵抗能力 10 三、 亞磷酸能促進部分番茄根部防禦相關基因的表現 11 肆、討論 13 伍、參考文獻 18 陸、圖表集 24 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 亞磷酸 | zh_TW |
| dc.subject | 防禦預警效應 | zh_TW |
| dc.subject | 免登記植物保護資材 | zh_TW |
| dc.subject | 番茄萎凋病菌 | zh_TW |
| dc.subject | 番茄 | zh_TW |
| dc.subject | Tomato | en |
| dc.subject | priming | en |
| dc.subject | plant protection products exempted from registration | en |
| dc.subject | phosphonic acid | en |
| dc.subject | Fusarium oxysporum f. sp. lycopersici | en |
| dc.title | 亞磷酸作為番茄萎凋病防治資材之潛力 | zh_TW |
| dc.title | Phosphonic acid as a potential treatment for controlling tomato Fusarium wilt | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 113-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 鍾嘉綾;曾敏南 | zh_TW |
| dc.contributor.oralexamcommittee | Chia-Lin Chung;Min-Nan Tseng | en |
| dc.subject.keyword | 番茄,番茄萎凋病菌,亞磷酸,免登記植物保護資材,防禦預警效應, | zh_TW |
| dc.subject.keyword | Tomato,Fusarium oxysporum f. sp. lycopersici,phosphonic acid,plant protection products exempted from registration,priming, | en |
| dc.relation.page | 36 | - |
| dc.identifier.doi | 10.6342/NTU202501265 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2025-06-26 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 植物醫學碩士學位學程 | - |
| dc.date.embargo-lift | 2025-07-10 | - |
| 顯示於系所單位： | 植物醫學碩士學位學程 | |
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