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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 薛雁冰 | zh_TW |
| dc.contributor.advisor | Yen-Ping Hsueh | en |
| dc.contributor.author | 林子翔 | zh_TW |
| dc.contributor.author | Tzu-Hsiang Lin | en |
| dc.date.accessioned | 2024-07-17T16:28:11Z | - |
| dc.date.available | 2024-07-18 | - |
| dc.date.copyright | 2024-07-17 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-07-10 | - |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/93110 | - |
| dc.description.abstract | 獵食行為可以引起獵物在行為上的改變，然而獵物面對獵食壓力的分子和神經機制機理仍不清楚。在本研究中，我們探討了模式線蟲Caenorhabditis elegans（秀麗隱桿線蟲）對線蟲捕捉菌Arthrobotrys oligospora（寡孢子節叢孢菌）獵食時的反應。我們發現A. oligospora的陷阱捕捉會誘導獵物線蟲C. elegans進入行為靜止狀態，顯現咽部泵動和身體運動的快速停止。對被A. oligospora捕獲的C. elegans進行鈣離子影像紀錄顯示靜止狀態的發生是由促進睡眠的神經元 ALA 和 RIS 的活化所調控。利用神經發育的突變株分析證實了 ALA 神經元對於抑制咽部泵動至關重要，而 ALA 和 RIS 神經元皆參與於停止運動。此外，被A. oligospora陷阱捕捉的C. elegans的轉錄組分析揭示了防禦和免疫反應基因表現的上升，包括抗菌肽、與代謝異源物質有關的基因以及p38 MAPK途徑。最後，我們論證了A. oligospora捕獲時需要機械感覺神經元來抑制咽部泵動和調控轉錄組變化。這些發現表明，線蟲捕捉菌陷阱造成的物理刺激誘發了機械感覺依賴性的機械壓力，導致了C. elegans壓力誘導的行為靜止和防禦免疫基因的上升。我們推測陷阱誘導的行為靜止可能是一種由固著性獵食者用來在演化軍備競賽中取得生存優勢的獵食策略。 | zh_TW |
| dc.description.abstract | Predation can induce behavioral changes in prey, yet the molecular and neuronal mechanisms underlying prey responses to predation stress remain poorly understood. Here, we investigated the response of the nematode Caenorhabditis elegans to predation by the nematode-trapping fungus, Arthrobotrys oligospora. We found that A. oligospora predation induced a quiescent state in C. elegans, leading to the rapid cessation of pharyngeal pumping and body movement. Calcium imaging of A. oligospora-trapped C. elegans revealed that the quiescent state was regulated by the activation of the sleep-promoting neurons, ALA and RIS. Genetic analyses demonstrated that ALA neurons were essential for pharyngeal pumping inhibition, whereas both ALA and RIS neurons contributed to movement cessation. Furthermore, transcriptomic analysis in A. oligospora-trapped C. elegans revealed the upregulation of defense and immune response genes, including antimicrobial peptides, xenobiotic metabolism genes, and the p38 MAPK pathway. Lastly, we demonstrated that mechanosensory neurons were required for pumping inhibition and transcriptomic regulation upon A. oligospora trapping. These findings suggest that physical constraints imposed by fungal traps trigger mechanosensory-dependent mechanical stress, resulting in stress-induced quiescence and the upregulation of defense genes in C. elegans. We posit that trap-induced quiescence is a predation strategy used by sessile predators to prevail in the evolutionary arms race. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2024-07-17T16:28:11Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2024-07-17T16:28:11Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
謝辭 ii 中文摘要 iii Abstract iv 目次 v 圖次 vii 表次 viii Chapter 1. Introduction 1 Chapter 2. Results 5 2.1 A. oligospora predation induces behavioral quiescence in C. elegans 5 2.2 The sleep-promoting neurons ALA and RIS are activated in response to A. oligospora trapping 6 2.3 The sleep-promoting neurons regulate pharyngeal pumping and movement inhibitions 8 2.4 Mechanosensory neurons regulate trapping-induced pharyngeal pumping 9 2.5 A. oligospora trapping induces up-regulation of defense and immune response genes 11 2.6 Trapping-induced transcriptomic regulations are highly correlated with mechanical stress 13 2.7 Reactive oxygen species and epidermal growth factor receptor signaling pathways for trapping-induced sleep 14 Chapter 3. Discussion 17 Chapter 4. Materials and Methods 35 4.1 Key resources table 35 4.2 Experimental model and study participant details 39 4.3 Arthrobotrys oligospora trap induction 39 4.4 Quantifying pharyngeal pumping 40 4.5 Tracking head movements 40 4.6 Head-drop avoidance assay 41 4.7 Neuronal calcium imaging 41 4.8 WormGlu physical restriction 42 4.9 RNA-seq transcriptomic analysis 42 4.10 Cross-correlation analysis 43 4.11 CRISPR-Cas9 knockout 43 4.12 ROS in vivo imaging 44 4.13 Antioxidant NAC treatment 44 4.14 Quantification and Statistical Analysis 45 Acknowledgments 45 References 46 | - |
| dc.language.iso | en | - |
| dc.subject | 寡孢子節叢孢菌 | zh_TW |
| dc.subject | 機械感覺 | zh_TW |
| dc.subject | 獵食者—獵物交互作用 | zh_TW |
| dc.subject | RNA定序 | zh_TW |
| dc.subject | 線蟲捕捉菌 | zh_TW |
| dc.subject | 獵食者誘導靜止 | zh_TW |
| dc.subject | 秀麗隱桿線蟲 | zh_TW |
| dc.subject | 睡眠促進神經元 | zh_TW |
| dc.subject | RNA-seq | en |
| dc.subject | Nematode-trapping fungi | en |
| dc.subject | mechanosensation | en |
| dc.subject | sleep-promoting neuron | en |
| dc.subject | predator-induced quiescence | en |
| dc.subject | C. elegans | en |
| dc.subject | A. oligospora | en |
| dc.subject | predator-prey interactions | en |
| dc.title | 線蟲捕捉菌的獵食行為誘導秀麗隱桿線蟲的機械感覺依賴行為靜止 | zh_TW |
| dc.title | Predation by nematode-trapping fungus triggers mechanosensory-dependent quiescence in C. elegans | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 吳玉威;潘俊良 | zh_TW |
| dc.contributor.oralexamcommittee | Yu-Wei Wu;Chun-Liang Pan | en |
| dc.subject.keyword | 線蟲捕捉菌,寡孢子節叢孢菌,秀麗隱桿線蟲,獵食者誘導靜止,睡眠促進神經元,機械感覺,獵食者—獵物交互作用,RNA定序, | zh_TW |
| dc.subject.keyword | Nematode-trapping fungi,A. oligospora,C. elegans,predator-induced quiescence,sleep-promoting neuron,mechanosensation,predator-prey interactions,RNA-seq, | en |
| dc.relation.page | 49 | - |
| dc.identifier.doi | 10.6342/NTU202400924 | - |
| dc.rights.note | 同意授權(全球公開) | - |
| dc.date.accepted | 2024-07-11 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 基因體與系統生物學學位學程 | - |
| 顯示於系所單位： | 基因體與系統生物學學位學程 | |
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