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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳佩貞 | zh_TW |
| dc.contributor.advisor | Pei-Jen Chen | en |
| dc.contributor.author | 吳元如 | zh_TW |
| dc.contributor.author | Yuan-Ru Wu | en |
| dc.date.accessioned | 2024-08-16T16:14:09Z | - |
| dc.date.available | 2024-08-31 | - |
| dc.date.copyright | 2024-08-16 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-08-04 | - |
| dc.identifier.citation | Adeel, M., Lee, J. Y., Zain, M., Rizwan, M., Nawab, A., Ahmad, M. A., Shafiq, M., Yi, H., Jilani, G., Javed, R., Horton, R., Rui, Y., Tsang, D. C. W., & Xing, B. (2019). Cryptic footprints of rare earth elements on natural resources and living organisms. Environment International, 127, 785-800. https://doi.org/10.1016/j.envint.2019.03.022
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/94470 | - |
| dc.description.abstract | 稀土元素 (rare earth elements, REEs) 是指鑭系元素 (La－Lu)、鈧 (Scandium, Sc) 與釔 (Yttrium, Y) 共17種元素的合稱。REEs被廣泛應用在許多領域，如玻璃製造、電子科技、能源、醫療甚至農業等，近年來世界各地的使用量不斷增加，透過各種排放途徑而污染環境，最終累積在水體並可能對水中生物造成威脅，因而被視為新興污染物，目前關於REEs對水生生態的毒理和風險資料不足。本篇研究以青鱂魚 (Oryzias latipes) 作為模式生物，利用急毒性試驗探討13種REEs的 96小時半致死濃度 (96 h- Lethal concentration 50, 96 h-LC50)，以挑選毒性風險及暴露風險較高的Y (LC50 = 1.40 μM)、La (LC50 = 1.55 μM)、Gd (LC50 = 2.15 μM) 和Dy (LC50 = 2.20 μM)，再以7天的暴露實驗量測4種 REEs對青鱂魚的非致死效應，其中La (47.1 nM) 以及Y (220.3 nM) 干擾幼魚泳動行為，La (47.1 nM) 和Dy (86.6與185.2 nM) 抑制體長和體重，La具有相對較高的生物濃縮係數 (bioconcentration factors, BCFs)，La (99.4 nM)、Gd (50.1、99.4及188.8 nM) 與Dy (185.2 nM) 造成暴露魚隻的氧化壓力 (oxidative stress)。本研究利用物種敏感分布 (species sensitivity distribution, SSD) 與評估係數 (assessment factor, AF) 法計算之4種REEs的預期無影響濃度 (predicted-no effect concentration, PNEC) 顯示，PNECAF遠小於PNECSSD，且文獻指出La的環境量測濃度 (measured environmental concentration, MEC) 高 (MECave = 0.47 μg/L; MECmax = 2.41 μg/L)，因此4個REEs之中La的生態風險較高 (RQAF = 2.20–37.11; RQSSD = 0.06–0.78; RQave = 1.13–18.94)。因此，本研究評估之REEs的生態風險隨著環境濃度增加而逐漸提高，特別是La具有較高之毒性風險與暴露風險。 | zh_TW |
| dc.description.abstract | Rare earth elements (REEs), comprising the lanthanide series (La-Lu) along with scandium (Sc) and yttrium (Y), are a group of 17 elements widely used in various fields such as electronics, energy, medicine, and agriculture. The widespread applications lead to their releases into the environment through different routes, ultimately accumulating in the aquatic ecosystem and posing potential threats to the organisms. Consequently, REEs are considered as emerging contaminants. Currently, there is insufficient toxicological and risk data concerning REEs on aquatic ecosystems. In this study, we aim to screen the toxicity potency and ecological risks of REEs. The 96-hour median lethal concentration 50 (96-h LC50) of 13 REEs on larvae of medaka (Oryzias latipes) were first assessed through acute toxicity tests. Y (LC50 = 1.40 μM), lanthanum (La, LC50 = 1.55 μM), gadolinium (Gd, LC50 = 2.15 μM), and dysprosium (Dy, LC50 = 2.20 μM) were identified as 4 REEs with higher toxicity and exposure risks. Then, the 7-day sublethal exposure with medaka larvae showed impacts on swimming behaviors by La (47.1 nM) and Y (220.3 nM), decreases in body length and weight by La (99.4 