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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳建甫 | zh_TW |
| dc.contributor.advisor | Chien-Fu Chen | en |
| dc.contributor.author | 陳威廷 | zh_TW |
| dc.contributor.author | Wei-Ting Chen | en |
| dc.date.accessioned | 2024-08-14T16:07:45Z | - |
| dc.date.available | 2024-08-15 | - |
| dc.date.copyright | 2024-08-13 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-08-07 | - |
| dc.identifier.citation | O. f. E. C.-o. a. Development(OECD). "Health at a Glance 2023: OECD Indicators." https://www.oecd-ilibrary.org/sites/216c1c0e-en/index.html?itemId=/content/component/216c1c0e-en (accessed 2023).
R. Nall and "What Is an Ultrasound?" https://www.health.com/ultrasound-7254193 (accessed 19/07, 2023). photographer. https://www.istockphoto.com/photo/diverse-team-of-surgeon-assistants-and-nurses-making-invasive-surgery-gm1183048938-332460823 (accessed 2019). Patrícia. "New Biomarker Assay to Aid in Systemic Sclerosis Diagnosis." https://sclerodermanews.com/news/new-biomarker-assay-aid-systemic-sclerosis-diagnosis/ (accessed 17/12, 2015). C. Dincer et al., "Disposable sensors in diagnostics, food, and environmental monitoring," Adv. Mater., vol. 31, no. 30, p. 1806739, 2019. J. Budd et al., "Lateral flow test engineering and lessons learned from COVID-19," Nature Reviews Bioengineering, vol. 1, no. 1, pp. 13-31, 2023. R. P. Feynman, "There's plenty of room at the bottom [data storage]," JMemS, vol. 1, no. 1, pp. 60-66, 1992. N. Anbusagar, K. Palanikumar, and A. Ponshanmugakumar, "Preparation and properties of nanopolymer advanced composites: A review," Polymer-based nanocomposites for energy and environmental applications, pp. 27-73, 2018. P. I. Dolez, "Nanomaterials definitions, classifications, and applications," in Nanoengineering: Elsevier, 2015, pp. 3-40. L. A. Kolahalam, I. K. Viswanath, B. S. Diwakar, B. Govindh, V. Reddy, and Y. Murthy, "Review on nanomaterials: Synthesis and applications," Mater. Today: Proc., vol. 18, pp. 2182-2190, 2019. L.-I. Batteries, "Nanomaterials for Lithium-Ion Batteries." D. Mohanta, K. Barman, S. Jasimuddin, and M. Ahmaruzzaman, "MnO doped SnO2 nanocatalysts: Activation of wide band gap semiconducting nanomaterials towards visible light induced photoelectrocatalytic water oxidation," J. Colloid Interface Sci., vol. 505, pp. 756-762, 2017. G. Chen, J. Seo, C. Yang, and P. N. Prasad, "Nanochemistry and nanomaterials for photovoltaics," Chem. Soc. Rev., vol. 42, no. 21, pp. 8304-8338, 2013. J. Li and J. Z. Zhang, "Optical properties and applications of hybrid semiconductor nanomaterials," Coord. Chem. Rev., vol. 253, no. 23-24, pp. 3015-3041, 2009. W. Liao et al., "Natural products-based nanoformulations: a new approach targeting CSCs to Cancer therapy," Int. J. Nanomed., vol. 17, p. 4163, 2022. D. Wang, S. C. Pillai, S.-H. Ho, J. Zeng, Y. Li, and D. D. Dionysiou, "Plasmonic-based nanomaterials for environmental remediation," Applied Catalysis B: Environmental, vol. 237, pp. 721-741, 2018. K. A. Willets and R. P. Van Duyne, "Localized surface plasmon resonance spectroscopy and sensing," Annu. Rev. Phys. Chem., vol. 58, pp. 267-297, 2007. F. Manea, F. Houillon, L. Pasquato, and P. Scrimin, "Nanozymes: gold-nanoparticle-based transphosphorylation catalysts," ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, vol. 43, pp. 6165-6169, 2004. L. Gao et al., "Intrinsic peroxidase-like activity of ferromagnetic nanoparticles," Nat. Nanotechnol., vol. 2, no. 9, pp. 577-583, 2007. H. Wei et al., "Nanozymes: A clear definition with fuzzy edges," Nano Today, vol. 40, p. 101269, 2021. C. Keum, C.