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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 張麗冠 | zh_TW |
| dc.contributor.advisor | Li-Kwan Chang | en |
| dc.contributor.author | 張舒茵 | zh_TW |
| dc.contributor.author | Shu-Yin Chang | en |
| dc.date.accessioned | 2023-10-03T16:54:54Z | - |
| dc.date.available | 2023-11-09 | - |
| dc.date.copyright | 2023-10-03 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-08-04 | - |
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Comprehensive analyses of intraviral Epstein-Barr virus protein -protein interactions hint central role of BLRF2 in the tegument network. J Virol. Zandi, R., and Reguera, D. (2005). Mechanical properties of viral capsids. Phys Rev E 72, 021917. Zhu, H.Y., Yamada, H., Jiang, Y.M., Yamada, M., and Nishiyama, Y. (1999). Intracellular localization of the UL31 protein of herpes simplex virus type 2. Arch Virol 144, 1923-1935. Zimmermann, J., and Hammerschmidt, W. (1995). Structure and role of the terminal repeats of Epstein-Barr virus in processing and packaging of virion DNA. J Virol 69, 3147-3155. 陳澔 (2022). Epstein-Barr virus proteins interacting with BGLF1. National Taiwan University Master Thesis. | - |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/90625 | - |
| dc.description.abstract | Epstein-Barr virus為第四型人類皰疹病毒，同時也是第一個被發現與人類癌症相關的病毒，並且全球九成的人口被 EB 病毒感染。EB 病毒的生活史可以分成潛伏期 (latency) 以及溶裂期 (lytic cycle) ，在潛伏期只會有少量的病毒基因表現，以維持病毒基因體保留在宿主細胞中，而在經過適當的刺激後，會使 EB 病毒進入溶裂期，產生成熟的病毒顆粒從宿主細胞釋放。目前已知病毒顆粒的 Capsid-associated tegument complex (CATC) 對於病毒殼體的穩定性及病毒顆粒出核 (nuclear egress) 具有重要功能。而 EB 病毒的 CATC 是由 BGLF1、BPLF1 及 BVRF1 所組成，本研究室先前的研究發現 BGLF1 除了能與殼體蛋白結合之外，並且能與在出核過程中扮演重要角色的 BFLF2 結合，因此推測 BGLF1 可能會參與出核的過程，不過詳細的機制尚不清楚。為了進一步釐清 BGLF1 在出核過程中跟相關蛋白質之間的結合，本研究透過 GST pull-down assay 證明 BGLF1 與 BFLF2 和 BFRF1 之間是直接結合，並且透過免疫螢光染色發現在 nuclear egress complex (NEC) 存在的情況下，會共定位在核膜。接著為了確認在出核過程中，procapsid 是否必須藉由 BGLF1 與 NEC 之間的結合，才能將其順利移動到核膜，同樣藉由 GST pull-down assay 釐清殼體蛋白與組成 NEC 的蛋白質之間的結合情形，結果發現組成病毒殼體的蛋白質包含 VCA、BFRF3、BDLF1 及 BORF1，在體外條件下皆能直接與 BFLF2 和 BFRF1 結合。另外本研究透過免疫螢光染色觀察帶有標籤的 BGLF1、BFLF2、BFRF3 以及 Rta 在 HeLa 細胞的分佈，確認在細胞內 BGLF1 與 BFLF2、BFRF3 會在細胞質共定位，而與 Rta 在細胞核以及細胞質共定位。希望透過本研究能幫助釐清 BGLF1 與其他病毒蛋白的結合關係，進一步推測 BGLF1 在 EB 病毒出核時所扮演的角色。 | zh_TW |
| dc.description.abstract | Epstein-Barr virus (EBV) is a type 4 human herpesvirus and the first virus discovered to be associated with human cancers. It infects approximately 90% of the global population. The life cycle of EBV can be divided into latency and lytic phase. During latency, only few viral genes are expressed to maintain viral genome replication. Upon appropriate stimulation, EBV enters the lytic phase, leading to the production and release of mature viral particles from host cells. Capsid-associated tegument complex (CATC) have been found to play important roles in stabilizing the viral capsid and facilitating nuclear egress of viral particles. The CATC proteins of EBV include BGLF1, BPLF1, and BVRF1. Previous study in our laboratory has shown that BGLF1 not only interacts with capsid proteins but also associates with BFLF2, which plays a crucial role in the nuclear egress. This suggests that BGLF1 is involved in the nuclear egress process, although the detailed mechanism remains unclear. To further elucidate the interactions between BGLF1 and relevant proteins during nuclear egress, this study implemented GST pull-down assays to demonstrate direct interactions between BGLF1 and BFLF2 as well as BFRF1. Immunofluorescence analysis revealed that BGLF1 colocalizes with nuclear egress complex (NEC) at the nuclear membrane. Then, in order to confirm whether