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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳俊顯(Chun-hsien Chen) | |
| dc.contributor.author | Chiu-Feng Chen | en |
| dc.contributor.author | 陳秋楓 | zh_TW |
| dc.date.accessioned | 2021-05-20T20:10:13Z | - |
| dc.date.available | 2014-08-04 | |
| dc.date.available | 2021-05-20T20:10:13Z | - |
| dc.date.copyright | 2009-08-04 | |
| dc.date.issued | 2009 | |
| dc.date.submitted | 2009-07-29 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/9128 | - |
| dc.description.abstract | 金奈米粒子(gold nanoparticles，GNPs)有很強的表面電漿共振吸收(surface plasmon resonance，SPR)，其吸收峰位置會隨粒子間距改變而產生位移的現象，故藉由金奈米粒子間的分散─聚集狀態所產生的紅色─藍色之顏色變化可應用於目測辨識之比色法偵測。
本論文要介紹的是利用官能基間的協力效應(cooperative effect)所產生之催化酯化及催化水解反應的方法，並以此方法偵測人體肌肉收縮代謝之最終產物─肌酸酐(creatinine)。肌酸酐會藉由腎絲球的排泄而排入尿液中，且在正常人的尿液中之濃度恆定，因此藉由檢測尿液中的肌酸酐濃度可粗篩出由腎絲球病變所引起的腎臟疾病。我們利用兩階段的官能基置換方式依序在金奈米粒子表面修飾thioctic acid (TA)及([15]crown-5)CH2O(CH2)4SH (簡寫成15c5-C4-SH)兩種官能基，稱之為15c5-C4-S/TA-GNP。利用酯化反應使15c5-C4-S/TA-GNP在溶液中呈現穩定分散的紅色，而添加肌酸酐(creatinine)後，酯化之15c5-C4-S/TA-GNP因進行水解反應而產生聚集的藍色，藉由金奈米粒子由分散到聚集所產生的顏色變化以偵測肌酸酐。15c5-C4-S/TA-GNPs的酯化反應是在pH 2~5的甲醇溶液(含有10%水)中進行，此時金奈米粒子會因為TA官能基上的羧酸基酯化成甲酯基，而15c5官能基則是藉由抓住溶液中的鈉離子來提供正電斥力，故金奈米粒子能藉由兩官能基間的協力效應而穩定分散於溶液中，溶液呈現紅色；相對地，在酯化反應的控制實驗中，金奈米粒子會因為TA上的羧酸基間產生的氫鍵吸引而聚集，溶液呈現藍色。最後將含有肌酸酐的樣品加入含已酯化之金奈米粒子的溶液中，此時15c5與肌酸酐上之質子化的胺基作用，使得鄰近於15c5之TA上的甲酯基能接受質子化的胺基所釋出之質子而進行酸催化的水解反應，令TA上的甲酯基因水解而回復成羧酸基，因此金奈米粒子間會因氫鍵的生成而聚集，溶液呈現藍色，故此法可簡易地直接以肉眼觀察到的顏色變化來做快速的腎臟疾病篩檢。 | zh_TW |
| dc.description.abstract | Gold nanoparticles (GNPs) exhibit intense red color due to the surface plasmon absorbance whose peak position is sensitive to the interparticle distance. By taking advantage of the color transition of red to blue associated with the dispersive to aggregated states of GNPs, many colorimetric sensing strategies have been developed.
