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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鍾嘉綾 | zh_TW |
| dc.contributor.advisor | Chia-Lin Chung | en |
| dc.contributor.author | 許任鈞 | zh_TW |
| dc.contributor.author | Jen-Chun Hsu | en |
| dc.date.accessioned | 2025-02-13T16:19:42Z | - |
| dc.date.available | 2025-02-14 | - |
| dc.date.copyright | 2025-02-13 | - |
| dc.date.issued | 2025 | - |
| dc.date.submitted | 2025-02-08 | - |
| dc.identifier.citation | Ann, P. J., Chang, T. T., and Ko, W. H. 2002. Phellinus noxius brown root rot of fruit and ornamental trees in Taiwan. Plant Dis. 86:820-826.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/96406 | - |
| dc.description.abstract | 褐根病菌 (Phellinus noxius) 是危害樹木根莖部的重要壞死性病原菌，其引起的樹木褐根病 (brown root rot disease) 近年來嚴重危害果樹、觀賞樹木、森林及都市林木，造成經濟損失並影響公共安全，而P. noxius的致病機制仍有諸多未知。本研究以基因組預測並比對轉錄組數據，篩選出 79 個候選效應蛋白 (effectors)，其中包含 26 個 hypothetical proteins、29 個碳水化合物活性酶 (carbohydrate-active enzymes, CAZymes)、4 個 cerato-platanins (CPs) 及 3 個疏水蛋白 (hydrophobins)。進一步針對 P. noxius 基因體中 5 個 cerato-platanins (PnCPs) 的核苷酸多樣性、親緣關係及蛋白質預測結構進行分析，發現其可能受純化選擇作用，具有 CPs 特徵序列與保守結構，並具有幾丁質四聚體結合潛力。檢測不同環境條件下 PnCPs 之基因表現，發現 PnCP1 及 PnCP4 在馬鈴薯葡萄醣瓊脂培養基 (potato dextrose agar, PDA) 上菌絲生長旺盛時表現量較高，但液態完全培養基 (liquid complete medium, LCM) 下僅 PnCP4 有較高表現量。感染毛果楊 (Populus trichocarpa) 離體莖段時，PnCP1於 1 dpi (day post-infection) 之後表現量即顯著降低直至 4 dpi，可能與避免誘導植物啟動免疫反應有關；而 PnCP4 與 PnCP5 在 2 dpi 表現量顯著上調，其中 PnCP4 上調 14 倍以上，顯示兩者可能與 P. noxius 毒力有相關性。利用農桿菌浸潤法初步檢測5個 PnCPs 及其他 10 個候選效應蛋白基因之功能，在圓葉菸草 (Nicotiana benthamiana) 葉片短暫表達後皆未能誘導細胞死亡，推測這些候選效應蛋白可能有寄主或組織特異性，或具有誘導細胞死亡之外的生物活性。本研究為 P. noxius 效應蛋白的功能性研究建立重要的基礎資料，可作為未來功能性驗證與致病機制研究之參考。 | zh_TW |
| dc.description.abstract | Phellinus noxius, the causal of brown root rot disease, is a significant necrotrophic pathogen that damages the roots and stems of trees. In recent years, brown root rot disease has severely affected fruit, ornamental, forest, and urban trees, causing economic losses and posing risks to public safety. However, the pathogenic mechanisms of P. noxius remain largely unknown. This study utilized genome predictions and transcriptomic data to identify 79 candidate effectors, including 26 hypothetical proteins, 29 carbohydrate-active enzymes (CAZymes), four cerato-platanins (CPs), and three hydrophobins. Further analysis of five P. noxius cerato-platanins (PnCPs) focused on the nucleotide diversity, phylogenetic relationships, and predicted protein structures. PnCPs are likely under purifying selection. PnCPs possess CPs signature sequences and conserved structures, and have potential chitin tetramer-binding ability. Gene expression analysis under different conditions showed that PnCP1 and PnCP4 had higher expression levels during vigorous mycelial growth on potato dextrose agar (PDA), while only PnCP4 exhibited high expression in liquid complete medium (LCM). When infecting the detached stem segment of Populus trichocarpa, PnCP1 expression was significantly down-regulated after 1 dpi (day post-infection) and remained