研究AtMAPR3在植物防禦的生理作用

Abstract

Botrytis cinerea是一種全球性傳播的營養壞死性真菌，植物受到感染後會導致灰黴病的發生，能感染廣泛宿主的特性使全球經濟作物都會受到B. cinerea影響。當植物在面臨B. cinerea感染的時候，會啟動一系列的防禦機制，例如：植物賀爾蒙的分泌、程序細胞凋亡 (Programmed cell death, PCD)，其中PCD又包含了自噬作用 (Autophagy) 以及細胞凋亡 (Apoptosis)，若是成功誘導Autophagy便能防禦B. cinerea的感染。AtMAPR3是阿拉伯芥中一個功能尚未被完全釐清的蛋白質，在過去的研究中，已經提出AtMAPR3參與在自噬作用以及木質素生合成 (Lignin biosynthesis) 路徑之中。透過Microarray的資料庫分析得知阿拉伯芥在被B. cinerea感染後會誘導AtMAPR3的表現量上升，這表明AtMAPR3的功能可能和阿拉伯芥防禦病原菌有關。AtMAPR3可能通過在其參與自噬作用的特性協助清除被B. cinerea感染而死亡細胞或者促進木質素生合成增強植物細胞壁幫助植物抵擋B. cinerea。在本研究中發現，我們發現AtMAPR3表現量在野生型阿拉伯芥被B. cinerea感染後的3小時時會被誘導表現，與對照組相比，表現量有明顯的增高趨勢。根據這一結果推論AtMAPR3可能在植物早期對抗B. cinerea感染的過程中扮演重要的角色，這些發現為我們進一步理解植物的抗病機制提供了新的研究方向，並揭示了AtMAPR3在植物防禦反應中的潛在重要性。未來的研究可以進一步探討AtMAPR3的具體功能和調控機制，此外研究 AtMAPR3 與其他植物防禦基因的相互作用，將有助於全面了解植物與病原菌之間的互動，並可為農業病蟲害的防治提供新的策略和方法。

Botrytis cinerea is a globally disseminated necrotrophic fungus that causes gray mold disease in plants. Its ability to infect a wide range of hosts makes it a significant threat to global economic crops. When plants face B. cinerea infection, they activate a series of defense mechanisms, such as hormone secretion and programmed cell death (PCD). PCD includes autophagy and apoptosis; successful induction of autophagy can defend against B. cinerea infection. AtMAPR3 is a protein in Arabidopsis thaliana whose function is not yet fully understood. Previous studies have suggested that AtMAPR3 is involved in autophagy and lignin biosynthesis pathways. Analysis of microarray data revealed that AtMAPR3 expression is upregulated in Arabidopsis upon B. cinerea infection, indicating that AtMAPR3 may play a role in plant defense against pathogens. AtMAPR3 may help clear cells killed by B. cinerea infection through autophagy or enhance the plant cell wall by promoting lignin biosynthesis, thus aiding in resistance against B. cinerea. In this study, we found that AtMAPR3 gene expression is significantly induced in wild-type Arabidopsis within 3 hours of B. cinerea infection, compared to the control group. This result suggests that AtMAPR3 may play an important role in the early defense response against B. cinerea infection. These findings provide new insights into the plant disease resistance mechanism and highlight the potential importance of AtMAPR3 in plant defense responses. Future research can further explore the specific functions and regulatory mechanisms of AtMAPR3. Additionally, studying the interaction between AtMAPR3 and other plant defense genes will help in comprehensively understanding the interactions between plants and pathogens, potentially providing new strategies and methods for agricultural pest and disease control.