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標題: | 水稻在過氧化氫處理下HEME ACTIVATOR PROTEIN 2J(OsHAP2J)對Os07g0122000的調控 Regulation of Os07g0122000 by HEME ACTIVATOR PROTEIN 2J (OsHAP2J) in rice (Oryza sativa) under hydrogen peroxide treatment |
作者: | 劉上源 Shang-Yuan Liu |
指導教授: | 鄭石通 Shih-Tong Jeng |
關鍵字: | 水稻,氧化逆境,miR156,NF-Ys,OsHAP2J,DUF1719,抗病機制, rice,oxidative stress,miR156,NF-Ys,OsHAP2J,DUF1719,disease response, |
出版年 : | 2024 |
學位: | 碩士 |
摘要: | 自然環境中,植物無時無刻面對著各種生物或非生物性逆境,並啟動防禦機制以作出應對。當面臨外界壓力時,植物體內會產生活性氧化物(Reactive Oxygen Species,ROS),如:過氧化氫(Hydrogen peroxide,H2O2)與氫氧自由基(Hydroxyl radical,OH•)等。過多的ROS會使植物面臨氧化逆境;然而,ROS也同時可作為二次訊息分子,啟動下游基因調控。實驗室先前研究主要關注在H2O2處理所導致的氧化逆境下,14天大水稻幼苗根部的訊息傳遞。實驗室前人建立了三筆水稻資料庫:small RNA deep sequencing data、degradome及transcriptome。利用這些資料並加以驗證,發現H2O2的處理在短時間內降低了microRNA156(miR156)的表達量,並導致其目標基因SQUAMOSA promoter-binding-like protein 2(OsSPL2)的上調。此外,OsSPL2會作為負調控者,抑制其下游基因Heme Activator Protein 2J(OsHAP2J)的表現。OsHAP2J又被稱為nuclear factor YA5 (OsNF-YA5),屬於nuclear factor Y(NF-Y)家族。NF-YA(YA)與NF-YB(YB)和NF-YC(YC)會形成三聚體(heterotrimer),並通過YA的DNA結合域,結合下游基因啟動子中的CCAAT-box來加以調控。本研究的目標是找到OsHAP2J之調控模型與其互動蛋白,YB與YC,並通過建立和交叉比較各轉錄體資料庫來探索OsHAP2J的目標基因。藉由這些研究策略發現,OsHAP2J可能抑制一個具有未知功能域(domain of unknown function,DUF)DUF1719的蛋白基因Os07g0122000,且該基因可能與水稻的抗病機制有關。綜合這些結果,推測在H2O2處理下,miR156所調控的下游途徑會影響水稻的抗病性。 Plants respond to biotic or abiotic stresses all the time. When facing stresses, plants produce reactive oxygen species (ROS), such as hydrogen peroxide (H2O2) and hydroxyl radical (HO•). Excessive ROS causes plants to encounter oxidative stress, but at the same time, ROS can also serve as a secondary signal to initiate downstream regulations. Our previous studies mainly focused on the signaling pathway within 14-day-old rice root under H2O2-treated oxidative stress. Several databases, including small RNA deep sequencing, degradome, and transcriptome, were built in rice. Based on these databases and further validation, the expression of microRNA 156 (miR156) was decreased in rice treated with H2O2, and caused the upregulation of miR156’s target gene, SQUAMOSA promoter-binding-like protein 2 (OsSPL2). In addition, OsSPL2, acting as a negative regulator, suppresses the expression of its downstream gene, Heme Activator Protein 2J (OsHAP2J). OsHAP2J is a nuclear factor YA5 (OsNF-YA5) that belongs to the Nuclear Factor Y (NF-Y) family. NF-YA (YA) forms a heterotrimer with NF-YB (YB) and NF-YC (YC), and binds to the CCAAT-box of downstream genes through the DNA binding domain of YA. Here, the regulatory module of OsHAP2J was investigated and its interaction partners, YB and YC, was screened by yeast-two hybridization. This study suggested that OsHAP2J may suppress the expression of Os07g0122000 encoding a domain called DUF1719, and Os07g0122000 may be associated with disease resistance in rice. Hence, we hypothesized that the molecular mechanism regulated by miR156 affects the disease resistance of rice under H2O2 treatment. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/91792 |
DOI: | 10.6342/NTU202400514 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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