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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9382完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林中天(Chung-Tien Lin) | |
| dc.contributor.author | Shih-Ting Huang | en |
| dc.contributor.author | 黃詩婷 | zh_TW |
| dc.date.accessioned | 2021-05-20T20:20:02Z | - |
| dc.date.available | 2009-05-18 | |
| dc.date.available | 2021-05-20T20:20:02Z | - |
| dc.date.copyright | 2009-05-18 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-05-13 | |
| dc.identifier.citation | Abler A, Chang C. (1996). Photic injury triggers apoptosis of photoreceptor cells.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9382 | - |
| dc.description.abstract | 遺傳性視網膜退化症無論在人還是犬隻，到目前為止尚未有有效的方式可以
治癒，近年來的研究發現主要為視網膜感光細胞凋亡所造成，同時可能與微膠細 胞（microglia cells）過度活化有關。本研究利用光照曝露的方式誘導Sprague-Dawley 大鼠的視網膜病變，建立視網膜感光細胞凋亡的動物模式，用以研究遺傳性視網 膜退化症的病變，同時進行嘗試性的藥物治療，以供未來臨床治療上的另一考量。 本研究中選擇的藥物大麻二酚（cannabidiol, CBD）於之前的研究中曾被指出 具有抗氧化、抗發炎、以及神經保護的效果，並選擇已被發表於相同模式下具由 神經保護效果的minocycline 來做比較。在研究中利用視網膜電波圖以及組織病理 的型態變化來評估其具有的神經保護效果，此外進一步探討大麻二酚與微膠細胞 的活化關係，藉此確認大麻二酚達到神經保護效果的機制。結果發現以光誘導視 網膜病變的治療對照組在視網膜電波圖檢測下，比起正常大鼠有顯著的a 波以及b 波的振幅下降。組織病理檢查發現治療對照組之視網膜的外核層以及感光細胞層 厚度顯著的減少，同時外核層的細胞排列鬆散、並呈現空洞化。在免疫組織化學 染色偵測下發現，治療對照組在視網膜的外層有明顯的微膠細胞活化。研究發現 大麻二酚在視網膜電波圖以及組織病理的評估下，皆能減緩光誘導之視網膜病 變，並且抑制微膠細胞的活化，比起治療對照組有顯著差異。因此認為大麻二酚 具有神經保護效果。但在臨床上對於視力的實際保護效力未來仍需要更進一步的 評估。 | zh_TW |
| dc.description.abstract | Inherited retinopathy, progressive retinal atrophy（PRA）, is a group of common
inherited retinopathies in dogs. There is no treatment for inherited retinopathy to date. Loss of vision cells by apoptosis is a key feature of PRA. Microglia cells have been believed to play an important role in neurodegenerative diseases. Light-induced retinal degeneration has been a good experimental model to study the pathophysiology and therapeutic modalities in photoreceptor-led retinal degeneration. The purpose of this study was to evaluate the neuroprotective effect of cannabidiol（CBD）to investigate whether CBD can ameliorate light-induced retinal degeneration. Sprague-Dawley rats received an intraperitoneal injection of CBD, minocycline or vehicle 12 hours before exposure to white light. Morphologic, immunohistochemical, and scotopic electroretinograms were performed to evaluate the effect of CBD and the possible involvement of microglial cells. After exposure to light scotopic electroretinography showed that both the amplitude of a- and b-wave significantly reduced of the vehicle-treated group. Retinal histology revealed that the outer nuclear layer and outer segment of the retina were significantly decreased in vehicle-treated group, and OX42-positive microglia occurred in the ONL and subretinal space. The CBD-treated group showed markedly better preservation of retina electrophysiologically and morhologically after light damage, and significantly reduced the number of OX42-positive microglia than the vehicle-treated group. CBD may process neuroprotective effect against photoreceptor degeneration following light exposure based on this study, and possibly through the inhibition of retinal microglial activation. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-20T20:20:02Z (GMT). No. of bitstreams: 1 ntu-98-R95629034-1.pdf: 2719471 bytes, checksum: 2a58198eebc38a1f39da05afa0772377 (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 中文摘要………………………………………………………………….……………1