Presented here is a novel approach that cooperatively catalytic esterification and hydrolysis are involved. We examine the model system using creatinine, which is a metabolite of muscle contraction and is cleared from the body by kidney via glomerular filtration. The concentrations of the serum or urinary creatinine are almost constant for healthy people such that the creatinine levels are used as an indicator to evaluate kidney function. GNPs are first bifunctionalilzed by ([15]crown-5)CH2O(CH2)4SH (denoted 15c5-C4-SH) and thioctic acid (TA). Secondly, esterification takes place and stabilizes GNPs when the bifunctionalized 15c5-C4-S/TA-GNPs are dissolved in a pH 2~5 methanol (containing ~10% water). In control experiments without esterification, hydrogen bonding at the carboxylic-moiety drives aggregation and destabilizes GNPs. For the sensing examination, creatinine is introduced in to esterified GNPs and a cooperatively catalytic hydrolysis leads to the aggregation of GNPs via the developed interparticle hydrogen bonding. Ultimately, rapid evaluation of kidney function by the naked-eye is successful through colorimetric sensing of creatinine with the use of the bifunctionalized-gold nanoparticle assay. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-20T20:10:13Z (GMT). No. of bitstreams: 1 ntu-98-R96223124-1.pdf: 6172030 bytes, checksum: 9b24a846cb237354192fe17534db688e (MD5) Previous issue date: 2009 | en |
| dc.description.tableofcontents | 中文摘要 I
英文摘要 II 謝誌 III 目錄 IV 圖目錄 VII 表目錄 X 第一章、 緒論 1 1-1 肌酸酐簡介 1 1-1-1 肌酸酐的生理特性與臨床意義 1 1-1-1-1 肌酸酐測定方法的比較 2 1-1-1-2 Jaffé反應法 2 1-1-1-3 酵素法 3 1-2 奈米粒子簡介 4 1-3 奈米金的光學性質 4 1-4 奈米金的製備方法 9 1-4-1 水相檸檬酸鹽還原法 10 1-4-2 兩相法 11 1-5 奈米金的表面修飾方法 12 1-6 奈米金的分散與聚集 14 1-7 奈米金在感測器的應用 15 1-7-1 DNA感測器的應用 15 1-7-2 分子感測器的應用 19 1-7-3 金屬離子感測器的應用 20 1-8 研究動機 26 第二章、 實驗部份 27 2-1 藥品與器材 27 2-1-1 化學藥品 27 2-1-2 實驗器材 28 2-2 實驗儀器設備 29 2-3 15c5-C4-SH的製備 30 2-3-1 15c5-C4-Br的合成 30 2-3-2 15c5-C4-SH的合成 30 2-4 15c5-C12-SH的製備 31 2-4-1 15c5-C12-Br的合成 31 2-4-2 15c5-C12-SH的合成 31 2-5 金奈米粒子的製備與表面修飾 32 2-5-1 Citrate-GNPs的製備 32 2-5-2 15c5-C4-S/TA-GNPs的合成 32 2-5-2-1 第一階段修飾：TA-GNPs的合成 33 2-5-2-2 第二階段修飾：15c5-C4-S/TA-GNPs的合成 33 2-5-3 15c5-C12-S/TA-GNPs的合成 34 2-6 實驗流程與溶液配製 34 2-6-1 實驗流程 34 2-6-2 溶液配製 35 2-6-2-1 分散之奈米金溶液 35 2-6-2-2 肌酸酐偵測溶液 35 2-7 儀器量測 35 2-7-1 UV-vis光譜量測 35 2-7-2 IR光譜量測 36 2-7-2-1 金膜玻璃片的製備 36 2-7-2-2 樣品製備及光譜量測 37 2-7-3 粒徑與介面電位量測 37 2-7-3-1 粒徑量測 37 2-7-3-2 介面電位量測 38 第三章、 結果與討論 40 3-1 肌酸酐之目測辨識與定量 40 3-2 分散與聚集之奈米金的機制探討 43 3-2-1 分散之奈米金的機制探討 43 3-2-1-1 羧酸官能基間的氫鍵引力 43 3-2-1-2 15c5-C4-S/TA-GNPs的酯化 44 3-2-1-3 使酯化之15c5-C4-S/TA-GNPs穩定分散的斥力來源 46 3-2-1-4 TA與15冠5醚間的協力效應對酯化反應的影響 52 3-2-2 聚集之奈米金的機制探討 54 3-2-2-1 酯化之15c5-C4-S/TA-GNPs的水解 54 3-2-2-2 肌酸酐對水解反應的影響 55 3-2-2-3 TA與15冠5醚間的協力效應對水解反應的影響 58 3-2-2-3-1 冠醚孔徑大小對水解的影響 59 第四章、 結論 63 第五章、 參考文獻 65 | |
| dc.language.iso | zh-TW | |
| dc.title | 含冠醚與羧酸之奈米金於目測辨識肌酸酐之研究：相鄰官能基對酯化及水解之協力催化反應 | zh_TW |
| dc.title | Colorimetric Sensing of Creatinine by Cooperative Catalysis of Esterification and Hydrolysis on Bifunctionalized Gold Nanoparticles | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 97-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳昭岑(Chao-Tsen Chen),邱勝賢(Sheng-Hsien Chiu) | |
| dc.subject.keyword | 奈米金,協力催化反應,比色法偵測,肌酸肝, | zh_TW |
| dc.subject.keyword | Gold Nanoparticles,Cooperative Catalysis,Colorimetric Sensing,Creatinine, | en |
| dc.relation.page | 69 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2009-07-29 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 化學研究所 | zh_TW |
| 顯示於系所單位： | 化學系 | |
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