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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張俊哲 | zh_TW |
| dc.contributor.advisor | Chun-Che Chang | en |
| dc.contributor.author | 區嘉曦 | zh_TW |
| dc.contributor.author | Ka-Hei Simon Au | en |
| dc.date.accessioned | 2024-09-15T16:51:06Z | - |
| dc.date.available | 2024-09-16 | - |
| dc.date.copyright | 2024-09-15 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-08-12 | - |
| dc.identifier.citation | 蕭逸旻 (2018)。孤雌胎生蚜蟲之體軸形成與內共生調控對生殖細胞發育影響之研究。國立臺灣大學生物科技研究所博士論文，台北市。取自https://hdl.handle.net/11296/2ugn33
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/95696 | - |
| dc.description.abstract | 以咖啡樹 (咖啡屬，Coffea 之植物) 為食的昆蟲種類繁多，超過850種昆蟲被記錄為咖啡樹的害蟲。其中，已記錄的蚜蟲有三種，分別是桃蚜 (Myzus persicae)、棉蚜 (Aphis gossypii) 和小桔蚜 (Toxoptera aurantii)。然而，有關蚜蟲代謝咖啡因的機制，以及咖啡因對蚜蟲發育和生殖能力影響的研究仍非常匱乏。僅有的研究顯示，在人工飼料中添加0.1 mg/ml 咖啡因，能使桃蚜成蟲的產子數量減少42%，壽命縮短30%。因此，探討咖啡因如何影響蚜蟲的生殖發育，是一個重要的研究議題。 本研究旨在通過人工飼料餵食蚜蟲不同濃度的咖啡因，了解其對蚜蟲各項生理指標及卵巢發育狀況的影響，以填補目前咖啡因與蚜蟲之間研究的空白。我們選取豌豆蚜 (Acyrthosiphon pisum) 作為研究對象，在其人工飼料中添加0.00、0.10、0.12、0.14、0.16、0.18及0.20 mg/ml 咖啡因進行飼養實驗。結果發現，隨著咖啡因濃度的增加，蚜蟲的成蟲率顯著下降，發育時間顯著延長，成蟲體重和體型顯著減輕。在生殖能力方面，咖啡因導致蚜蟲繁殖能力顯著下降，甚至出現不育個體 (成蟲在未產下若蟲的情況下死亡)。解剖實驗進一步顯示，咖啡因減緩了蚜蟲卵巢的發育速度，使卵巢顯著縮小。然而，咖啡因並未減少蚜蟲卵巢中的蛋腔數量，卵巢縮小的主要原因是胚胎發育遲緩。在0.20 mg/ml 咖啡因處理下，最成熟的胚胎僅達到第15期。此外，咖啡因還導致蚜蟲卵巢出現畸形結構，胚胎無法發育甚至缺乏生殖細胞。 另外，我們對蚜蟲進行抗生素處理，探討咖啡因對去共生及正常蚜蟲的影響。即使在最低劑量的咖啡因處理下，幾乎所有去共生蚜蟲均出現不育狀況。本研究結果為咖啡因對蚜蟲的生理及生殖影響提供了重要數據，並為進一步的研究奠定了基礎。了解咖啡因對蚜蟲的生理和生殖影響，可為發展新的生物農藥提供理論依據。咖啡因作為一種天然植物殺蟲劑，具有潛力成為控制蚜蟲的重要工具。 | zh_TW |
| dc.description.abstract | Coffee plants (genus Coffea) host over 850 species of insect pests, including three documented aphid species: green peach aphid (Myzus persicae), cotton aphid (Aphis gossypii), and black citrus aphid (Toxoptera aurantii). Despite this, research on aphid caffeine metabolism and the effects of caffeine on aphid development and reproduction remains scarce. Existing studies show that adding 0.1 mg/ml of caffeine to artificial diets reduces adult M. persicae offspring by 42% and shortens lifespan by 30%. This study aims to investigate how caffeine affects aphid reproductive development to fill this research gap. We selected the pea aphid (Acyrthosiphon pisum) and conducted feeding experiments with artificial diets containing 0.00, 0.10, 0.12, 0.14, 0.16, 0.18, and 0.20 mg/ml of caffeine. Results indicate that higher caffeine concentrations significantly decrease adult aphid rates, increase development time, and reduce adult weight and size. Caffeine also significantly lowers reproductive capacity, with some individuals becoming infertile (dying without producing nymphs). Dissection revealed that caffeine slows ovarian development, causing significant size reduction without reducing oocyte numbers. The primary cause of reduced ovarian size was delayed embryonic development, with the most mature embryos reaching only the 15th stage at 0.20 mg/ml caffeine. Additionally, caffeine induced ovarian structural deformities, preventing proper embryo development and resulting in a lack of germ cells. Furthermore, antibiotic treatments showed that nearly all aposymbiotic aphids became infertile even at the lowest caffeine concentrations. This study provides essential data on caffeine's physiological and reproductive effects on aphids, laying a foundation for further research. Understanding these effects can support the development of new biopesticides, positioning caffeine as a potential natural insecticide for aphid control. | en |