nM) and Dy (86.6 and 185.2 nM), and higher bioconcentration factors (BCFs) by La. La (99.4 nM), Gd (50.1、99.4 and 188.8 nM) and Dy (185.2 nM) caused oxidative stress to medaka fish. The predicted no-effect concentrations (PNEC) derived from species sensitivity distribution (SSD) and assessment factor (AF) approaches for the 4 studied REEs showed that the PNECAF were drastically lower than the PNECSSD. Additionally, the measured environmental concentration (MEC) of La from literatures was the highest (MECave = 0.47 μg/L; MECmax = 2.41 μg/L), indicating that the ecological risk of La was the most significant (RQAF = 2.20–37.11; RQSSD = 0.06–0.78; RQave = 1.13–18.94). Therefore, our study highlights the increasing environmental risks of REEs, especially La with higher toxicity risks and exposure risks. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2024-08-16T16:14:09Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2024-08-16T16:14:09Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員審定書 i
誌謝 ii 中文摘要 iii ABSTRACT iv 目次 vi 圖次 ix 表次 xi 第一章 前言與研究動機 1 第二章 文獻回顧 3 2.1 REEs的基本性質與應用 3 2.2 REEs在環境中的污染途徑與宿命 7 2.3 REEs的水體流佈濃度 9 2.3 REEs之毒性效應 17 2.5生態風險評估方法 27 2.6模式生物 29 2.7研究目的 30 第三章 材料與方法 31 3.1研究架構及說明 31 3.2 REEs暴露溶液製備 33 3.3青鱂魚飼養與繁殖 35 3.4青鱂魚幼魚96 小時急毒性試驗 36 3.5優化REEs在VSW中之暴露條件 (用於非致死暴露試驗) 37 3.5.1暴露時間與生物對REEs溶解度的影響 37 3.5.2 溶液pH、換水頻率與魚水比對REEs溶解度的影響 37 3.6青鱂魚幼魚7日非致死毒性試驗 38 3.6.1泳動行為分析 38 3.6.2體長體重分析 39 3.6.3 REEs在魚體累積量測定 39 3.6.4 氧化壓力與神經毒性指標 40 3.7 暴露溶液中水質與REEs濃度分析 44 3.7.1 溶液pH、氧化還原電位 (Oxidation-Reduction Potential, ORP)、導電度 (Electrical conductivity, EC) 量測 44 3.7.2 REEs總濃度分析 44 3.7.3 REEs溶解態濃度分析 44 3.8 REEs之生態風險評估方法 45 3.8.1計算預期無影響濃度 (Predicted-no effect concentration, PNEC) 45 3.8.2風險商數 (Risk quotient, RQ) 46 3.9 統計分析 47 第四章 結果與討論 48 4.1急毒性試驗之REEs濃度量測與死亡率結果 48 4.2 提高REEs在VSW中溶解度的結果 (暴露條件優化) 53 4.3七日暴露實驗之非致死效應結果 58 4.3.1 Y、La、Gd與Dy在7日暴露實驗的濃度變化 58 4.3.2 Y、La、Gd與Dy在魚體的濃度及生物濃縮係數 (BCF) 58 4.3.3 泳動行為分析結果 62 4.3.4 體長體重分析結果 67 4.3.5 氧化壓力指標與乙醯膽鹼酯酶分析結果 69 4.3.6 非致死試驗測得之最低觀察到效應的濃度 (lowest observed effect concentrations, LOECs) 與無觀察到效應的濃度 (no observed effect concentrations, NOECs) 75 4.4 以SSD和AF方法計算Y、La、Gd與Dy的PNECs 77 4.4.1 PNECSSD 77 4.4.2 PNECAF 81 4.5. Y、La、Gd與Dy之RQ 83 第五章 結論與建議 86 第六章 參考文獻 88 第七章 附錄 104 | - |
| dc.language.iso | zh_TW | - |
| dc.title | 稀土元素對青鱂魚的毒性效應與生態風險評估 | zh_TW |
| dc.title | Toxicity of rare earth elements (REEs) on medaka fish and associated ecological risk assessments | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 蕭崇德;魏嘉徵;周佩欣;陳德豪 | zh_TW |
| dc.contributor.oralexamcommittee | Chung-Der Hsiao;Chia-Cheng Wei;Pei-Hsin Chou;Te-Hao Chen | en |
| dc.subject.keyword | 稀土元素,青鱂魚,新興污染物,急毒性,生物濃縮係數,生態風險評估, | zh_TW |
| dc.subject.keyword | rare earth elements,medaka (Oryzias latipes),emerging contaminants,acute toxicity,bioconcentration factors,ecological risk assessment, | en |
| dc.relation.page | 111 | - |
| dc.identifier.doi | 10.6342/NTU202403272 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2024-08-07 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 農業化學系 | - |
| dc.date.embargo-lift | 2026-08-31 | - |
| 顯示於系所單位： | 農業化學系 | |
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