英文摘要………………………………………………………………….……………2 第一章 緒言 ........................................................................................................... 3 第一節 犬隻遺傳性視網膜疾病之更新 ....................................................... 3 第二節 光照與眼部病變的關連性 ............................................................... 3 第三節 實驗目的 ........................................................................................... 4 第二章 文獻探討 ....................................................................................................... 5 第一節 視網膜退化症（Retina atrophy）以及目前治療策略之介紹 ........... 5 第1-1 節 視網膜退化症（Retina atrophy） ............................................ 5 第1-2 節 視網膜退化症分類 .................................................................... 7 第1-3 節 視網膜退化症之臨床探討 ...................................................... 10 第1-4 節 治療策略 .................................................................................. 13 第二節 光誘導視網膜病變之動物模式 ..................................................... 13 第2-1 節 簡介 .......................................................................................... 13 第2-2 節 光誘導視網膜病變生理病理機制之探討 .............................. 14 第2-3 節 光誘導視網膜病變之評估 ...................................................... 18 第2-4 節 治療策略 .................................................................................. 24 第三節 大麻二酚（Cannabidiol）的簡介與應用 ...................................... 27 第3-1 節 大麻生物鹼及其臨床應用潛力 .............................................. 27 第3-2 節 大麻二酚的作用機制簡介 ...................................................... 29 第四節 Minocycline 的簡介與臨床應用 .................................................... 32 第4-1 節 Minocycline 的簡介及臨床潛力 ............................................ 32 第4-2 節 Minocycline 的神經保護機制簡介 ........................................ 33 第三章 實驗材料與方法 ..................................................................................... 36 II 第一節 實驗動物 ......................................................................................... 36 第二節 光誘導視網膜病變之大鼠模式 ..................................................... 36 第三節 實驗材料與分組 ............................................................................. 36 第四節 藥物的劑量與投予 ......................................................................... 37 第五節 視網膜電波圖之紀錄與分析 ......................................................... 38 第六節 組織病理學切片評估 ..................................................................... 39 第七節 微膠細胞（Microglia cell）免疫組織化學染色評估 ................... 39 第四章 實驗結果 ................................................................................................. 42 第一節 視網膜電波圖之分析結果 ............................................................. 42 第1-1 節 視網膜電波圖a 波之分析結果 .............................................. 42 第1-2 節 視網膜電波圖b 波之分析結果 .............................................. 47 第二節 組織病理學切片評估 ..................................................................... 50 第2-1 節 視網膜各層厚度之分析比較 .................................................. 50 第2-2 節 視網膜型態之檢查結果 .......................................................... 60 第三節 微膠細胞（Microglia cell）免疫組織化學染色之分析 ............... 62 第五章 討論 ......................................................................................................... 67 第一節 光誘導視網膜退化大鼠模式之建立 ................................................. 67 第二節 神經保護藥物的選擇 ......................................................................... 68 第三節 大麻二酚的藥效評估………………………………………………...70 第六章 結論……………………………………………….……………………..81 第七章 參考文獻 ................................................................................................. 82 | |
| dc.language.iso | zh-TW | |
| dc.title | 大麻二酚在光誘導視網膜退化病變模式下的神經保護效果 | zh_TW |
| dc.title | Neuroprotective Effect of Cannabidiol in Experimental Light-induced Retinal Degeneration | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 詹東榮(Tong-Rong Jan) | |
| dc.contributor.oralexamcommittee | 劉振軒,楊繼,何素鵬 | |
| dc.subject.keyword | 大麻二酚,視網膜退化,光傷害,神經保護, | zh_TW |
| dc.subject.keyword | cannabidiol,CBD,retinal degeneration,light damage,neuroprotection, | en |
| dc.relation.page | 87 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2009-05-14 | |
| dc.contributor.author-college | 獸醫專業學院 | zh_TW |
| dc.contributor.author-dept | 臨床動物醫學研究所 | zh_TW |
| 顯示於系所單位： | 臨床動物醫學研究所 | |
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