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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 何佳安 | zh_TW |
| dc.contributor.advisor | Ja-an Annie Ho | en |
| dc.contributor.author | 孫婉慈 | zh_TW |
| dc.contributor.author | Wan-Tzu Sun | en |
| dc.date.accessioned | 2024-03-21T16:34:02Z | - |
| dc.date.available | 2024-10-04 | - |
| dc.date.copyright | 2024-03-21 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-02-05 | - |
| dc.identifier.citation | 1. Bardill, J.R., et al., Topical gel-based biomaterials for the treatment of diabetic foot ulcers. Acta biomaterialia, 2022. 138: p. 73-91.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/92312 | - |
| dc.description.abstract | 糖尿病足潰瘍（Diabetes foot ulcers，DFU）是一種嚴重的糖尿病併發症。在糖尿病患者中，高血糖會導致過量的Reactive oxygen species（ROS）產生，加劇傷口的氧化壓力和發炎反應，損害皮膚的屏障功能，進而導致傷口修復能力受損。糖尿病傷口患者是截肢的高風險族群，疼痛等負面影響，嚴重降低患者的生活品質，因此迫切需要一種新穎且有效的治療策略。
本研究旨於設計一種由海藻酸鈉、明膠以及透明質酸配置成的可注射水凝膠以包覆Cyanidin-3-glucoside（C3G）。在糖尿病傷口處深層滲透、持續釋放，以改善傷口環境並促進癒合。研究中透過DCFDA螢光探針方法證明，C3G能降低H2O2處理後成纖維細胞和角質形成細胞內ROS含量。透過劃痕實驗證實，C3G能回復氧化壓力所抑制的細胞遷移能力，提升患部的癒合。在Western blot結果發現，C3G能顯著性提升成纖維細胞和角質形成細胞的抗氧化蛋白HO-1表達，提升細胞抗氧化能力。此外，本研究發現，C3G水凝膠具有促進傷口治療的功能性，包括可注射性、高自適性、高吸水能力、低細胞毒性等特性。並且C3G 水凝膠亦可清除細胞中 ROS，改善傷口高氧化壓力，提昇內皮細胞遷移速率，從而促進上皮形成和傷口縮合的效能。總結來說，C3G水凝膠藥物釋放系統能夠有效恢復皮膚再生能力，促進傷口癒合，對於治療糖尿病足潰瘍是具有前景的替代方法。 | zh_TW |
| dc.description.abstract | Diabetes foot ulcer(DFU) is a severe diabetes complication, and one of the most common type of chronic wound[1]. In diabetic patients, hyperglycemia causes excessive production of ROS, and accelerates oxidative stress and inflammation, compromising the barrier function of skin that leads to impaired wound repair[2, 3]. Considering the high risk of limb amputation and negative impact on patients’ quality of life, a novel yet effective therapeutic strategy for diabetic wounds is in urgent need. We herein designed and fabricated an injectable, biocompatible sodium gelatin/alginate/hyaluronic acid (G/SA/HA)-base hydrogel that could sustainedly release the therapeutic payload, cyanidin-3-glucoside(C3G). Our primary results reveal that C3G significantly reduced ROS levels in H2O2-treated L929 fibroblast and HaCaT keratinocytes, which were measured by the 2’,7’-dichlorofluorescin diacetate(DCFDA) assay. Furthermore, C3G proved up-regulates heme oxygenase-1(HO-1) protein expressions, protecting L929 and HaCaT cells damage against oxidative damage. Additionally, C3G was found to enhance the proliferation and migration of L929 and HaCaT cells, resulting in an improved epithelialization and wound contraction. In summary, our SA/G/HA hydrogel-based drug delivery system demonstrated multi-functionality, including injectability, self-adapting behavior and low cytotoxicity, suggesting that it is a promising alterative to achieve complete dermal regeneration in patients with DFUs. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2024-03-21T16:34:02Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2024-03-21T16:34:02Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 謝辭 I
