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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 江伯倫 | zh_TW |
| dc.contributor.advisor | Bor-Luen Chiang | en |
| dc.contributor.author | 謝豐謄 | zh_TW |
| dc.contributor.author | Feng-Teng Hsieh | en |
| dc.date.accessioned | 2024-08-26T16:23:06Z | - |
| dc.date.available | 2024-08-27 | - |
| dc.date.copyright | 2024-08-26 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-08-07 | - |
| dc.identifier.citation | Wang, Y., Liu, J., Burrows, P. D., Wang, J. Y. (2020). B Cell development and maturation. Adv Exp Med Biol, 1254, 1-22.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/95038 | - |
| dc.description.abstract | 調節性 B 細胞 (Regulatory B cells, Bregs)是一類對免疫調節至關重要的B細胞，其定義主要依賴於它們產生的IL-10、IL-35和TGF-β等功能性分子，而不像Tregs一樣有明確的標誌分子（如Foxp3)。本研究探討了不同年齡組別小鼠脾臟和腹膜沖洗細胞以及Peyer’s patches中的Bregs族群，也對其功能性分子IL-10以及TGF-β的表現進行探討。我們將實驗分為成年組、中年組與老年組小鼠，並使用流式細胞儀分析Bregs族群，檢測脾臟中B10細胞（CD1dhiCD5+）、CD9+ B細胞、CD21hiCD23- MZ B細胞和Tim-1+ B細胞，這些Bregs族群在老化過程中呈現上升的趨勢。在腹膜沖洗細胞中，我們發現B1a細胞在中年組的比例也有增加的趨勢。這可能表示在老化過程中，腹腔中聚集了B1a細胞。而後我們分析了PP中的tolerogenic B cells (TolBC) 族群，結果顯示其比例在成年組和中年組之間保持相對一致，然而在老年組中卻顯著的上升，這些細胞的誘導Treg細胞的能力仍需要進一步研究。然後我們探討脾臟Bregs中IL-10+細胞的比例：我們發現在中年組脾臟B細胞中，IL-10+細胞的比例在脾臟Breg族群有上升的趨勢，這可能與免疫調節和老化過程有關，而將Breg純化出來進行功能性探討發現，B10、CD9+ B、B1a細胞分泌IL-10的能力在中年組相較於成年組有增加的情況，TGF-β的分泌則沒有顯著的差別，各Breg誘導T細胞死亡在年紀間沒有差別，誘導Treg分化的能力也在年紀組別間沒有顯著差別，然而，CD9+ B細胞、B1a細胞在中年組抑制T細胞增殖的能力顯著高於成年組，顯示老化可能使Breg的能力增加。整體而言，大部份Breg族群與IL-10在中年組中有增加的趨勢，並且抑制T細胞的功能也隨之上升，然而這背後的分子機制以及造成差異的原因仍有待進一步確認。 | zh_TW |
| dc.description.abstract | Regulatory B cells (Bregs) are a crucial subset of B cells involved in immune regulation, primarily defined by their production of functional molecules such as IL-10, IL-35, and TGF-β, unlike Tregs which have clear markers like Foxp3. This study investigated Breg populations in the spleen, peritoneal lavage cells, and Peyer's patches of mice from different age groups, focusing on the expression of functional molecules IL-10 and TGF-β. We divided the studying groups into young-adult, middle-aged, and old mice groups and used flow cytometry to analyze Breg populations. In the spleen, we examined B10 cells (CD1dhiCD5+), CD9+ B cells, CD21hiCD23- marginal zone (MZ) B cells, and Tim-1+ B cells, all of which showed an increasing trend with aging. In peritoneal cavity cells, we observed an increase in the proportion of B1a cells in the middle-aged and old group, indicating an accumulation of B1a cells in the peritoneum during aging. Next, we analyzed the tolerogenic B cells (TolBC) population in Peyer's patches. The results showed that its proportion remained relatively consistent between the adult and middle-aged groups but significantly increased in the old group. The ability of these cells to induce Treg cells still requires further investigation. We then examined the proportion of IL-10+ cells among splenic Bregs and found an increasing trend in the middle-aged group, which might be related to immune regulation and aging. Functional analysis of purified Bregs revealed that B10, CD9+ B, and B1a cells had an increased ability to secrete IL-10 in the middle-aged group compared to the young-adult group, while the secretion of TGF-β did not show significant differences. The ability of different Breg subsets to induce T cell death and Treg differentiation did not significantly vary with age. However, CD9+ B cells and B1a cells in the middle-aged group showed a significantly higher ability to inhibit T cell proliferation than those in the adult group, suggesting that aging might affect Breg function. Overall, most Breg populations and IL-10 production showed an increasing trend in the middle-aged group, along with an enhanced ability to inhibit T cell proliferation. However, the underlying molecular mechanisms and reasons for these changes still require further investigation. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2024-08-26T16:23:06Z No. of bitstreams: 0 | en |
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| dc.description.tableofcontents | 口試委員會審定書 I