-M. Hirschbiegel, S. Chakraborty, S. Jin, Y. Jeong, and V. M. Rotello, "Biomimetic and bioorthogonal nanozymes for biomedical applications," Nano Convergence, vol. 10, no. 1, p. 42, 2023. J. Cheong et al., "Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device," Nature biomedical engineering, vol. 4, no. 12, pp. 1159-1167, 2020. B.-H. Kang et al., "Ultrafast plasmonic nucleic acid amplification and real-time quantification for decentralized molecular diagnostics," ACS Nano, vol. 17, no. 7, pp. 6507-6518, 2023. H. Wu et al., "AIE nanozyme-based long persistent chemiluminescence and fluorescence for POCT of pathogenic bacteria," ACS Sens., vol. 8, no. 8, pp. 3205-3214, 2023. Q. H. Nguyen, D. H. Lee, P. T. Nguyen, P. G. Le, and M. I. Kim, "Foldable paper microfluidic device based on single iron site-containing hydrogel nanozyme for efficient glucose biosensing," Chem. Eng. J., vol. 454, p. 140541, 2023. C. Srisomwat, A. Yakoh, N. Chuaypen, P. Tangkijvanich, T. Vilaivan, and O. Chailapakul, "Amplification-free DNA sensor for the one-step detection of the hepatitis B virus using an automated paper-based lateral flow electrochemical device," Anal. Chem., vol. 93, no. 5, pp. 2879-2887, 2020. P. Williamson, P. Piskunen, H. Ijäs, A. Butterworth, V. Linko, and D. K. Corrigan, "Signal amplification in electrochemical DNA biosensors using target-capturing DNA origami tiles," ACS Sens., vol. 8, no. 4, pp. 1471-1480, 2023. W.-T. Chen, P.-Y. Chiu, and C.-F. Chen, "A flash signal amplification approach for ultrasensitive and rapid detection of single nucleotide polymorphisms in tuberculosis," Biosens. Bioelectron., vol. 237, p. 115514, 2023. J. Chakaya et al., "Global Tuberculosis Report 2020–Reflections on the Global TB burden, treatment and prevention efforts," Int. J. Infect. Dis., vol. 113, pp. S7-S12, 2021. K. Edwards, C. Johnstone, and C. Thompson, "A simple and rapid method for the preparation of plant genomic DNA for PCR analysis," Nucleic Acids Res., vol. 19, no. 6, pp. 1349-1349, 1991, doi: 10.1093/nar/19.6.1349. J. Libiseller-Egger, J. Phelan, S. Campino, F. Mohareb, and T. G. Clark, "Robust detection of point mutations involved in multidrug-resistant Mycobacterium tuberculosis in the presence of co-occurrent resistance markers," PLoS Comput. Biol., vol. 16, no. 12, p. e1008518, 2020. V. Singh and K. Chibale, "Strategies to combat multi-drug resistance in tuberculosis," Acc. Chem. Res., vol. 54, no. 10, pp. 2361-2376, 2021. W. Choi et al., "Multiplex SNP Genotyping Using SWITCH: Sequence-Specific Nanoparticle with Interpretative Toehold-Mediated Sequence Decoding in Hydrogel," Small, vol. 18, no. 8, p. e2105538, Feb 2022, doi: 10.1002/smll.202105538. I. C. Gray, D. A. Campbell, and N. K. Spurr, "Single nucleotide polymorphisms as tools in human genetics," Hum. Mol. Genet., vol. 9, no. 16, pp. 2403-2408, 2000. N. J. Schork, D. Fallin, and J. S. Lanchbury, "Single nucleotide polymorphisms and the future of genetic epidemiology," Clin. Genet., vol. 58, no. 4, pp. 250-264, 2000. S. Ye, S. Dhillon, X. Ke, A. R. Collins, and I. N. Day, "An efficient procedure for genotyping single nucleotide polymorphisms," Nucleic Acids Res., vol. 29, no. 17, pp. e88-e88, 2001. G. T. Walker, M. S. Fraiser, J. L. Schram, M. C. Little, J. G. Nadeau, and D. P. Malinowski, "Strand displacement