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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李勇毅 | zh_TW |
| dc.contributor.advisor | Yung-I Lee | en |
| dc.contributor.author | 楊梓立 | zh_TW |
| dc.contributor.author | Tzu-Li Yang | en |
| dc.date.accessioned | 2025-02-25T16:24:03Z | - |
| dc.date.available | 2025-02-26 | - |
| dc.date.copyright | 2025-02-25 | - |
| dc.date.issued | 2025 | - |
| dc.date.submitted | 2025-02-13 | - |
| dc.identifier.citation | 王瑞閔（2018）冰淇淋必備-香草蘭。載於王瑞閔（編），看不見的雨林—福爾摩沙兩林植物誌（180 – 190頁）。麥浩斯出版。
呂毓真（2010）。環境因子對文心蘭光合作用及 Rubisco 活性之影響。博士論文，國立臺灣大學園藝暨景觀學系。 宋品慧（2021）。營農型光電綠能設施下香莢蘭最佳栽培介質. 高雄區農業改良場年報，109，29-30. 林宜樺、葉志新（2022）。香莢蘭栽培管理技術。桃園區農技報導，87，1-4。 洪丁楨（2012）。蝴蝶蘭光合作用研究—生理試驗與生物晶片綜合分析。碩士論文，國立臺灣大學園藝暨景觀學系。 海關進出口統計（2024）。貨品編號: 0905100000 香草，未壓碎或未研磨者；0905200000 香草，壓碎或研磨者。上網日期：2024年12月24日。檢自：https://portal.sw.nat.gov.tw/APGA/GA30 張能义、李存信（1991）。不同季節中香莢蘭葉片的光合作用特徵。雲南植物研究，13(2)，170-180。 陳怡安（2014）。溫度及光強度處理對臺灣白花蝴蝶蘭 (Phalaenopsis aphrodite subsp. formosana) 組培苗光合型態轉換與生理生長之影響。碩士論文，國立屏東科技大學農園生產系。 葉志新（2021）。香莢蘭果莢發育，微體繁殖及調製之研究。博士論文，國立臺灣大學農藝學系。 Amin, A. B., Rathnayake, K. N., Yim, W. C., Garcia, T. M., Wone, B., Cushman, J. C., & Wone, B. W. M. (2019). Crassulacean acid metabolism abiotic stress-responsive transcription factors: A potential genetic engineering approach for improving crop tolerance to abiotic stress. Frontiers in Plant Science. 10, 129. https://doi.org/10.3389/fpls.2019.00129 Barker, D. H., Seaton, G. G. R., & Robinson, S. A. (1997). 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/96995 | - |
| dc.description.abstract | Vanilla planifolia Andrews (香莢蘭)為景天酸代謝(crassulacean acid metabolism， CAM)的攀附性蘭花，其果莢為世界上最具有價值的香料作物之一。自香莢蘭被引 進台灣以來，針對其栽培技術的研究逐漸增多，但關於其生態生理學的相關研究仍 然有限。本研究旨在測量香莢蘭在不同葉片發育階段以及不同生長季節與遮陰度 下的光合作用特性。研究中量測了香莢蘭葉片的氣體交換、葉綠素螢光、蘋果酸濃 度、phosphoenolpyruvate carboxylase (PEPCase)活性、碳穩定同位素值(δ13C)、葉片 性狀和 PEPCase 以及 PEPCase kinase 的基因表現。
結果顯示，成熟香莢蘭葉片於中午開始固定二氧化碳直到次日清晨。兩種 PEPCase 蛋白異構體的轉錄體皆不具有明顯晝夜變化，而 PEPCase 活性在白天可 能受 PEPCase kinase 調控。在幼葉(第一片葉)的發育過程，發現香莢蘭光合作用途 徑從 C3 轉變成 CAM。幼葉只在白天固定二氧化碳，顯示其為 C3 途徑。然而葉片 厚度和液胞大小顯示幼葉已具備 CAM 的特徵。關於幼葉的 CAM 相關基因調控值 得後續釐清。在桃園區農業改良場的田間實驗，不同季節的實驗結果顯示在夏季生 長的成熟葉片(第八片葉)表現較高的光合作用能力。而在夏季三種光強度的遮陰實 驗，相較於 1.7 mol photons m-2 d-1 的光強度，生長於 6.7 和 3.8 mol photons m-2 d-1 的成熟葉片具有較高的光合作用能力，而三種光條件下葉片的 Fv/Fm 無顯著差異。 本論文提供香莢蘭不同成熟度葉片、不同季節以及遮陰條件下的光合作用數據， 可作為台灣香莢蘭產業栽培應用之參考。 | zh_TW |
| dc.description.abstract | Vanilla planifolia Andrews is a climbing orchid with crassulacean acid metabolism (CAM), and its beans are among the most valuable spice crops in the world. Since its introduction to Taiwan, research has focused on cultivation, yet studies on its ecophysiology remain limited. This study aimed to investigate the photosynthetic characteristics of V. planifolia at different developmental stages and grown under different environmental conditions. In study thesis, gas exchange, chlorophyll fluorescence, malate concentration, phosphoenolpyruvate carboxylase (PEPCase) activity, stable carbon isotope ratios (δ13C), leaf traits, and the gene expression of PEPCase and PEPCase kinase were measured.