low until 4 dpi, potentially associated with avoiding the triggering of plant immune response. In contrast, PnCP4 and PnCP5 expression were significantly up-regulated at 2 dpi, with PnCP4 increasing by over 14-fold, suggesting their potential role in virulence. Preliminary functional tests using agroinfiltration-based transient expression in Nicotiana benthamiana leaves showed that five PnCPs and another 10 candidate effector genes did not induce cell death. These candidate effectors may be host- or tissue-specific, or have biological activities other than inducing cell death. This study provides crucial foundational data for functional studies of P. noxius effectors and offers valuable references for future research on their pathogenic mechanisms. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2025-02-13T16:19:41Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2025-02-13T16:19:42Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 摘要 iii Abstract iv 目次 v 表次 viii 圖次 ix 第一章 前言 1 1.1 褐根病菌 1 1.2 壞死性病原菌效應蛋白 2 1.2.1 誘導植物細胞死亡的效應蛋白 2 1.2.2 抑制植物免疫的效應蛋白 3 1.2.3 Cerato-platanin family proteins (CPs, or cerato-platanins) 4 1.3 預測與鑑定效應蛋白 5 1.3.1 根據胺基酸序列預測 5 1.3.2 農桿菌浸潤法應用於篩選效應蛋白 6 1.4 研究動機與目的 6 第二章 材料與方法 7 2.1 真菌與植物材料 7 2.1.1 P. noxius 培養條件 7 2.1.2 毛果楊培養條件 7 2.1.3 圓葉菸草培養條件 7 2.2 候選效應蛋白篩選 8 2.3 P. noxius 接種毛果楊莖段 8 2.4 P. noxius 逆境處理 9 2.5 萃取RNA與製備cDNA 10 2.5.1 萃取RNA 10 2.5.2 製備cDNA 10 2.6 基因表現量分析 11 2.6.1 qRT-PCR 11 2.6.2 基因表現量相關性分析 11 2.7 候選效應蛋白基因定序 12 2.8 PnCPs序列分析 13 2.8.1 PnCPs 核苷酸多樣性分析 13 2.8.2 PnCPs胺基酸序列比對與親緣分析 13 2.8.3 PnCPs立體結構預測與幾丁質聚合物對接模擬 14 2.9 農桿菌浸潤法 14 2.9.1 表達載體構築 14 2.9.2 ParA1 elicitin signal peptide (ParA1SP) 融合表達載體構築 15 2.9.3 圓葉菸草葉片之農桿菌浸潤處理 16 第三章 結果 17 3.1 候選效應蛋白分析 17 3.2 P. noxius 基因組中 cerato-platanins (PnCPs) 17 3.3 PnCPs 核苷酸多樣性分析 17 3.4 PnCPs 序列結構及親緣分析 18 3.5 PnCPs幾丁質結合功能預測 19 3.6 不同環境條件下PnCPs的基因表現分析 19 3.7 PnCPs 誘導細胞死亡之活性檢測 20 3.8 其他候選效應蛋白誘導細胞死亡之活性檢測 21 第四章 討論 22 4.1 P. noxius效應蛋白預測 22 4.2 PnCPs 基因表現特性 24 4.3 效應蛋白誘導細胞死亡之活性檢測 27 參考文獻 30 表 43 圖 51 附錄 69 | - |
| dc.language.iso | zh_TW | - |
| dc.title | 褐根病菌 Phellinus noxius 候選效應蛋白 cerato-platanins 之探討 | zh_TW |
| dc.title | Investigation of the candidate effector protein cerato-platanins in Phellinus noxius, the cause of brown root rot disease | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 113-1 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 劉瑞芬;蔡怡陞;張皓巽 | zh_TW |
| dc.contributor.oralexamcommittee | Ruey-Fen Liou;Isheng Jason Tsai;Hao-Xun Chang | en |
| dc.subject.keyword | 褐根病菌,效應蛋白,cerato-platanins,親緣分析,蛋白結構預測,農桿菌浸潤法, | zh_TW |
| dc.subject.keyword | Phellinus noxius,effectors,cerato-platanins,phylogenetic analysis,protein structure prediction,agroinfiltration, | en |
| dc.relation.page | 77 | - |
| dc.identifier.doi | 10.6342/NTU202500541 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2025-02-10 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 植物病理與微生物學系 | - |
| dc.date.embargo-lift | 2030-02-08 | - |
| 顯示於系所單位： | 植物病理與微生物學系 | |
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