amplification—an isothermal, in vitro DNA amplification technique," Nucleic Acids Res., vol. 20, no. 7, pp. 1691-1696, 1992. B. Deiman, P. van Aarle, and P. Sillekens, "Characteristics and applications of nucleic acid sequence-based amplification (NASBA)," Mol. Biotechnol., vol. 20, no. 2, pp. 163-179, 2002. K. Nagamine, T. Hase, and T. Notomi, "Accelerated reaction by loop-mediated isothermal amplification using loop primers," Mol. Cell. Probes, vol. 16, no. 3, pp. 223-229, 2002. T. Notomi, Y. Mori, N. Tomita, and H. Kanda, "Loop-mediated isothermal amplification (LAMP): principle, features, and future prospects," J. Microbiol., vol. 53, no. 1, pp. 1-5, 2015. D. Haible, S. Kober, and H. Jeske, "Rolling circle amplification revolutionizes diagnosis and genomics of geminiviruses," J. Virol. Methods, vol. 135, no. 1, pp. 9-16, 2006. M. M. Ali et al., "Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine," Chem. Soc. Rev., vol. 43, no. 10, pp. 3324-3341, 2014. M. Vincent, Y. Xu, and H. Kong, "Helicase‐dependent isothermal DNA amplification," EMBO Rep., vol. 5, no. 8, pp. 795-800, 2004. R. K. Daher, G. Stewart, M. Boissinot, and M. G. Bergeron, "Recombinase polymerase amplification for diagnostic applications," Clin. Chem., vol. 62, no. 7, pp. 947-958, 2016. J.-H. Lee et al., "Plasmonic photothermal gold bipyramid nanoreactors for ultrafast real-time bioassays," J. Am. Chem. Soc., vol. 139, no. 24, pp. 8054-8057, 2017. M. You et al., "Ultrafast photonic PCR based on photothermal nanomaterials," Trends Biotechnol., vol. 38, no. 6, pp. 637-649, 2020. J. H. Son et al., "Ultrafast photonic PCR," Light Sci. Appl., vol. 4, no. 7, pp. e280-e280, 2015. Q. Chen, Y. Zhang, H. Chen, J. Liu, and J. Liu, "Enhancing the Sensitivity of DNA and Aptamer Probes in the Dextran/PEG Aqueous Two-Phase System," Anal. Chem., vol. 93, no. 24, pp. 8577-8584, Jun 22 2021, doi: 10.1021/acs.analchem.1c01419. R. Yan et al., "Simultaneous detection of dual biomarkers using hierarchical MoS2 nanostructuring and nano-signal amplification-based electrochemical aptasensor toward accurate diagnosis of prostate cancer," Biosens. Bioelectron., vol. 197, p. 113797, 2022. W.-C. Hu, J. Pang, S. Biswas, K. Wang, C. Wang, and X.-H. Xia, "Ultrasensitive detection of bacteria using a 2D MOF nanozyme-amplified electrochemical detector," Anal. Chem., vol. 93, no. 24, pp. 8544-8552, 2021. T.-T. Tsai, C.-A. Chen, N. Yi-Ju Ho, S. Yang, and C.-F. Chen, "Fluorescent double-stranded DNA-templated copper nanoprobes for rapid diagnosis of tuberculosis," ACS Sens., vol. 4, no. 11, pp. 2885-2892, 2019. M. Singh, N. Kaur, and E. Comini, "The role of self-assembled monolayers in electronic devices," J. Mater. Chem. C, vol. 8, no. 12, pp. 3938-3955, 2020. A. Stanisławska-Sachadyn and P. Sachadyn, "MutS as a tool for mutation detection," Acta Biochim. Pol., vol. 52, no. 3, pp. 575-583, 2005. F. Cerullo et al., "Bacterial ribosome collision sensing by a MutS DNA repair ATPase paralogue," Nature, vol. 603, no. 7901, pp. 509-514, 2022. K.-W. Huang et al., "Improved performance of aminopropylsilatrane over aminopropyltriethoxysilane as a linker for nanoparticle-based plasmon resonance sensors," Sens. Actuators B Chem., vol. 163, no. 1, pp. 207-215, 2012. Y. Hao, Y. Li, L. Song, and Z. Deng, "Flash synthesis of spherical nucleic acids with record DNA density," J. Am. Chem. Soc., vol. 143, no. 8, pp. 3065-3069, 2021. H.-Y. Lin, C.-H. Huang, S.-H. Lu, I.-T. Kuo, and L.