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標題: | 運用新穎的的光電晶體生物感測器在高鹽度環境中檢測多巴胺 A New Phototransistor Biosensor for Dopamine Detection in High Salinity Environments |
作者: | 吳宗翰 TsungHan Wu |
指導教授: | 陳逸聰 Yit-Tsong Chen |
關鍵字: | 場效電晶體生物感測器,光電晶體生物感測器,上轉換奈米粒子,德拜屏蔽效應,二硫化錫奈米薄片, FET biosasensor,phototransistor biosensor,upconverting nanoparticle,Debye screening effect,Tin disulfide nanosheets, |
出版年 : | 2023 |
學位: | 碩士 |
摘要: | 在生物感測領域中,場效電晶體(Field-Effect Transistor, FET)的使用已廣泛應用於各種生物分子的檢測。然而,傳統的FET生物感測器在高離子強度的環境中常受到德拜屏蔽效應(Debye Screening Effect)的影響,導致其偵測能力大幅下降。在這篇論文研究中,我們設計了一種新型的光電晶體(phototransistor)生物感測器,成功地突破了此一限制。我們使用的光電晶體採用二硫化錫作為通道材料,並將上轉換奈米粒子(Upconverting Nanoparticle ,UCNP)與適體(Aptamer)結合,使其能夠調控光電流在高離子強度的環境中準確偵測目標分子。UCNP受980 nm雷射激發時會發出530 nm的綠光,且此綠光被二硫化錫吸收產生光電流,當目標分子與適體結合,會改變其結構並拉近UCNP與FET的距離帶,以帶來光電流的變化,而這種變化較不會受到德拜屏蔽效應的影響。我們以多巴胺作為目標進行測量,實驗結果證明,在不照光時,在1倍磷酸鹽緩衝溶液中(1x Phosphate Buffered Saline,1x PBS)中的偵測極限達到10 fM,而在血清中也達到了100 fM。但在光強度為215 nW/µm2時,其偵測極限在1x PBS中可達10 aM,在血清中則為100 aM,超過傳統FET生物感測器的偵測能力。此外,偵測範圍也隨著光強度的增加而擴大,同時由於訊號的增強解析度也有提高,顯示出其卓越的適應性和靈活性。這些成果展現了光電晶體作為生物流體內實時檢測生物分子的強大潛力。我們期待光電晶體在未來可以廣泛應用於高鹽環境中的人體檢體,如檢測生物標記物於血液和尿液等。 In the realm of biosensing, the use of field-effect transistors (FETs) has been broadly applied for the detection of a variety of biomolecules. However, traditional FET Biosensors are often affected by the Debye screening effect in high ionic strength environments, leading to a significant decrease in their detection capabilities. In this study, we designed a novel phototransistor biosensor that can successfully overcome this limitation. This phototransistor employs tin disulfide nanosheets as channel material and integrates upconverting nanoparticles (UCNPs) with aptamers (referred as UCNP/Apt/SnS2-FET), allowing accurate detection of target molecules in high ionic strength environments. When excited by a 980 nm laser, the UCNPs emit at the green light 530 nm which is absorbed by tin disulfide nanosheets to generate a photocurrent. As the target molecule binds with the aptamer, it changes its structure and brings the UCNP closer to the FET, resulting in a change in photocurrent of the UCNP/Apt/SnS2-FET that is not significantly influenced by the Debye screening effect. Using dopamine as the target for measurement, experimental results demonstrated that, without 980 nm illumination, the detection limit reached 10 fM in 1x PBS and 100 fM in serum. In contract, with the light intensity of 215 nW/µm² at 980 nm, the detection limit in 1x PBS could reach 10 aM, and 100 aM in serum, exceeding the detection capabilities of traditional FET Biosensors. In addition, the linear working range of a UCNP/Apt/SnS2-FET expanded with increasing light intensity, and the signal resolution was enhanced, demonstrating its excellent adaptability and flexibility. These achievements display the powerful potential of the phototransistor for real-time detection of biomolecules within biofluids. We anticipate the phototransistor can be widely applied to detwct biomarkers in high-salt human biofluids (such as blood and urine) in the future. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/90112 |
DOI: | 10.6342/NTU202304043 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 化學系 |
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