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  1. NTU Theses and Dissertations Repository
  2. 工學院
  3. 機械工程學系
請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/93943
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dc.contributor.advisor廖先順zh_TW
dc.contributor.advisorHsien-Shun Liaoen
dc.contributor.author黃凱彥zh_TW
dc.contributor.authorKai-Yan Huangen
dc.date.accessioned2024-08-09T16:35:23Z-
dc.date.available2024-08-10-
dc.date.copyright2024-08-09-
dc.date.issued2024-
dc.date.submitted2024-08-04-
dc.identifier.citation[1] G. Binnig, H. Rohrer, Ch. Gerber, and E. Weibel., "Surface Studies by Scanning Tunneling Microscopy," Phys. Rev. Lett., 49(1), pp. 57-61, Jul. 1982.
[2] G. Binnig, C. F. Quate, and Ch. Gerber., " Atomic Force Microscope," Phys. Rev. Lett., 56(9), pp. 990-993, May 1986.
[3] R. C. Barrett, C. F. Quate., "High‐speed, large‐scale imaging with the atomic force microscope," Journal of Vacuum Science & Technology B: Microelectronics and Nanometer Structures, 9(2), pp. 302-306, Mar. 1991.
[4] S. R. Manalis, S. C. Minne, C. F. Quate., "Atomic force microscopy for high speed imaging using cantilevers with an integrated actuator and sensor," Appl. Phys. Lett., 68(6), pp. 871-873, May 1996.
[5] T. Ando, N. Kodera, D. Maruyama, E. Takai, K. Saito, and A. Toda, "A High-Speed Atomic Force Microscope for Studying Biological Macromolecules in Action," Japanese Journal of Applied Physics, vol. 41, no. Part 1, No. 7B, pp. 4851-4856, 2002.
[6] A. D. L. Humphris, M. J. Miles, and J. K. Hobbs, "A mechanical microscope: High-speed atomic force microscopy," Applied physics letters, vol. 86, no. 3, pp. 034106-034106-3, 2005.
[7] T. Ando, T. Uchihashi, and T. Fukuma, "High-speed atomic force microscopy for nano-visualization of dynamic biomolecular processes," Progress in surface science, vol. 83, no. 7, pp. 337-437, 2008.
[8] Y. Suzuki, N. Sakai, A. Yoshida, Y. Uekusa, A. Yagi, Y. Imaoka, S. Ito, K. Karaki, and K. Takeyasu1, "High-speed atomic force microscopy combined with inverted optical microscopy for studying cellular events," Scientific reports, vol. 3, p. 2131, 2013.
[9] 許逸誠 (2019)。具小光點之微懸臂感測系統之設計與開發。國立臺灣大學機械工程學研究所碩士論文。
[10] 吳艾庭 (2021)。提升超小型微懸臂感應系統靈敏度之研究。國立臺灣大學機械工程學研究所碩士論文。
[11] 陳式新 (2022)。基於氦氖雷射之超小型微懸臂感測系統之設計與開發。國立臺灣大學機械工程學研究所碩士論文。
[12] 鄭守程 (2023)。提升像散式原子力顯微鏡靈敏度之研究。國立臺灣大學機械工程學研究所碩士論文。
[13] G. A. Matei, E. J. Thoreson, J. R. Pratt, D. B. Newell, N. A. Burnham , "Precision and accuracy of thermal calibration of atomic force microscopy cantilevers, " Review of Scientific Instruments, 70(8), p. 083703, Aug. 2006.
[14] E. Meyer, "Atomic force microscopy," Progress in surface science, vol. 41, no. 1, pp. 3-49, 1992.
[15] D. Rugar, H. Mamin, R. Erlandsson, J. Stern, and B. Terris. "Force microscope using a fiber-optic displacement sensor," Review of Science Instruments, vol 59, no.11, pp. 2337-2440, 1998.
[16] 黃英碩,等。像散式光學偵測系統:奈米量測新利器。科儀新知 vol. 200,頁46-65,2014。
[17] M. Tortonese, H. Yamada, R. C. Barrett and C. F. Quate, "Atomic force microscopy using a piezoresistive cantilever," TRANSDUCERS '91: 1991 International Conference on Solid-State Sensors and Actuators. Digest of Technical Papers, San Francisco, CA, USA, 1991, pp. 448-451.
[18] 買世昕 (2020)。具大量測範圍之水溶液環境力量量測系統之設計與開發。國立臺灣大學機械工程學研究所碩士論文。
[19] R. Fink-Puches, R. Hofmann-Wellenhof, J. Smolle, and H. Kerl, "Confocal laser scanning microscopy: a new optical microscopic technique for applications in pathology and dermatology," Journal of Cutaneous Pathology, vol. 22, pp. 252-259, 1995.
[20] Hans J. Tiziani, M. Wegner, Daniela Steudle, "Confocal principle for macro- and microscopic surface and defect analysis," Opt. Eng., 39(1), Jan. 2000.
[21] Ruprecht Aiko K., et al. "Confocal micro-optical distance sensor: principle and design." Optical Measurement Systems for Industrial Inspection IV, vol. 5856, 2005.
[22] Martin E. Fuerst, Ernst Csencsics, Christian Haider, and Georg Schitter. "Confocal chromatic sensor with an actively tilted lens for 3D measurement," J. Opt. Soc. Am. A, vol. 37, pp. B46-B52, 2020.
[23] Elliott AD, “Confocal Microscopy: Principles and Modern Practices,” Curr Protoc Cytom, Mar 2020.