Results showed that mature V. planifolia leaves exhibited CO2 fixation from noon, continuing until the following morning. Both the gene expression of PEPCase isoforms exhibited no daily fluctuation, while PEPCase activity may be regulated by PEPCase kinase during the daytime. During the development of young leaves, the photosynthetic transition from C3 to CAM pathway was observed. Young leaves (the 1st leaves) exhibited only diurnal CO2 fixation, indicated a predominant C3 pathway. However, leaf thickness and vacuole size revealed that young leaves possessed structural features associated with CAM. The regulation of CAM-related genes requires further investigation. In field experiments at Taoyuan District Agricultural Research and Extension Station, mature leaves (the 8th leaf) of V. planifolia grown in summer exhibited higher photosynthetic capacity compared to those in other seasons. In the shading experiments under three light intensities during summer, mature leaves grown under 6.7 and 3.8 mol photons m-2 d-1 showed higher photosynthetic capacity compared to those grown under 1.7 mol photons m-2 d-1. However, no significant differences were observed in Fv/Fm among the three light conditions. This thesis provides photosynthetic data of V. planifolia leaves at different developmental stages, across seasons, and under various shading conditions, serving as a valuable reference for the cultivation and management of V. planifolia in Taiwan. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2025-02-25T16:24:03Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2025-02-25T16:24:03Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員會審定書 #
誌謝 i 摘要 ii ABSTRACT iii CONTENTS v LIST OF FIGURES viii LIST OF TABLES xiii Chapter 1 Introduction 1 Chapter 2 Literature Review 3 2.1 Introduction of Vanilla 3 2.1.1 Cultivation History of Vanilla 3 2.1.2 Global Market Overview of Vanilla 3 2.1.3 Morphological Characteristics of V. planifolia 4 2.2 Crassulacean Acid Metabolism (CAM) 5 2.2.1 Characteristics of CAM 5 2.2.2 Evolution and Transition from C3 to CAM 8 2.2.3 PEPCase and PEPCase Kinase in CAM 9 2.3 Effects of Developmental Stages on Photosynthesis 10 2.4 Effects of Different Environments on Photosynthesis 12 Chapter 3 Materials and Methods 15 3.1 Plants Materials 15 3.2 Gas Exchange Measurement 16 3.2.1 Assimilation Rates Over a Whole Day 16 3.2.2 Photosynthesis Light Response Curve Measurement 16 3.3 Chlorophyll Fluorescence Measurement 17 3.4 Biochemical Measurement 18 3.4.1 Malate Concentration Measurement 18 3.4.2 PEPCase Activity Measurement 19 3.5 Stable Carbon Isotope Ratios (δ13C) 20 3.6 RNA Isolation and Gene Expression Analysis 21 3.6.1 Sequence Information on V. planifolia 21 3.6.2 RNA Extraction and Reverse Transcription 21 3.6.3 PCR Experiments 22 3.7 Total Soluble Sugar and Starch Contents Measurement 22 3.8 Morphological and Histological Studies 24 3.8.1 Morphological Trait Measurement 24 3.8.2 Free-Hand Sectioning and Staining 24 3.8.3 Histological Study 25 3.8.4 Protoplast Isolation and Staining 25 3.9 Experimental Design 26 3.9.1 The Photosynthetic Variation Over a Whole Day 26 3.9.2 The Difference of Photosynthetic Pathway in Leaves at Different Nodes 27 3.9.3 The Difference of Photosynthetic Pathway in Leaves at Various Developmental stages 28 3.9.4 The Photosynthetic Variation across Four Seasons 28 3.9.5 The Photosynthetic Variation under Different Shadings 29 3.10 Statistical analysis 29 Chapter 4 Results 32 4.1 Photosynthetic Variation in V. planifolia Leaves Over a Whole Day 32 4.2 Photosynthetic Variation in V. planifolia Leaves at Different Nodes 33 4.2.1 Principle Component Analysis 33 4.2.2 The Stable Carbon Isotope Ratio of V. planifolia Leaves at Various Nodes 34 4.3 Photosynthetic Variation in V. planifolia during Leaf Development 34 4.4 Morphology and Histology of V. planifolia Leaves 35 4.4.1 Morphological and Histological Developments of Leaves at Various Nodes 35 4.4.2 Morphological and Histological Developments of V. planifolia Leaves in Developmental Stage 36 4.5 Photosynthetic Variation in V. planifolia Leaves across Four Seasons 36 4.6 Photosynthetic Variation in V. planifolia under Different Shadings 38 Chapter 5 Discussion 70 5.1 Daily Photosynthetic Variation in V. planifolia 70 5.2 Photosynthetic Pathway in V. planifolia Leaves at Various Nodes 72 5.3 Photosynthetic Pathway Transition from C3 to CAM in V. planifolia During Leaf Development 73 5.4 Photosynthetic Variation in the 8th Leaves of V. planifolia across Seasons and Shading Treatments 75 Chapter 6 Conclusion 78 REFERENCE 79 APPENDIX 90 | - |
| dc.language.iso | en | - |
| dc.title | 香莢蘭在不同生長環境與發育階段的光合作用特性研究 | zh_TW |
| dc.title | Effects of Different Environments and Developmental Stages on Photosynthesis of Vanilla planifolia | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 113-1 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 高文媛;賴宜鈴;葉志新 | zh_TW |
| dc.contributor.oralexamcommittee | Wen-Yuan Kao;I-Ling Lai;Chih-Hsin Yeh | en |
| dc.subject.keyword | 蘭花,景天酸代謝(CAM),C3,氣體交換,PEPCase,蘋果酸,碳穩定同位素值,光強度, | zh_TW |
| dc.subject.keyword | orchid,crassulacean acid metabolism (CAM),C3,gas exchange,phosphoenolpyruvate carboxylase (PEPCase),malate,stable carbon isotope ratio,light intensity, | en |
| dc.relation.page | 101 | - |
| dc.identifier.doi | 10.6342/NTU202500475 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2025-02-13 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 生命科學系 | - |
| dc.date.embargo-lift | 2027-03-01 | - |
| 顯示於系所單位： | 生命科學系 | |
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| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-113-1.pdf 此日期後於網路公開 2027-03-01 | 21.33 MB | Adobe PDF |
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