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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳凱儀 | zh_TW |
| dc.contributor.advisor | Kai-Yi Chen | en |
| dc.contributor.author | 蘇育賢 | zh_TW |
| dc.contributor.author | Yu-Hsien Su | en |
| dc.date.accessioned | 2024-03-04T16:20:50Z | - |
| dc.date.available | 2024-03-05 | - |
| dc.date.copyright | 2024-03-04 | - |
| dc.date.issued | 2024 | - |
| dc.date.submitted | 2024-02-04 | - |
| dc.identifier.citation | 方昱富. (2018). 高溫下番茄花粉數量及花粉活性之數量性狀基因座定位以及轉錄體分析. 臺灣大學農藝學研究所碩士學位論文.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/92063 | - |
| dc.description.abstract | 隨著氣候變遷加劇，未來的溫度將會越來越高，而番茄的產量也會隨之受到 影響。目前常用的番茄耐熱性指標包括花粉活性、花粉數量和著果率。花粉活性 與花粉數量用於評估雄配子，而著果率則用於評估雌配子。種子數目或可作為組 合雌雄配子耐熱特性的新指標。本研究使用耐熱親本 Siberia 和不耐熱親本 Tstar 雜交所生產的 176 個重組自交系進行試驗，分別調查在常溫與長期和緩高溫之 下果實的種子數量。外表型資料顯示，在長期和緩高溫之下的種子數目會較在常 溫下的種子數目來得低。同時使用 RAD capture 技術取得遺傳定位族群個體的分 子標記基因型，用以建立連鎖圖譜。總共獲得 12,525 個在兩親本上有差異的單 核苷酸多型性標誌。經過篩選後，最終使用 1,336 個標誌進行連鎖圖譜建立，得 到總長度 1955.7 cM、16 個連鎖群的遺傳圖譜。將外表型資料與基因型資料相結 合，進行種子數與果實心室數目的數量性狀基因座定位分析。試驗結果顯示，僅 在 2023 年的春作試驗發現一個影響種子數目變異的數量性狀基因座，與位於第 二對染色體 45.2Mb 的分子標記緊密連鎖，此數量性狀基因座可解釋種子數目全 部外表型變異的 20.03%。而相同的分子標記亦是與果實心室數目數量性狀基因 座最為緊密連鎖的分子標記。綜合以上結果，種子數目在此遺傳定位族群中並非 一個合適評估番茄耐熱特性的指標。 | zh_TW |
| dc.description.abstract | As climate change intensifies, tomato production will be impacted by rising temperatures. Pollen viability, pollen number, and fruit set rate are three commonly used indicators to evaluate heat tolerance in tomatoes. Pollen viability and pollen number are used to assess male gametes, while fruit set rate is for female gametes. Seed number might be a new characteristic as a heat-tolerant indicator for combining male and female gametes. This study used 176 recombinant inbred lines produced by crossing the heat- tolerant parent Siberia and the heat-sensitive parent Tstar to conduct experiments investigating the number of seeds in fruits under ordinary temperatures and long-term moderate high temperature. Phenotypic data showed fewer seed number under long- term mild high temperature than those under ordinary temperatures. At the same time, the RAD-capture technique was used to acquire genotypes of molecular markers of individuals in the genetic mapping population, allowing for the construction of linkage maps. A total of 12,525 SNP markers that differed between the two parents were obtained. After the screening, 1,336 SNP markers were used to construct the linkage maps, resulting in a genetic map with a total length of 1955.7 cM and 16 linkage groups. The phenotypic and genotypic data were combined to conduct the quantitative trait locus (QTL) mapping analysis for seed numbers and fruit locule numbers. The results show that only one QTL for seed number was found in the spring trial 2023, which is closely linked to a molecular marker located at 45.2Mb of the second pair of chromosomes. This quantitative trait locus explained 20.03% percent of phenotypic variation. The same SNP marker is also the most closely linked marker to the fruit locule number QTL. Based on the above results, seed number is not a suitable indicator for evaluating tomato heat tolerance in this genetic mapping population. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2024-03-04T16:20:50Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2024-03-04T16:20:50Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員會審定書 ...I
致謝 ... II 摘要 ... III ABSTRACT ...IV 目次 ...VI 圖次 ...VIII 表次 ... X 縮寫表 ...XI 第一章 前言 ... 1 第一節 番茄概要 ...1 第二節 番茄與高溫逆境 ...1 第三節 番茄與單偽結果 ...4 第四節 番茄耐熱特性的數量性狀基因座定位 ....5 第二章 研究目的 ... 7 第三章 材料方法 ... 8 第一節 植物材料 ...8 第二節 外表型調查 ...8 第三節 基因型分型之定序文庫製備....10 第四節 定序資料分析與數量性狀基因座定位....16 第四章 結果 ... 19 第一節 栽種環境與外表型資料...19 第二節 基因型資料 ...28 第三節 數量性狀基因座定位 ...41 第五章 討論 ... 47 第一節 番茄開花、著果與種子數....47 第二節 基因型資料 ...48 第三節 數量性狀基因座定位 ...50 第六章 結論 ... 53 參考資料 ... 54 附錄 ... 64 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 番茄 | zh_TW |
| dc.subject | 長期和緩高溫 | zh_TW |
| dc.subject | 種子數目 | zh_TW |
| dc.subject | 數量性狀基因座 | zh_TW |
| dc.subject | seed number | en |
| dc.subject | Tomato | en |
| dc.subject | quantitative trait locus | en |
| dc.subject | long-term mild high temperature | en |
| dc.title | 以番茄重組自交族群 CLN4220 評估種子數目作為耐熱指標 | zh_TW |
| dc.title | Evaluation Seed Number as a Heat Tolerant Indicator in the tomato Recombinant inbred population CLN4220 | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 112-1 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 楊雯如;蔡育彰 | zh_TW |
| dc.contributor.oralexamcommittee | Wen-Ju Yang;Yu-Chang Tsai | en |
| dc.subject.keyword | 番茄,長期和緩高溫,種子數目,數量性狀基因座, | zh_TW |
| dc.subject.keyword | Tomato,long-term mild high temperature,seed number,quantitative trait locus, | en |
| dc.relation.page | 70 | - |
| dc.identifier.doi | 10.6342/NTU202400396 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2024-02-06 | - |
| dc.contributor.author-college | 生物資源暨農學院 | - |
| dc.contributor.author-dept | 農藝學系 | - |
| 顯示於系所單位： | 農藝學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-112-1.pdf 授權僅限NTU校內IP使用（校園外請利用VPN校外連線服務） | 4.3 MB | Adobe PDF |
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