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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 鄭景暉 | zh_TW |
| dc.contributor.advisor | Jiiang-Huei Jeng | en |
| dc.contributor.author | 劉立偉 | zh_TW |
| dc.contributor.author | Li-Wei Liu | en |
| dc.date.accessioned | 2023-09-26T16:19:07Z | - |
| dc.date.available | 2023-11-10 | - |
| dc.date.copyright | 2023-09-26 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-07-13 | - |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/90335 | - |
| dc.description.abstract | 丁酸 (Butyric acid)屬於短鏈脂肪酸(Short Chain Fatty Acids)的一種，是牙髓以及牙周致病菌代謝纖維食物後之產物，且已經被文獻提出會在受感染的牙髓及根管系統及牙齦溝液中被測得相當之濃度，且證實與牙髓炎／牙根尖炎及牙周炎的發生與進行有相當之關聯；人類血管內皮細胞(human vascular endothelial cell)被視為與血管新生、牙髓及牙周組織的發炎和癒合扮演重要角色；然而丁酸是否會誘發血管內皮細胞的發炎反應、細胞自噬、凝血反應以及血管鈣化，進而在牙髓炎與牙周炎致病機轉之角色，到目前為止鮮少有研究與討論，也是本研究之重要目的。 | zh_TW |
| dc.description.abstract | Butyric acid, as one kind of short chain fatty acids (SCFAs) and is mainly secreted as a metabolic product of fibrous food by the bacteria in the periodontal pocket/pulpal-root canal. It has been reported to be elevated to significant concentrations in the contaminated root canal systems, as well as in the gingival crevicular fluid. Higher SCFAs are confirmed to be significantly associated with the occurrence and progression of pulpitis/apical periodontitis and periodontitis. Human vascular endothelial cells are considered to play an important role in angiogenesis, inflammation, and healing of dental pulp and periodontal tissue. However, whether butyrate induces inflammatory responses, autophagy, coagulation, and vascular calcification in endothelial cells and its role in the pathogenesis of pulpitis and periodontitis has been rarely studied and discussed, and is also an important objective of this study. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-09-26T16:19:07Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-09-26T16:19:07Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 中文摘要 i
Abstract iii Table of Contents vi List of Tables xiii Chapter I Literature Review 1 1.1 The relationship between dental pulp and vascular endothelia cell 1 1.2 EA.hy926 endothelial cell 2 1.2.1 Mechanism of inflammatory response of vascular endothelial cell 3 1.2.2 Mechanism of autophagy- response of vascular endothelial cell 4 1.2.3 Mechanism of mineralization response of vascular endothelial cell 5 1.2.4 Mechanism of coagulation response of vascular endothelial cell 7 1.3 Butyrate (Butyric acid) 8 1.3.1The effects of butyrate on dental pulp tissue and periodontal/periapical area 10 1.4 The relationship between dental pulp inflammation and regeneration/repair 11 Chapter II Research Purposes and Hypothesis 12 Chapter III Materials and Methods 13 3.1 Materials preparation 13 3.2 Observation of cell morphology 14 3.3 MTT assay 14 3.4 Culture of EA.hy926 endothelial cells 15 3.5 Real-time quantitative polymerase chain reaction (rt-qpcr) 17 3.5.1 RNA extraction 17 3.5.2 RNA quantification 18 3.5.3 Reverse transcription 19 3.6 Immunofluorescent (IF) staining 21 3.7 Data analysis 22 Chapter IV Results 23 4.1 EA.hy926 endothelial cell morphological observation 23 4.2 Effects of butyrate on cell viability of EA.hy926 endothelial cell - MTT assay 23 4.3 Effects of butyrate on inflammation-related genes and proteins of EA.hy926 endothelial cells – RT-PCR and immunofluorescence 24 4.4 Effects of butyrate on autophagy-related genes and proteins of the EA.hy926 endothelial cells–RT-PCR and immunofluorescence 27 4.5 Effects of butyrate on coagulation-related genes and proteins of the EA.hy926 endothelial cells–RT-PCR and immunofluorescence 28 4.6 Effects of butyrate on mineralization-related genes and proteins of the EA.hy926 endothelial cells–RT-PCR and immunofluorescence 29 Chapter V Discussion 31 5.1 Effect of butyrate on inflammation related genes of EA.hy926 endothelial cells 31 5.2 Effect of butyrate on autophagy related genes of EA.hy926 endothelial cells 38 5.3 Effect of butyrate on coagulation related genes of EA.hy926 endothelial cells 39 5.4 Effect of butyrate on mineralization related genes of EA.hy926 endothelial cells 40 Chapter VI Conclusion 43 References 44 Figures 50 Figure 1. The chemical structure of butyrate 50 Figure 2. MTT assay of EA.hy926 endothelial cells with various concentrations of butyrate (NC, 1, 2, 4, 8, 16 mM) for 3 days, n=55 50 Figure 3. Morphological observation of EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 51 Figure 4. Expression of IL-1 alpha in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 52 Figure 5. Expression of IL-1 beta in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 53 Figure 6. Expression of IL-18 in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 54 Figure 7. Expression of COX-2 in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 55 Figure 8. Expression of cPLA-2 in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 56 Figure 9. Expression of sPLA-2 in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 57 Figure 10. Expression of uPA in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 58 Figure 11. Expression of uPAR in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 59 Figure 12. Expression of HO-2 in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 