請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/96678完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 姚宗珍 | zh_TW |
| dc.contributor.advisor | Chung-Chen Jane Yao | en |
| dc.contributor.author | 徐儷芳 | zh_TW |
| dc.contributor.author | Li-Fang Hsu | en |
| dc.date.accessioned | 2025-02-20T16:29:52Z | - |
| dc.date.available | 2025-02-21 | - |
| dc.date.copyright | 2025-02-20 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-10-22 | - |
| dc.identifier.citation | 參考文獻
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Mechanical activation of lung epithelial cells through the ion channel Piezo1 activates the metalloproteinases ADAM10 and ADAM17 and promotes growth factor and adhesion molecule release. Biomater Adv. Sep 2023;152:213516. doi:10.1016/j.bioadv.2023.213516 | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/96678 | - |
| dc.description.abstract | 鼻塞在發育階段對呼吸系統和顱顏顎骨形態產生了相當大的生理影響。本研究使用Mmp-3-luc基因轉殖大鼠進行活體冷光影像追踪，在單側鼻塞後長期觀察大鼠鼻部區域的表達。通過顯微斷層掃描檢查和三維圖像處理，計算對顱面部、鼻部和鼻竇區域的骨骼變化進行了測量和分析。還通過免疫組織化學（IHC）檢查了基質金屬蛋白酶-3和嗅覺標記蛋白的表達情況。實驗結果顯示單側鼻塞顯著降低了Mmp-3-luc轉基因大鼠鼻部區域的Mmp-3信號；而此訊號主要表達在呼吸上皮中。長期阻塞還導致了顱面部骨組織的形態學變化，如鼻中膈偏移、較長的上下顎間距和增加的上顎臼齒齒槽高度。免疫組織化學結果證實，它還導致了嗅覺神經束和嗅覺上皮的代償性生長。我們也利用Mmp-3-luc基因轉殖大鼠鼻黏膜上皮細胞之初代培養來測試培養基流動形成之剪力對nasoepithelial cell的影響，經由免疫螢光染色發現主要Luciferase之陽性染色在類似間質細胞樣態的細胞群而非末端分化上皮細胞樣態中。
在我們的研究中，長期的單側鼻塞導致鼻中膈向未受阻力的一側偏移，上顎臼齒高度發育變化，並減少了呼吸上皮中MMP-3的產生。然而，詳細的機制仍需要進一步的研究來探究。 | zh_TW |
| dc.description.abstract | Nasal obstruction exerts considerable physiological effects on the respiratory system and craniofacial morphology during the developmental stage. In this study, the author used Mmp-3-luc transgenic rats for in vivo tracking of long-term expression of Mmp-3 mRNA in the rat nasal region after unilateral nasal obstruction. Skeletal changes of the craniofacial, nasal, and sinus regions were measured and calculated through micro-computed tomography examination and analysis with 3D image processing software. Matrix metalloproteinase-3 and olfactory marker protein expressions were also investigated through immunohistochemistry (IHC) staining. In the result, unilateral nasal obstruction significantly reduced the MMP-3 signal in the nasal region of Mmp-3-luc transgenic rats, which was mainly expressed in the respiratory epithelium. Long-term obstruction also caused morphological changes of the craniofacial hard tissue, such as nasal septal deviation, longer inter-jaw distance, and increased maxillary molar dental height. It also caused compensatory growth in olfactory nerve bundles and the olfactory epithelium, as confirmed by IHC. In our study, long-term unilateral nasal obstruction caused nasal septal deviation toward the unobstructed side, hyperdivergent facial development including longer molar dental height, and reduced MMP-3 production. For the in-vitro experiment, luciferase staining through immunofluorescence showed positive staining after shear stress flow experiment. However, further investigation is necessary to explore the mechanism in depth. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2025-02-20T16:29:52Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2025-02-20T16:29:52Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員會審定書 1