BGLF1 is indispensable for recruitment of procapsid to the nuclear membrane during nuclear egress, GST pull-down assays indicated the interactions between capsid proteins and NEC components. The results showed that viral capsid proteins including VCA, BFRF3, BDLF1, and BORF1 directly interact with BFLF2 and BFRF1 under in vitro conditions. Additionally, this study examined the distribution of tagged BGLF1, BFLF2, BFRF3, and Rta in HeLa cells through immunofluorescence analysis and found that BGLF1 colocalized with BFLF2 as well as BFRF3 in the cytoplasm, while colocalized with Rta in both the nucleus and cytoplasm. Collective of this study clarifies the interactions between BGLF1 and other viral proteins, further elucidating the role of BGLF1 in EBV nuclear egress. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-10-03T16:54:54Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-10-03T16:54:54Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 中文摘要 iii ABSTRACT iv 目錄 vi 表目錄 ix 圖目錄 x 第一章 前言 1 1.1 Epstein-Barr virus (EB 病毒) 1 1.1.1 EB 病毒的簡介 1 1.1.2 EB 病毒的遺傳物質與基因命名 1 1.1.3 EB 病毒的生命史 2 1.2 單純皰疹病毒 (Herpesvirus simplex virus, HSV) 的殼體產生與成熟 4 1.2.1 HSV 殼體的結構與組裝 4 1.2.2 HSV 的間層蛋白質 (tegument protein) 5 1.2.3 HSV 的 capsid vertex-specific component (CVSC) 6 1.2.4 EB 病毒殼體的結構與組裝 7 1.2.5 EB病毒的間層蛋白質 (tegument protein) 8 1.2.6 EB 病毒的 capsid associated tegument complex (CATC) 8 1.2.7 皰疹病毒的殼體出核 (nuclear egress) 10 1.2.8 EB 病毒的殼體出核 12 1.3 BGLF1 13 1.4 研究動機 13 第二章 材料與方法 15 2.1 細胞株與細胞培養 15 2.1.1 HEK293T 15 2.1.2 HeLa cell 15 2.2 菌株與細菌培養 16 2.3 質體與質體建構 16 2.4 質體DNA萃取 (Plasmid extraction) 18 2.5 轉染 (Transfection) 19 2.6 勝任細胞 (Competent cells) 的製備 19 2.7 轉型作用 (Transformation) 19 2.8 快篩 (Quick Screen) 20 2.9 共免疫沉澱 (Co-immunoprecipitation assay) 20 2.10 穀胱甘肽 S- 轉移酶沉降分析 (GST pull-down) 21 2.11 西方墨點法 (Western bolt, immunoblotting, IB) 22 2.12 免疫螢光染色 (Immunofluorescence, IF) 23 第三章 結果 25 3.1 BGLF1 與病毒蛋白質在細胞的分佈 25 3.1.1 BGLF1 在 HeLa 細胞的分佈模式 25 3.1.2 BGLF1 與 BFLF2 在 HeLa 共定位於細胞質 25 3.1.3 BGLF1 與 BFRF3 在 HeLa 細胞共定位於細胞質 26 3.1.4 BGLF1 與 Rta在 HeLa 細胞的分佈 26 3.2 BGLF1 會與 BFLF2 和 BFRF1 直接結合 27 3.2.1 BGLF1 會與 NEC 共定位在核膜附近 28 3.3 殼體蛋白質 與 BFLF2、BFRF1 之間的結合 28 3.3.1 BFRF3 與 BFLF2 或BFRF1 的結合 28 3.3.2 BDLF1 與 BFLF2 或 BFRF1 的結合 29 3.3.3 BORF1 與 BFLF2 或 BFRF1 的結合 30 3.3.4 VCA 與 BFLF2 的結合 30 3.3.5 Rta 與 BFLF2、BFRF1 的結合 31 第四章 討論 33 4.1 BGLF1 在殼體出核中扮演的角色 33 4.2 BGLF1 與 NEC 共定位 35 4.3 BGLF1 與 BFLF2 以及 BFRF3 在 HeLa 細胞的共定位 35 4.4 Rta 可能改變 BGLF1 在細胞中的分佈 36 4.5 CATC 在 EB 病毒的功能 37 4.6 HCMV 與 EB 病毒的相似性 37 4.7 EB 病毒殼體出核相關病毒蛋白質結合模型圖 38 4.8 結論 39 第五章 圖表 40 參考文獻 63 附錄 74 | - |
| dc.language.iso | zh_TW | - |
| dc.title | EB病毒之BGLF1與出核相關蛋白質的結合 | zh_TW |
| dc.title | Interaction of Epstein-Barr virus BGLF1 with proteins involved in nuclear egress | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 111-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 劉世東;陳美如;李重霈;吳育騏 | zh_TW |
| dc.contributor.oralexamcommittee | Shih-Tung Liu;Mei-Ru Chen;Chung-Pei Lee;Yu-Chi Wu | en |
| dc.subject.keyword | EB病毒,BGLF1,出核(nuclear egress),BFLF2,BFRF1,殼體組裝(capsid assembly), | zh_TW |
| dc.subject.keyword | Epstein-Barr virus,BGLF1,nuclear egress,BFLF2,BFRF1,capsid assembly, | en |
| dc.relation.page | 78 | - |
| dc.identifier.doi | 10.6342/NTU202302766 | - |
| dc.rights.note | 同意授權(限校園內公開) | - |
| dc.date.accepted | 2023-08-08 | - |
| dc.contributor.author-college | 生命科學院 | - |
| dc.contributor.author-dept | 生命科學系 | - |
| Appears in Collections: | 生命科學系 | |
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| ntu-111-2.pdf Restricted Access | 3.46 MB | Adobe PDF | View/Open |
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