60 Figure 13. Expression of N-cadherin in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 61 Figure 14. Expression of iNOS in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 62 Figure 15. Expression of SPARC in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 63 Figure 16. Expression of Vimentin in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 64 Figure 17. Expression of ATG12 in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 65 Figure 18. Expression of LC3B in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 66 Figure 19. Expression of Beclin-1 in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 67 Figure 20. Expression of TF in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 68 Figure 21. Expression of alpha2 antiplasmin in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 69 Figure 22. Expression of PAI-1 in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 70 Figure 23. Expression of osteocalcin in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 71 Figure 24. Expression of osteoactivin in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 72 Figure 25. Expression of tPA in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 73 Figure 26. Expression of VE-cadherin in EA.hy926 cells with butyrate at various concentrations for 1 day- Real-time PCR data 74 Figure 27. Immunofluorescent staining of IL-1 alpha treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 75 Figure 28. Immunofluorescent staining of IL-1 beta treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 76 Figure 29. Immunofluorescent staining of IL-18 treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 77 Figure 30. Immunofluorescent staining of COX-2 treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 78 Figure 31. Immunofluorescent staining of p-cPLA-2 treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 79 Figure 32. Immunofluorescent staining of sPLA-2 treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 80 Figure 33. Immunofluorescent staining of uPAR treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 81 Figure 34. Immunofluorescent staining of HO-1 treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 82 Figure 35. Immunofluorescent staining of VE-cadherin treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 83 Figure 36. Immunofluorescent staining of VCAM-1 treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 84 Figure 37. Immunofluorescent staining of SPARC treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 85 Figure 38. Immunofluorescent staining of vimentin treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 86 Figure 39. Immunofluorescent staining of ATG12 treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 87 Figure 40. Immunofluorescent staining of LC3Btreated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 88 Figure 41. Immunofluorescent staining of Beclin-1 treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 89 Figure 42. Immunofluorescent staining of TF treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) ) 90 Figure 43. Immunofluorescent staining of alpha2 antiplasmintreated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 91 Figure 44. Immunofluorescent staining of PAI-1 treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 92 Figure 45. Immunofluorescent staining of OSX treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 93 Figure 46. Immunofluorescent staining of osteoactivin treated with various concentrations of butyrate on EA.hy926 cells for 24 hrs (400x magnification) 94 Tables 95 | - |
| dc.language.iso | en | - |
| dc.subject | 丁酸 | zh_TW |
| dc.subject | 基因表現 | zh_TW |
| dc.subject | 鈣化 | zh_TW |
| dc.subject | 凝血 | zh_TW |
| dc.subject | 細胞自噬 | zh_TW |
| dc.subject | 發炎介質 | zh_TW |
| dc.subject | 人類血管內皮細胞 | zh_TW |
| dc.subject | EA.hy926 endothelial cells | en |
| dc.subject | Butyrate (Butyric acid) | en |
| dc.subject | gene expression | en |
| dc.subject | mineralization | en |
| dc.subject | cell autophagy | en |
| dc.subject | coagulation | en |
| dc.subject | inflammation mediator | en |
| dc.title | 丁酸對血管內皮細胞發炎介質、細胞自噬、凝血與鈣化相關基因與蛋白表現之影響 | zh_TW |
| dc.title | Effect of butyrate on the expression of various inflammatory mediators, autophagy-, coagulation- and mineralization-related genes and proteins in vascular endothelial cells | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 111-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 何元順;張美姬;陳莉菁;蔡宜玲 | zh_TW |
| dc.contributor.oralexamcommittee | Yuan-Soon Ho;Mei-Chi Chang;Li-Ching Chen;Yi-Ling Tsai | en |
| dc.subject.keyword | 丁酸,人類血管內皮細胞,發炎介質,細胞自噬,凝血,鈣化,基因表現, | zh_TW |
| dc.subject.keyword | Butyrate (Butyric acid),EA.hy926 endothelial cells,inflammation mediator,cell autophagy,coagulation,mineralization,gene expression, | en |
| dc.relation.page | 97 | - |
| dc.identifier.doi | 10.6342/NTU202301551 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2023-07-13 | - |
| dc.contributor.author-college | 醫學院 | - |
| dc.contributor.author-dept | 臨床牙醫學研究所 | - |
| Appears in Collections: | 臨床牙醫學研究所 | |
Files in This Item:
| File | Size | Format | |
|---|---|---|---|
| ntu-111-2.pdf Restricted Access | 81.1 MB | Adobe PDF |
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