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dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/93943-
dc.description.abstract原子力顯微鏡具奈米級解析度,並可在真空、大氣、甚至液體環境中量測樣品之表面輪廓,並已被廣泛應用於各領域。透過使用高共振頻率超小型微懸臂探針,高速原子力顯微鏡進一步提升原子力顯微鏡之成像速度,可應用於許多動態現象之量測。然而,為量測超小型之微懸臂探針,微懸臂雷射感測系統需要有更小之聚焦光點。此外,由於需高量測頻寬,亦無法使用一般的低通濾波器降低雜訊,因此較難以達到高解析度之要求。為提升微懸臂感測系統靈敏度,本研究提出一共軛焦式光路設計,透過更換不同聚焦透鏡、針孔孔徑,以及不同感測器進行靈敏度比較。其中使用氦氖雷射作為光源,並使用微懸臂探針FMAuD、聚焦透鏡49-665、50 μm針孔孔徑、位置感測器SPOT-9DMI於Gain值為30時可組合出最佳靈敏度為0.6789 mV/nm,且雜訊峰對峰值為4.2 mV,雜訊對應位移量為6.19 nm。zh_TW
dc.description.abstractAtomic force microscope(AFM) has nanoscale resolution and can measure the surface profile in vacuum, air, and even liquid environments, which has been widely used in various fields. By using an ultra-small cantilever tip with a high resonance frequency, the high-speed AFM further improves the imaging speed, which can be applied to measure many dynamic phenomena. However, in order to measure the ultra-small cantilever tip, the laser beam deflection system needs a smaller focused light spot. In addition, due to the requirement of high measurement bandwidth, common low-pass filter cannot be adopted to reduce noise, thus increasing the difficulties to achieve high resolution imaging. To improve the sensitivity of the cantilever detection system, this study proposed a confocal optical system design, and attempted to optimize the sensitivity by replacing different focusing lenses, pinhole apertures, and photodetectors. The experimental results show that the combination of a He-Ne laser, a cantilever tip FMAuD, a focusing lens 49-665, a 50 μm pinhole aperture, and a position sensitive detector SPOT-9DMI achieves a highest sensitivity of 0.6789 mV/nm with a gain value of 30. The cantilever deflection signal peak-to-peak noise is 4.2 mV, and corresponds to a displacement of 6.19 nm.en
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dc.description.tableofcontents誌謝 i
摘要 ii
Abstract iii
目次 iv
圖次 vii
表次 xiii
第一章 緒論 1
1.1研究背景 1
1.2文獻回顧 1
1.2.1 AFM之發明 1
1.2.2 HS-AFM之相關技術發展 3
1.2.3高速原子力顯微鏡微懸臂靈敏度研究 10
1.3研究目的 12
1.4內容簡介 13
第二章 原子力顯微鏡簡介 14
2.1原子力顯微鏡原理與架構 14
2.2原子力顯微鏡微懸臂探針和樣品之作用力 15
2.3微懸臂探針 16
第三章 微懸臂探針偏折量感測法 18
3.1微懸臂探針偏折量量測方法 18
3.2共軛焦顯微術 21
3.3解析度 24
3.4感測器 25
第四章 實驗架構與設計 28
4.1共軛焦式光路設計 28
4.2實驗機構 29
4.2.1雷射固定機構與光纖對位機構 30
4.2.2光路模組外殼與支架 31
4.2.3進光機構 32
4.2.4聚焦機構 33
4.2.5微懸臂機構 34
4.2.6感測器機構 36
4.3實驗儀器 38
4.3.1功率計 38
4.3.2感測器及訊號放大器 39
4.3.3壓電掃描器與控制器 41
4.3.4 Z軸步進滑台 42
4.3.5控制系統 42
4.4實驗架構與流程 43
4.4.1 訊雜比量測實驗 43
4.4.2 靈敏度量測實驗 45
第五章,實驗流程及結果 49
5.1訊雜比量測實驗 49
5.1.1訊雜比量測實驗流程 49
5.1.2訊雜比實驗結果 51
5.1.3訊雜比實驗結果分析 65
5.2靈敏度量測實驗 73
5.2.1量測靈敏度實驗流程 74
5.2.2靈敏度量測結果 75
5.2.3靈敏度結果分析 88
第六章 結論及未來展望 94
參考文獻 95
附錄 A氦氖雷射(HNL150L)規格表 97
附錄 B位置感測器SPOT-9DMI規格表 99
附錄 C PDA36A2規格表 100
附錄 D聚焦透鏡規格表(49-665) 102
附錄 E聚焦透鏡規格表(A390-A) 104
附錄 F微懸臂探針規格表(FMAuD) 105
附錄 G準直鏡規格表(F220FC-B) 106
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dc.language.isozh_TW-
dc.subject共軛焦zh_TW
dc.subject高速原子力顯微鏡zh_TW
dc.subject靈敏度zh_TW
dc.subjectSensitivityen
dc.subjectHigh-speed atomic force microscopeen
dc.subjectConfocalen
dc.title基於雷射共焦方法之微懸臂探針偵測系統zh_TW
dc.titleA Cantilever Tip Detection System based on Laser Confocal Methoden
dc.typeThesis-
dc.date.schoolyear112-2-
dc.description.degree碩士-
dc.contributor.oralexamcommittee王建凱;楊志文zh_TW
dc.contributor.oralexamcommitteeChien-Kai Wang;Chih-Wen Yangen
dc.subject.keyword高速原子力顯微鏡,共軛焦,靈敏度,zh_TW
dc.subject.keywordHigh-speed atomic force microscope,Confocal,Sensitivity,en
dc.relation.page106-
dc.identifier.doi10.6342/NTU202403286-
dc.rights.note未授權-
dc.date.accepted2024-08-07-
dc.contributor.author-college工學院-
dc.contributor.author-dept機械工程學系-
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