誌謝 II 中文摘要 IV Abstract V Abbreviation VII Content VIII Contents of Figures XIII Chapter 1. Introduction 1 Part I. Background 2 1. Overview of regulatory B cells 2 1.1 Regulatory B cells (Bregs) 2 1.1.1 Splenic regulatory B cell subsets 4 1.1.1.1 B10 cell (CD19+CD1dhiCD5+) 4 1.1.1.2 CD9+ B cells (CD19+CD9+) 5 1.1.1.3 Marginal zone B cells (CD19+CD21hiCD23-) 6 1.1.1.4 Tim-1+ B cells (CD19+Tim-1+) 6 1.1.2 Peritoneal regulatory B cell subsets 7 1.1.3 Intestinal mucosa-derived regulatory B cell subset 9 2. Aging immune system 10 2.1 T cell compartment 10 2.2 B cell compartment 11 Part II. Statement of the Motives 11 Part III. Study Aims 12 Chapter 2. Materials and Methods 13 Part I. Materials 14 1. Animals 14 2. Cell culture 14 2.1 Culture medium and buffer 14 2.2 MACS cell purification 15 2.3 Mitogen used in cell culture 16 2.4 Proliferation assay 16 2.5 CFSE assay 17 3. Flow cytometry 17 3.1 Buffer 17 3.2 Antibodies used for flow cytometry 18 3.3 Device used for analysis 18 4. Enzyme-linked immunosorbent assay (ELISA) 19 Part II. Methods 20 1. Preparation of lymphocytes 20 1.1 Splenocytes 20 1.2 Peritoneal wash-out cells (PWCs) 20 1.3 B220+ B cells isolation 20 1.4 CD4+CD25- and CD4+CD25+ T cells (nTreg) isolation 21 1.5 Regulatory B cells isolation 22 1.6 Stimulation of B cells 22 2. Flow cytometry 22 2.1 Surface-marker staining 22 2.2 Intracellular staining 23 3. Suppression assay 24 3.1 Regulatory B cell activation 24 3.2 Suppression assay by [3H]-incorporation assay 24 3.3 Suppression assay by CFSE 24 4. Enzyme-linked immunosorbent assay (ELISA) 25 5. Statistical analysis 26 Chapter 3. Results 27 1. Regulatory B cells population in the spleen show an increasing trend with age 28 2. The population of B1a cells in peritoneal wash-out cells (PWCs) shows an increasing trend with age 29 3. The population of the tolerogenic B cells (TolBCs) in Peyer’s patches (PP) shows an increasing trend with age 29 4. The overlap among B10 cells (CD1dhiCD5+), CD9+ B cells, and MZ B cells (CD21hiCD23-) in young-adult, middle-aged and old mice 30 5. The proportion of IL-10+ cells in splenic Bregs shows an increasing trend with age 31 6. The proportion of FasL+ cells in Breg cells did not vary with age 33 7. In vitro protocol for activating purified Breg cells and assessing their functionality 33 8. The purity of purified cells 34 9. IL-10 production by purified Breg cells increased with age 34 10. TGF-β production by purified Breg cells did not significantly vary with age 35 11. The ability of B10 cells to induce T cell death did not significantly vary with age 36 12. CD9+ B cells and B1a cells did not significantly induce T cell death 36 13. The ability of B10 cells and CD9+ B cells to induce Tregs does not significantly change with age 37 14. B1a cells did not significantly induce Treg cells 37 15. The suppressive function of B10 cells did not significantly change with age 38 16. CD9+ B cells of the middle-aged mice had better suppressive function 38 17. B1a cells of the middle-aged mice had better suppressive function 39 Chapter 4. Discussion 40 Figures 47 References 78 | - |
| dc.language.iso | en | - |
| dc.subject | IL-10 | zh_TW |
| dc.subject | 調節性B細胞 | zh_TW |
| dc.subject | 老化 | zh_TW |
| dc.subject | 免疫調節 | zh_TW |
| dc.subject | TGF-β | zh_TW |
| dc.subject | TGF-β | en |
| dc.subject | Immune regulation | en |
| dc.subject | Aging | en |
| dc.subject | IL-10 | en |
| dc.subject | Regulatory B cells | en |
| dc.title | 探討年齡對調節性B細胞的族群與功能差異的影響 | zh_TW |
| dc.title | Study on Population and Functional Characterization of Regulatory B Cells Among Different Age Groups | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 繆希椿;劉峰誠 | zh_TW |
| dc.contributor.oralexamcommittee | Shi-Chuen Miaw;Feng-Cheng Liu | en |
| dc.subject.keyword | 調節性B細胞,IL-10,TGF-β,免疫調節,老化, | zh_TW |
| dc.subject.keyword | Regulatory B cells,IL-10,TGF-β,Immune regulation,Aging, | en |
| dc.relation.page | 88 | - |
| dc.identifier.doi | 10.6342/NTU202403576 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2024-08-08 | - |
| dc.contributor.author-college | 醫學院 | - |
| dc.contributor.author-dept | 免疫學研究所 | - |
| 顯示於系所單位： | 免疫學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-112-2.pdf 授權僅限NTU校內IP使用（校園外請利用VPN校外連線服務） | 12.05 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。