奈米電漿子影像感測器於辨識造成上呼吸道病變的飛沫傳播病毒

Abstract

自2019年末，世界開始出現造成肺部嚴重病變的特殊傳染性疫情，此嚴重上呼吸道傳染性病毒被稱為特殊的傳染肺炎（COVID-19）是由SARS-CoV-2所引發的。新冠肺炎是一種高度傳染性的病毒，近年來已傳播到全球各地。即使疫苗覆蓋率很高，感染症狀也可能極其微妙，通常類似於普通感冒，很容易被忽視，並可能危及患者的健康。此外，最近流感病毒的演變使醫療隊特別難以區分新型冠狀病毒和流感病毒。因此，開發一種具有高靈敏度、特異性、多功能性和準確檢測多種病毒能力的感測器是這項研究的動機。 新型冠狀病毒與流感病毒皆為上呼道病變的疾病，兩者病毒所產生的症狀極為相似，對於醫療團隊來說缺少一個能同時辨別新型冠狀病毒以及流感病毒的檢測裝置。本研究為了能夠快速檢測出兩者病毒，將N gene DNA作為辨識新型冠狀病毒的檢體；並且本研究將流行性感冒分為流行性感冒甲型（Influenza-A）與流行性感冒乙型（Influenza-B），將M gene DNA以及HA gene DNA分別當作流行性感冒甲型與流行性感冒乙型之檢測檢體。 本研究利用了表面電漿共振的原理配合一即時影像式SPR多重檢測系統來進行實驗。此系統不但改良了光譜量測峰值法單點量測之缺點，也將電動載台控制光譜的方法移除，以分光鏡及濾波片將單一光源分為兩段特殊波長的光，真正達到了即時檢測之效果。此研究透過將所設計之新型冠狀病毒及流行性感冒甲型、乙型病毒之生物探針修飾於晶片表面，待其與檢測目標產生專一性的鍵結後，其將在晶片表面造成介電係數變化，進而產生表面電漿影像訊號的改變，再搭配表面電漿共振影像感測器，以達到提高檢測極限和多重檢測之目的。

Since the end of 2019, the spread of the severe special infectious pneumonia pandemic began, caused by the SARS-CoV-2, leading to a global pandemic. COVID-19 is a highly contagious virus that has spread worldwide in recent years. Even with high vaccine coverage, the symptoms of infection can be very subtle, often resembling those of the common cold, making them easy to overlook and potentially endangering patients' health. Furthermore, the recent evolution of the influenza virus has made it particularly difficult for medical teams to distinguish between the novel coronavirus and the influenza virus. Therefore, developing a sensor with high sensitivity, specificity, multifunctionality, and the ability to accurately detect multiple viruses is the motivation for this research. Both the COVID-19 and the influenza virus cause upper respiratory tract diseases, and the symptoms produced by the two viruses are very similar. The lack of a detection device capable of simultaneously identifying the COVID-19 and the influenza virus presents a challenge for medical teams. In order to quickly detect both viruses, this study uses N gene DNA as the sample for identifying the COVID-19. Additionally, the study categorizes influenza into Influenza A and Influenza B, using M gene DNA and HA gene DNA as the detection samples for Influenza A and Influenza B, respectively. This research utilizes the principle of Surface Plasmon Resonance (SPR) in conjunction with a real-time imaging-based SPR multiplex detection system. This system not only improves upon the drawbacks of single-point measurement using the spectral peak method but also eliminates the use of an electric stage to control the spectrum. Instead, a beam splitter and filter divide a single light source into two specific wavelengths, achieving real-time detection. The study involves modifying the chip surface with designed biological probes for the novel coronavirus, Influenza A, and Influenza B viruses. When these probes specifically bind to their respective detection targets, they cause a change in the dielectric constant on the chip surface, resulting in a change in the surface plasmon image signal. Coupled with the surface plasmon resonance imaging sensor, this approach aims to enhance the detection limit and achieve multiplex detection.