-K. Chau, "Direct detection of orchid viruses using nanorod-based fiber optic particle plasmon resonance immunosensor," Biosens. Bioelectron., vol. 51, pp. 371-378, 2014. G.-H. Chen, W.-Y. Chen, Y.-C. Yen, C.-W. Wang, H.-T. Chang, and C.-F. Chen, "Detection of mercury (II) ions using colorimetric gold nanoparticles on paper-based analytical devices," Anal. Chem., vol. 86, no. 14, pp. 6843-6849, 2014. X. Jing, X. Cao, L. Wang, T. Lan, Y. Li, and G. Xie, "DNA-AuNPs based signal amplification for highly sensitive detection of DNA methylation, methyltransferase activity and inhibitor screening," Biosens. Bioelectron., vol. 58, pp. 40-47, 2014. J. Conde, J. M. de la Fuente, and P. V. Baptista, "RNA quantification using gold nanoprobes-application to cancer diagnostics," J. Nanobiotechnology, vol. 8, no. 1, pp. 1-8, 2010. M. Larguinho and P. V. Baptista, "Gold and silver nanoparticles for clinical diagnostics—from genomics to proteomics," J. Proteomics, vol. 75, no. 10, pp. 2811-2823, 2012. Z. He et al., "Introducing DNA Nanosensor to Undergraduate Students: Rapid Non-Cross-Linking Aggregation of DNA-Functionalized Gold Nanoparticles for Colorimetric DNA Assay," J. Chem. Educ., vol. 98, no. 11, pp. 3553-3559, 2021. S.-M. Fan et al., "Detection of Hg (II) at part-per-quadrillion levels by fiber optic plasmonic absorption using DNA hairpin and DNA-gold nanoparticle conjugates," ACS Appl. Nano Mater., vol. 4, no. 10, pp. 10128-10135, 2021. B. Liu and J. Liu, "Freezing directed construction of bio/nano interfaces: reagentless conjugation, denser spherical nucleic acids, and better nanoflares," J. Am. Chem. Soc., vol. 139, no. 28, pp. 9471-9474, 2017. X. Su, R. Robelek, Y. Wu, G. Wang, and W. Knoll, "Detection of point mutation and insertion mutations in DNA using a quartz crystal microbalance and MutS, a mismatch binding protein," Anal. Chem., vol. 76, no. 2, pp. 489-494, 2004. D. Stan, C.-M. Mihailescu, R. Iosub, M. Savin, B. Ion, and R. Gavrila, "Development of an immunoassay for impedance-based detection of heart-type fatty acid binding protein," in CAS 2012 (International Semiconductor Conference), 2012, vol. 1: IEEE, pp. 157-160. A. Ahmad, P. Lee, and N. S. Jusoh, "A comparative study of thiols self-assembled monolayers on gold electrode," Middle East J. Sci. Res., vol. 24, no. 6, pp. 2152-2158, 2016. E. Briand, M. Salmain, C. Compere, and C.-M. Pradier, "Immobilization of Protein A on SAMs for the elaboration of immunosensors," Colloids Surf. B. Biointerfaces, vol. 53, no. 2, pp. 215-224, 2006. V. Humblot et al., "The antibacterial activity of Magainin I immobilized onto mixed thiols Self-Assembled Monolayers," Biomaterials, vol. 30, no. 21, pp. 3503-3512, 2009. J. Stettner et al., "A study on the formation and thermal stability of 11-MUA SAMs on Au (111)/mica and on polycrystalline gold foils," Langmuir, vol. 25, no. 3, pp. 1427-1433, 2009. P. Bhadra, M. Shajahan, E. Bhattacharya, and A. Chadha, "Studies on varying n-alkanethiol chain lengths on a gold coated surface and their effect on antibody–antigen binding efficiency," RSC Adv., vol. 5, no. 98, pp. 80480-80487, 2015. C. Gruian, E. Vanea, S. Simon, and V. Simon, "FTIR and XPS studies of protein adsorption onto functionalized bioactive glass," Biochim. Biophys. Acta Proteins Proteom., vol. 1824, no. 7, pp. 873-881, 2012. S. Kim, T. G. Kim, H. R. Byon, H.