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| dc.description.provenance | Made available in DSpace on 2024-09-15T16:51:06Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 誌謝 i
摘要 ii Abstract iii 表次 vii 圖次 viii 附錄 ix 第一章 前言 1-6 (一) 咖啡因的應用與功能 1-2 (二) 蚜蟲的研究獨特與重要性 2-3 (三) 蚜蟲人工飼料的發展及應用 3-5 (四) 蚜蟲與內共生菌 5-6 (五) 研究動機及目的 6 第二章 材料與方法 7-13 (一) 豌豆蚜飼養 7 (二) 豌豆蚜人工飼料及餵食儀器設置 7 (三) 咖啡因濃度測試 8 (四) 人工飼料中添加咖啡因 (caffeine treatment, 實驗簡稱CAF) 對蚜蟲發育及生殖之影響 8 (五) 去共生後咖啡因 (aposymbiotic aphids caffeine treatment, 實驗簡稱APO-CAF) 對蚜蟲發育及生殖之影響 8-9 (六) 去共生同時加入咖啡因 (rifampicin and caffeine treatment, 實驗簡稱RF-CAF) 對蚜蟲發育及生殖之影響 9 (七) 蚜蟲成蟲卵巢解剖及影像處理 9-10 (八) 蚜蟲成蟲體長、微卵管長度及蛋腔數量測量 10 (九) 微卵管封片及影像處理 10 (十) 蚜蟲胚胎細胞骨架 (纖維狀肌動蛋白，F-actin) 及細胞核 (去氧核糖核酸，deoxyribonucleic acid，DNA) 螢光染色及影像處理 10-11 (十一) ApVas1 抗體染色 11-12 (十二) 統計及分析 12-13 第三章 結果 14-24 (一) 咖啡因對蚜蟲成蟲率及若蟲發育至成蟲所需時間影響之比較 14 (二) 咖啡因對蚜蟲成蟲體長及體重影響之比較 14-15 (三) 咖啡因對成蟲繁殖能力 (產下子代數量) 影響之比較 15 (四) 咖啡因對蚜蟲成蟲卵巢發育狀況與大小影響之比較 15-16 (五) 咖啡因對蚜蟲成蟲之微卵管長度及蛋腔數量影響之比較 16-17 (六) 咖啡因飲食下不同咖啡因濃度對胚胎發育時期影響之比較 17-18 (七) 咖啡因飲食造成蚜蟲卵巢畸形結構的影響 18-19 (八) APO-CAF 處理對蚜蟲成蟲率及若蟲發育至成蟲所需時間影響之比較 19-20 (九) APO-CAF 對蚜蟲成蟲體長及體重影響之比較 20 (十) APO-CAF 對成蟲繁殖能力 (產下子代數量) 影響之比較 20-21 (十一) RF-CAF 對蚜蟲成蟲率及若蟲發育至成蟲所需時間影響之比較 21-22 (十二) RF-CAF 對蚜蟲成蟲體長、體重影響之比較 22 (十三) RF-CAF 處理對成蟲生殖能力 (產下子代數量) 影響之比較 22-23 (十四) 咖啡因對成蟲卵巢之ApVas1 抗體染色影響之比較 23-24 (十五) 咖啡因對成蟲卵巢畸形胚胎結構之ApVas1 抗體染色影響之比較 24 (十六) 成蟲卵巢中畸形胚胎結構之細胞骨架及細胞核螢光染色結果 24 第四章 討論 25-36 (一) 咖啡因飲食對豌豆蚜生長發育及繁殖能力之影響 25-29 (二) 咖啡因飲食對豌豆蚜卵巢及胚胎發育之影響 29-30 (三) 咖啡因飲食對去共生豌豆蚜生長發育及繁殖之影響 30-31 (四) 咖啡因飲食對蚜蟲造成不育及卵巢致畸作用 31-33 (五) 咖啡因飲食與生殖細胞消失之關係 33-34 (六) 本研究成果及未來可進行實驗方向 34-36 參考文獻 37-46 附表 47-56 附圖 57-68 附錄 69-80 口試提問與建議 81-96 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 人工飼料 | zh_TW |
| dc.subject | 咖啡因 | zh_TW |
| dc.subject | 豌豆蚜 | zh_TW |
| dc.subject | 胚胎發育 | zh_TW |
| dc.subject | 內共生菌 | zh_TW |
| dc.subject | 生殖能力 | zh_TW |
| dc.subject | endosymbiont | en |
| dc.subject | Pea aphids | en |
| dc.subject | Acyrthosiphon pisum | en |
| dc.subject | artificial diets | en |
| dc.subject | caffeine | en |
| dc.subject | fecundity | en |
| dc.subject | embryogenesis | en |
| dc.title | 咖啡因對孤雌胎生豌豆蚜之生殖發育影響 | zh_TW |
| dc.title | Intake of Caffeine Alters Reproduction and Development in Parthenogenetic Pea Aphid Acyrthosiphon pisum | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 李士傑;潘敏雄;洪挺軒;劉啟德 | zh_TW |
| dc.contributor.oralexamcommittee | Shyh-Jye Lee;Min-Hsiung Pan;Ting-Hsuan Hung;Chi-Te Liu | en |
| dc.subject.keyword | 豌豆蚜,人工飼料,咖啡因,生殖能力,胚胎發育,內共生菌, | zh_TW |
| dc.subject.keyword | Pea aphids,Acyrthosiphon pisum,artificial diets,caffeine,fecundity,embryogenesis,endosymbiont, | en |
| dc.relation.page | 96 | - |
| dc.identifier.doi | 10.6342/NTU202403275 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2024-08-13 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 昆蟲學系 | - |
| 顯示於系所單位： | 昆蟲學系 | |
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