誌謝 2 中文摘要 3 英文摘要 4 第一章 緒論 11 1.1 長期鼻塞對靈長類動物與人類顱顏顎骨發育的影響 11 1.2 長期鼻塞對顱顏骨發育的影響—以囓齒類動物為模型 12 1.3 囓齒類動物鼻阻塞方式之比較 13 1.4 長期鼻塞對於嗅覺之影響 14 1.5 基質金屬蛋白酶(matrix metalloproteinases, MMPs)對骨骼重塑之影響 15 1.6呼吸氣流，呼吸上皮與基質金屬蛋白酶-3表現的相關性 16 第二章 實驗目的 18 第三章 實驗方法 19 3.1 攜帶Mmp-3人類3.2kb啟動子-LUC報導基因的基因轉殖大鼠模型建立 19 3.2 基因轉殖大鼠注射冷光素後之藥物動力測試 19 3.3 Mmp-3-luc基因轉殖大鼠之鼻阻塞實驗設計與活體冷光影像系統(IVIS, In-vivo imaging system)用於追蹤MMP-3表達 20 3.4阻塞鼻腔之印模材料長期於溫濕環境下之穩定度測試 21 3.5 Micro computerized tomography微米電腦斷層掃描 22 3.6 Amira三維影像資料處理平台分析顱顏與鼻腔發育 22 3.7 切片與免疫化學染色: MMP-3、LUC和嗅覺標記蛋白(olfactory marker protein)進行免疫組織化學染色 24 3.8 鼻黏膜細胞: 初代培養與使用人類細胞株 24 3.9流體剪切力系統實驗與免疫螢光染色 25 3.10統計分析 27 第四章 實驗結果 28 4.1基因轉殖大鼠注射冷光素後之藥物動力測試 28 4.2 Mmp-3-luc基因轉殖大鼠之鼻阻塞實驗設計與活體冷光影像系統(IVIS, In-vivo imaging system)用於追蹤MMP-3表達 29 4.3阻塞鼻腔之印模材料長期於溫濕環境下之穩定度測試 34 4.4 Amira三維影像資料處理平台分析顱顏與鼻腔發育 35 4.4.1 長期單側鼻阻塞對上顎竇腔發育之影響 35 4.4.2 長期單側鼻阻塞對上顎發育長度、寬度與高度之影響 36 4.4.3 長期單側鼻阻塞對上顎鼻中膈彎曲角度影響 39 4.5 組織學切片與免疫化學染色 40 4.6鼻黏膜細胞之初代培養、流體剪切力系統實驗與免疫螢光染色 43 4.6.1 初代培養結果由組織外植法與酵素萃取法之比較 43 4.6.2流體剪切力系統實驗與免疫螢光染色 44 第五章 討論 46 5.1 材料穩定性測試與鼻孔阻塞方法對生理影響層面討論 46 5.2 單側鼻阻塞對上顎竇生長體積變化之影響 46 5.3 嚙齒類動物作為專性鼻呼吸動物是否能習得口呼吸 47 5.4 大鼠顱顏顎骨發育受到單側鼻阻塞之影響 47 5.5 單側鼻阻塞對鼻腔發育與鼻中膈彎曲角度之影響 48 5.6 單側鼻阻塞對MMP-3於呼吸上皮表現與流體剪切力系統實驗對鼻黏膜上皮細胞在Luciferase表達之影響 48 5.7單側鼻阻塞對嗅覺標記蛋白於嗅覺上皮與鼻腔表現之影響與推論 49 5.8流體剪切力系統實驗(ibidi cell pump) 對鼻黏膜上皮細胞在Luciferase免疫組織螢光染色的表達之影響 50 5.9機械力轉換成細胞內訊息傳導訊號之機制探討 50 第六章 本研究之限制與未來方向 52 第七章 結論 53 附錄一 活體冷光追蹤訊號照片 54 附錄二 組織學切片與免疫組織化學染色照片 64 附錄三 SAS統計分析結果 78 附錄四 中英文縮寫對照表 89 參考文獻 90 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 微米斷層掃描 | zh_TW |
| dc.subject | 鼻阻塞 | zh_TW |
| dc.subject | 基質金屬蛋白酶三 | zh_TW |
| dc.subject | 活體影像追蹤系統 | zh_TW |
| dc.subject | nasal obstruction | en |
| dc.subject | in-vivo imaging system | en |
| dc.subject | microCT | en |
| dc.subject | MMP-3 | en |
| dc.title | 長期單側鼻阻塞對顱顏骨與嗅覺神經發育之影響—以冷光標定基質金屬蛋白酶-3基因啟動子之基因轉殖大鼠為模型 | zh_TW |
| dc.title | Craniofacial and Olfactory Sensory Changes after Long-term Unilateral Nasal Obstruction—An Animal Study Using Mmp-3-luc Transgenic Rats | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 113-1 | - |
| dc.description.degree | 博士 | - |
| dc.contributor.oralexamcommittee | 葉德輝;許巍鐘;王詩凱;陳羿貞 | zh_TW |
| dc.contributor.oralexamcommittee | Te-Huei Yeh;Wei-Chung Hsu;Shih-Kai Wang;Yi-Jane Chen | en |
| dc.subject.keyword | 鼻阻塞,基質金屬蛋白酶三,微米斷層掃描,活體影像追蹤系統, | zh_TW |
| dc.subject.keyword | nasal obstruction,MMP-3,microCT,in-vivo imaging system, | en |
| dc.relation.page | 95 | - |
| dc.identifier.doi | 10.6342/NTU202404461 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2024-10-22 | - |
| dc.contributor.author-college | 醫學院 | - |
| dc.contributor.author-dept | 臨床牙醫學研究所 | - |
| dc.date.embargo-lift | 2025-02-21 | - |
| 顯示於系所單位： | 臨床牙醫學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-113-1.pdf | 12.57 MB | Adobe PDF | 檢視/開啟 |
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