-J. Shin, C. Ban, and H. C. Choi, "Recognition of single mismatched DNA using muts-immobilized carbon nanotube field effect transistor devices," J. Phys. Chem. B, vol. 113, no. 36, pp. 12164-12168, 2009. S. Lee et al., "Synergistic enhanced rolling circle amplification based on mutS and radical polymerization for single-point mutation DNA detection," Biosens. Bioelectron., vol. 210, p. 114295, 2022. J. L. Liu, Y. C. Ma, T. Yang, R. Hu, and Y. H. Yang, "A single nucleotide polymorphism electrochemical sensor based on DNA-functionalized Cd-MOFs-74 as cascade signal amplification probes," Microchim. Acta, vol. 188, no. 8, pp. 1-10, 2021. L. T. Ngo et al., "MutS protein-based fiber optic particle plasmon resonance biosensor for detecting single nucleotide polymorphisms," Anal. Bioanal. Chem., vol. 413, no. 12, pp. 3329-3337, 2021. J. Zhang et al., "Sequence-specific detection of femtomolar DNA via a chronocoulometric DNA sensor (CDS): effects of nanoparticle-mediated amplification and nanoscale control of DNA assembly at electrodes," J. Am. Chem. Soc., vol. 128, no. 26, pp. 8575-8580, 2006. M. Mohammadniaei, A. Koyappayil, Y. Sun, J. Min, and M.-H. Lee, "Gold nanoparticle/MXene for multiple and sensitive detection of oncomiRs based on synergetic signal amplification," Biosens. Bioelectron., vol. 159, p. 112208, 2020. M. Mariconda, A. Cozzolino, P. Attingenti, F. Cozzolino, and C. Milano, "Osteoarticular tuberculosis in a developed country," J. Infect., vol. 54, no. 4, pp. 375-380, 2007. Z. Wu, W. Zhou, W. Deng, C. Xu, Y. Cai, and X. Wang, "Antibacterial and hemostatic thiol-modified chitosan-immobilized AgNPs composite sponges," ACS Appl. Mater. Interfaces, vol. 12, no. 18, pp. 20307-20320, 2020. S.-C. Wu et al., "Palladium-platinum bimetallic nanomaterials and their application in Staphylococcus aureus detection on paper-based devices," Biosens. Bioelectron., p. 114669, 2022. K. Rasool, M. Helal, A. Ali, C. E. Ren, Y. Gogotsi, and K. A. Mahmoud, "Antibacterial activity of Ti3C2T x MXene," ACS Nano, vol. 10, no. 3, pp. 3674-3684, 2016. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/93991 | - |
| dc.description.abstract | 近幾年，由於許多傳染性疾病的盛行與變異，使得對快速、靈敏且簡單的DNA診斷方法需求日益漸增。本研究目標為開發一種瞬間放大方法結合電化學偵測用於無須聚合酶鏈鎖反應的肺結核分子診斷。我們透過丁純與水溶液相互微溶的特性，使得捕獲探針DNA、單股錯配DNA與金奈米粒子溶液瞬間濃縮至微小體積，以大幅減少在溶液中所需要的反應與擴散時間。此外，由於兩段單股DNA會雜交並以超高密度結合至金奈米粒子表面，使電化學訊號顯著增加。為了降低非特異性吸附的影響與辨認錯配DNA，我們依序修飾自組裝單層與MutS蛋白質在工作電極表面。這種靈敏且專一的方法可以檢測阿托莫爾濃度程度的DNA目標物，並成功應用於檢測骨關節液中的結核病相關之單核苷酸多態性。更重要的是，由於此生物感測策略可在幾秒鐘完成，因此它在定點照護和分子診斷的應用中有巨大的潛力。 | zh_TW |
| dc.description.abstract | In recent years, the demand for rapid, sensitive, and simple methods for diagnosing deoxyribonucleic acid (DNA) has grown due to the increase in the variation of infectious diseases. This work aimed to develop a flash signal amplification method coupled with electrochemical detection for polymerase chain reaction (PCR)-free tuberculosis (TB) molecular diagnosis. We exploited the slightly miscible properties of butanol and water to instantly concentrate a capture probe DNA, a single-stranded mismatch DNA, and gold nanoparticles (AuNPs) to a small volume to reduce the diffusion and reaction time in the solution. In addition, the electrochemical signal was enhanced once two strands of DNA were hybridized and bound to the surface of the gold nanoparticle at an ultra-high density. To eliminate non-specific adsorption and identify mismatched DNA, the self-assembled monolayers (SAMs) and Muts proteins were sequentially modified on the working electrode. This sensitive and specific approach can detect as low as attomolar levels of DNA targets (18 aM) and is successfully applied to detecting tuberculosis-associated single nucleotide polymorphisms (SNPs) in synovial fluid. More importantly, as this biosensing strategy can amplify the signal in only a few seconds, it possesses a great potential for point-of-care and molecular diagnosis applications. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2024-08-14T16:07:44Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2024-08-14T16:07:45Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | Oral Examination Committee Approval i
Acknowledgements ii 摘要 iii Abstract iv Figure Contents viii Table Contents ix Chapter 1 Introduction 1 1.1. Overview of disease 1 1.1.1. Diagnosis of disease 3 1.1.2. Point-of-care test 5 1.1.3. Nanotechnology 8 1.1.4. Literature review 16 Chapter 2 Materials and Instruments 23 2.1. Materials 23 2.2. Instruments 25 Chapter 3 Development of electrochemical biosensor platform for diagnosing single nucleotide polymorphisms in tuberculosis. 26 3.1. Introduction 27 3.2. Experimental Section 30 3.2.1. AuNPs synthesis 30 3.2.2. The flash signal amplification method 31 3.2.3. Optimization of incubation time of MutS protein and SNP DNA 31 3.2.4. Determinization of sensitivity and selectivity of the electrochemical platform for SNP detection 32 3.2.5. Determine the performance of the flash signal amplification method 33 3.2.6. Detection of SNP DNA in clinical synovial fluid samples 33 3.3. Results and Discussion 34 3.3.1. Biosensing principle 34 3.3.2. Characterization of bare AuNPs and modified AuNPs 35 3.3.3. Characterization of the mix SAM-MutS/Au working electrodes 39 3.3.4. Optimization condition of incubation time of MutS protein modification and SNP DNA 41 3.3.5. Determine the selectivity of the electrochemical platform 42 3.3.6. Determine the sensitivity of the electrochemical platform 44 3.3.7. Determine the performance of the signal amplification method 45 3.3.8. Detection of SNP DNA in clinical synovial fluid samples 47 3.4. Summary 48 Chapter 4 Conclusion 49 References 50 Appendix 57 Other research results 57 Perovskite quantum dots 57 | - |
| dc.language.iso | en | - |
| dc.subject | 單核苷酸多態性 | zh_TW |
| dc.subject | 訊號放大 | zh_TW |
| dc.subject | 電化學感測 | zh_TW |
| dc.subject | 肺結核 | zh_TW |
| dc.subject | MutS 蛋白質 | zh_TW |
| dc.subject | MutS protein | en |
| dc.subject | Tuberculosis | en |
| dc.subject | Electrochemical sensing | en |
| dc.subject | Single nucleotide polymorphisms | en |
| dc.subject | Signal amplification | en |
| dc.title | 瞬間訊號放大方法用於超靈敏快速偵測肺結核中單核甘酸多態性 | zh_TW |
| dc.title | A Flash Signal Amplification Approach for Ultrasensitive and Rapid Detection of Single Nucleotide Polymorphisms in Tuberculosis | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-2 | - |
| dc.description.degree | 博士 | - |
| dc.contributor.oralexamcommittee | 蔣雅郁;余政儒;羅世強;游文岳;林子恩 | zh_TW |
| dc.contributor.oralexamcommittee | Ya-Yu Chiang;Cheng-Ju Yu;Shyh-Chyang Luo;Wen-Yueh Yu;Tzu-En Lin | en |
| dc.subject.keyword | 訊號放大,單核苷酸多態性,MutS 蛋白質,肺結核,電化學感測, | zh_TW |
| dc.subject.keyword | Signal amplification,Single nucleotide polymorphisms,MutS protein,Tuberculosis,Electrochemical sensing, | en |
| dc.relation.page | 59 | - |
| dc.identifier.doi | 10.6342/NTU202403860 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2024-08-11 | - |
| dc.contributor.author-college | 工學院 | - |
| dc.contributor.author-dept | 應用力學研究所 | - |
| 顯示於系所單位： | 應用力學研究所 | |
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