摘要 II Abstract III 目次 IV 1 第一章 緒論 1 1.1 研究背景與動機 1 1.2 研究目的 2 1.3 實驗設計圖 3 2 第二章 文獻回顧 4 2.1 糖尿病現況概述 4 2.2 糖尿病傷口 6 2.2.1 糖尿病傷口介紹 6 2.3 活性氧ROS對傷口癒合的影響 8 2.4 纖維母細胞對傷口的影響 9 2.5 角質形成細胞對傷口的影響 9 2.6 糖尿病傷口對纖維母細胞及角質形成細胞的影響 10 2.7 傷口治療診斷分類 11 2.8 臨床治療糖尿病傷口方法 12 2.8.1 傷口清創（Debridement） 12 2.8.2 負壓治療（Negative Pressure Therapy and Off-Loading） 13 2.8.3 高壓氧治療（Hyperbaric Oxygen Therapy） 13 2.8.4 生長因子的療法（Growth Factor-Based Therapies） 13 2.8.5 敷料和水凝膠（Dressings and Hydrogels） 14 2.9 明膠（Gelatin，G） 15 2.10 海藻酸鈉（Sodium Alginate，SA） 15 2.11 透明質酸（Hyaluronic Acid，HA） 16 2.12 矢車菊素-3-葡萄糖苷（Cyanidin-3-glucoside，C3G）介紹 17 3 第三章 材料與方法 18 3.1 實驗儀器 18 3.2 實驗試劑藥品及耗材 20 3.3 實驗細胞株 27 3.4 實驗方法 28 3.4.1 明膠/海藻酸鈉/透明質酸複合水凝膠之製備 28 3.4.2 明膠/海藻酸鈉/透明質酸複合水凝膠之可注射能力測試 29 3.4.3 明膠/海藻酸鈉/透明質酸複合水凝膠之溶脹能力測試 29 3.4.4 明膠/海藻酸鈉/透明質酸複合水凝膠之自適性測試 30 3.4.5 細胞培養 31 3.4.6 細胞存活率測試（MTT assay） 32 3.4.7 細胞內ROS含量測試（Cellular ROS Detection） 35 3.4.8 花青素水凝膠之抗氧化能力測試 36 3.4.9 西方墨點法（Western Blotting） 37 3.4.10 傷口癒合實驗（Wound healing assay） 43 4 第四章 實驗結果與討論 44 4.1 不同比例之明膠/海藻酸鈉/透明質酸水凝膠流變性質 44 4.2 明膠/海藻酸鈉/透明質酸水凝膠具可注射能力 50 4.3 明膠/海藻酸鈉/透明質酸複合水凝膠之溶脹能力 54 4.4 明膠/海藻酸鈉/透明質酸複合水凝膠之自適性 57 4.5 明膠/海藻酸鈉/透明質酸複合水凝膠具有良好生物相容性 62 4.6 Cyanidin-3-glucoside 具有良好之生物相容性 64 4.7 H2O2誘導氧化壓力對細胞活性的影響 66 4.8 Cyanidin-3-glucoside降低細胞內ROS含量 68 4.9 Cyanidin-3-glucoside水凝膠降低細胞內ROS含量 75 4.10 Cyanidin-3-glucoside提升抗氧化蛋白HO-1表達 83 4.11 Cyanidin-3-glucoside促進表皮細胞爬行 86 5 第五章 結論與未來展望 90 參考文獻 92 | - |
| dc.language.iso | zh_TW | - |
| dc.title | 開發可注射式水凝膠系統：控制釋放花青素以促進糖尿病患者傷口癒合 | zh_TW |
| dc.title | Hydrogel-based Drug Delivery System Targeting Oxidative Stress for Diabetic Wound Management | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 吳立真;徐士蘭;廖明淵 | zh_TW |
| dc.contributor.oralexamcommittee | Li-chen Wu;Shih-Lan Hsu;Ming-Yuan Liao | en |
| dc.subject.keyword | 糖尿病足潰瘍,ROS,水凝膠,傷口癒合,纖維母細胞,角質形成細胞,Cyanidin-3-glucoside, | zh_TW |
| dc.subject.keyword | Diabetic wounds,ROS,Cyanidin-3-glucoside,hydrogel,Diabetes foot ulcer, | en |
| dc.relation.page | 99 | - |
| dc.identifier.doi | 10.6342/NTU202400450 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2024-02-06 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 生化科技學系 | - |
| 顯示於系所單位： | 生化科技學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-112-2.pdf 未授權公開取用 | 5.33 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。