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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 徐善慧 | zh_TW |
dc.contributor.advisor | Shan-Hui Hsu | en |
dc.contributor.author | 黃亮鈞 | zh_TW |
dc.contributor.author | Liang-Jyun Huang | en |
dc.date.accessioned | 2024-07-08T16:13:19Z | - |
dc.date.available | 2024-07-09 | - |
dc.date.copyright | 2024-07-08 | - |
dc.date.issued | 2024 | - |
dc.date.submitted | 2024-07-04 | - |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/92951 | - |
dc.description.abstract | 具備鄰苯二酚基團的水凝膠因具備強大的黏附性、內聚能力、生物相容性和止血能力,而受到生物醫學領域的關注。鄰苯二酚官能化殼聚醣有望於製備自組裝水凝膠。然而,這類水凝膠仍然存在凝膠化效率低落和膠體解構不穩定的問題。在本研究中,合成了高取代度(~28%)的鄰苯二酚殼聚醣(Chitosan-Catechol),並將CC與同樣含有鄰苯二酚官能基的單寧酸結合,形成具備自癒合能力的CC-TA水凝膠。富含鄰苯二酚的CC-TA複合水凝膠具備快速的凝膠效率和更佳的機械性質(剪切模量約110 Pa)。原位相干小角度X射線散射(SAXS)結合流變學分析得出了CC-TA水凝膠內會形成奈米尺度團簇(~20 nm)的形態特徵。這些團簇在大幅度的動態剪切壓力下會被破壞,而此現象與CC-TA水凝膠的應變依賴性相互對應。此外,該複合水凝膠具有滲透響應性和優秀的黏附性能。與此同時,透過簡單的凍融程序所製備的CC-TA複合冷凍凝膠展現了獨特的大孔結構(約200 µm)、高溶脹率(約7000%)和良好的壓縮模量(約8 kPa)。這種類海綿結構的冷凍凝膠具備製成止血棉花的潛力,並且展現了止血能力。CC-TA衍生複合物,無論是水凝膠還是冷凍凝膠形式都具有自由基清除能力、抗菌能力和細胞相容性,這些性質幫助其在進一步的生物應用中提供潛力。 | zh_TW |
dc.description.abstract | Hydrogels containing catechol group have received attention in the biomedical field due to their robust adhesive/cohesive capabilities, biocompatibility, and hemostatic abilities. Catechol-functionalized chitosan holds promise for preparing self-assembly hydrogels. However, issues of inefficient gelation and instability still persist in these hydrogels. In the current study, we synthesized chitosan catechol (CC) of high catechol substitution (~28%) and combined CC with tannic acid (TA, which also contains catechol) to form self-healing CC-TA hydrogels. The catechol-enriched CC-TA composite hydrogels showed rapid gelation and mechanical reinforcement (shear modulus ~110 Pa). In situ coherent small-angle X-ray scattering (SAXS) coupled with rheometry revealed a morphological feature of nanoscale clusters (~20 nm) within CC-TA hydrogel. The clusters underwent dynamic destruction under large-amplitude oscillatory shear, corresponding with the strain-dependent and self-healing behavior of the CC-TA hydrogel. The composite hydrogel had osmotic-responsive and notable adhesive properties. Meanwhile, CC-TA composite cryogel prepared simply through freeze-thawing procedures exhibited distinctive macroporous structure (~200 µm), high water swelling ratio (~7000%), and favorable compressive modulus (~8 kPa). The sponge-like cryogel was fabricated into swabs, demonstrating hemostatic capacity. The CC-TA composites, in both hydrogel and cryogel forms, possessed ROS scavenging ability, antimicrobial activity, and cell compatibility with potentials in biological applications. | en |
dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2024-07-08T16:13:19Z No. of bitstreams: 0 | en |
dc.description.provenance | Made available in DSpace on 2024-07-08T16:13:19Z (GMT). No. of bitstreams: 0 | en |
dc.description.tableofcontents | 審定書 I
誌謝 II 中文摘要 III 英文摘要 IV 目次 V 圖次 VII 表次 VIII 第一章 文獻回顧 1 1.1.殼聚醣的應用限制與化學改質 1 1.2.殼聚醣水凝膠及冷凍凝膠之成膠原理與特性 1 1.3.單寧酸的基本性質介紹及應用 2 1.4.研究目的 3 1.5 研究架構 3 第二章 研究方法 5 2.1. 鄰苯二酚-殼聚醣之合成與鑑定 5 2.1.1. 鄰苯二酚-殼聚醣之合成 5 2.1.2. 紫外-可見光光譜(UV-Vis)之取代度計算 6 2.2. CC和CC-TA水凝膠製備及定性成分分析 6 2.2.1. 水凝膠之製備 6 2.2.2. 傅立葉轉換紅外光譜(FT-IR)之水膠成分分析 6 2.3. CC和CC-TA 水凝膠的流變學分析 7 2.4. 透過小角度 X 光散射對CC和CC-TA 水凝膠進行微結構分析 7 2.5. CC-TA 水凝膠的性能測試 7 2.5.1. 注射性測試 8 2.5.2. 黏附性測試 8 2.5.3. CC-TA水凝膠巨觀自癒合測試 9 2.6. CC-TA冷凍凝膠的製備及測試 2.6.1. CC-TA凍膠製備流程及觀察 9 2.6.2. 黏附性測試 9 2.6.3. 凍膠的溶脹率分析 10 2.6.4. 凍膠的動態力學分析 10 2.7. CC-TA 水凝膠和凍膠的抗氧化性 10 2.8. CC-TA 水凝膠和凍膠的抗菌能力 11 2.9. CC-TA 水凝膠和冷凍凝膠的體外生物相容性 12 2.10. 凝血實驗 12 2.11. 統計學分析 14 第三章 實驗結果 15 3.1. 鄰苯二酚殼聚醣的合成和表徵 15 3.2. CC-TA水凝膠和凍凝膠的製備和定性分析 16 3.3. CC與CC-TA水凝膠的熱重分析 17 3.4. 不同鄰苯二酚取代度的CC和CC-TA水凝膠的流變性質 18 3.5. CC-TA水凝膠的相干小角度X光微結構分析 22 3.6. CC-TA水凝膠的注射性、黏附性和自癒合性質 24 3.7. CC-TA冷凍凝膠的製備和表徵 26 3.8. CC-TA水膠和冷凍凝膠的抗氧化活性 29 3.9. CC和CC-TA水凝膠和冷凍凝膠的抗菌活性 30 3.10. CC-TA水膠和冷凍凝膠的體外細胞相容性 31 3.11. CC-TA水凝膠和冷凍凝膠的體外凝血實驗 32 第四章 討論 35 結論 41 參考文獻 42 | - |
dc.language.iso | zh_TW | - |
dc.title | 鄰苯二酚殼聚醣-單寧酸複合水凝膠和冷凍凝膠 的抗菌和止血性質 | zh_TW |
dc.title | Chitosan Catechol-Tannic Acid Composite Hydrogel and Cryogel with Antimicrobial and Hemostatic Properties | en |
dc.type | Thesis | - |
dc.date.schoolyear | 112-2 | - |
dc.description.degree | 碩士 | - |
dc.contributor.oralexamcommittee | 張書瑋;周佳靚 | zh_TW |
dc.contributor.oralexamcommittee | Shu-Wei Chang;Chia-Ching Chou | en |
dc.subject.keyword | 鄰苯二酚殼聚醣,單寧酸,自組裝水凝膠,自癒合水凝膠,黏附性, | zh_TW |
dc.subject.keyword | Chitosan catechol,Tannic acid,Self-assembly hydrogel,Self-healing,Bioadhesive, | en |
dc.relation.page | 47 | - |
dc.identifier.doi | 10.6342/NTU202401200 | - |
dc.rights.note | 同意授權(限校園內公開) | - |
dc.date.accepted | 2024-07-04 | - |
dc.contributor.author-college | 工學院 | - |
dc.contributor.author-dept | 高分子科學與工程學研究所 | - |
顯示於系所單位: | 高分子科學與工程學研究所 |
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