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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 楊燿州 | zh_TW |
| dc.contributor.advisor | Yao-Joe Yang | en |
| dc.contributor.author | 洪笙峰 | zh_TW |
| dc.contributor.author | Sheng-Feng Hung | en |
| dc.date.accessioned | 2023-09-22T17:04:47Z | - |
| dc.date.available | 2023-11-09 | - |
| dc.date.copyright | 2023-09-22 | - |
| dc.date.issued | 2023 | - |
| dc.date.submitted | 2023-08-11 | - |
| dc.identifier.citation | T. M. Keenan and A. Folch, “Biomolecular gradients in cell culture systems,” Lab on a Chip, vol. 8, no. 1, pp. 34-57, 2008.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/90019 | - |
| dc.description.abstract | 本研究開發剪應力梯度產生器(shear stress gradient generators, SSGG)，與提出SSGG與濃度梯度產生器(Concentration gradient generator, CGG)及軟性感測晶片之整合技術。CGG是使用單步稀釋的微流道網路設計，並能夠在寬廣的輸入流量範圍內表現出高通量之特性。合成CGG的方法是利用電路與微流道網路之間的類比計算出混合流道的等效流阻，透過每個混合流道之間的流阻差異，能夠控制樣本液與緩衝液流入各個混合流道的體積混和比，並進一步在微流道出口產生預定義之濃度分布，透過此方法成功生成了線性(Linear)之濃度梯度。設計的SSGG原理是根據使用者定義之剪應力比例而計算出相對應的流道長度。濃度梯度產生器整合元件和剪應力梯度產生器整合元件皆透過微影製程(Photolithography)技術將其製成三層的微流道結構，並將三層PDMS層與軟性感測器整合。CGGs是透過將樣本液與緩衝液通入元件中進行量測，量測結果與理論值的誤差在5%之內，且在1.8至18 µL/min的流量範圍內能夠維持穩定的濃度梯度分布。SSGGs是透過量測液體在出口毛細管中的流速以計算出剪應力，線性和二次對數(2-fold logarithmic)之SSGGs的量測結果與理論值的誤差皆在5%之內，且在13 µL/min的流量範圍內能夠維持穩定。 | zh_TW |
| dc.description.abstract | This work presents the integration of gradient concentration generators (CGGs), shear stress gradient generators (SSGGs), and fluidic sensing devices. The CGGs, which can be synthesized by using a proposed algorithm, exhibits high throughput with a wide range of input flowrates. The proposed CGG-synthesizing algorithm calculates the equivalent channel flow resistances, which control the volumetric mixing ratios of the CGG mixing channels to generate predefined concentration gradient profiles. The optimization of the flow resistance ratios for realizing smallest footprint of microfluidic chips was performed in this work. In addition, microfluidic networks for generating various shear stress gradients were also designed and implemented. The SSGGs regulate fluid shear stress by varying the channel lengths to obtain appropriate flow velocity of each channel. The CGGs and the SSGGs are fabricated and implemented using the standard soft lithography technique. The microfluidic chip consists of three layers made from PDMS. A polymer-based glucose sensor is also integrated with each chip during the PDMS bonding process. The concentration gradient profiles of CGGs were measured by dye visualization. The error between the analytical profile and measured concentration profile is less than 5%. The generated concentration profiles were independent of the flow rate within the range of 1.8 to 18 µL/min. The performance of SSGGs is determined by measuring the marching velocity of a capillary meniscus for each microchannel. The shear stress on the sidewall of a channel can be evaluated by the associated velocity. The measured results show that the proposed SSGGs produce precise shear stress gradients for the designs of the linear and logarithmic profile (R2 is higher than 0.997 for both cases). In addition, the discrepancy between the measurement results and analytical profiles is approximately 1%–5%. | en |
| dc.description.provenance | Submitted by admin ntu (admin@lib.ntu.edu.tw) on 2023-09-22T17:04:47Z No. of bitstreams: 0 | en |
| dc.description.provenance | Made available in DSpace on 2023-09-22T17:04:47Z (GMT). No. of bitstreams: 0 | en |
| dc.description.tableofcontents | 致謝 i
摘要 iii Abstract v 目錄 vii 圖目錄 xi 表目錄 xvii 符號說明 xix 第一章 緒論 1 1.1 前言 1 1.2 文獻回顧 2 1.2.1 濃度梯度產生器 2 1.2.2 剪應力梯度產生器 12 1.3 研究動機與目的 19 1.4 論文架構 20 第二章 研究理論基礎與元件設計 23 2.1 微流道設計流程與數學模型推導 23 2.2 演算法之最佳化與運算結果 27 2.2.1 入口流量比之最佳化 28 2.2.2 混合流道長度之最佳化 28 2.2.3 演算法運算結果 29 2.3 濃度梯度產生器之設計參數 31 2.4 濃度梯度產生器整合元件之設計參數 34 2.5 剪應力梯度產生器之理論 37 2.6 剪應力梯度產生器之設計參數 39 第三章 計算流體力學之模擬結果與討論 45 3.1 模擬軟體環境架設 45 3.2 不同流量下之濃度分布 46 3.2.1 五個出口高斯濃度梯度產生器模擬結果 46 3.2.2 七個出口高斯濃度梯度產生器量測結果 48 3.2.3 五個出口二次對數濃度梯度產生器模擬結果 49 第四章 製造方法與步驟 51 4.1 元件製作流程 51 4.1.1 光罩設計 53 4.1.2 微影製程 56 4.1.3 翻模轉印 63 4.2 元件組裝與製程結果 65 4.2.1 剪應力梯度產生器元件組裝 65 4.2.2 與軟性感測器整合元件之組裝 67 4.2.3 製程結果 69 第五章 量測結果與討論 73 5.1 量測架設 73 5.1.1 濃度梯度產生器整合元件之量測架設 73 5.1.2 剪應力梯度產生器之量測架設 74 5.2 濃度梯度產生器整合元件之量測結果與討論 74 5.3 剪應力梯度產生器之量測結果與討論 75 5.3.1 線性剪應力梯度產生器(四個出口)整合元件之量測結果 76 5.3.2 線性剪應力梯度產生器(五個出口)之量測結果 76 5.3.3 線性剪應力梯度產生器(六個出口)之量測結果 77 5.3.4 二次對數剪應力梯度產生器(四個出口)之量測結果 78 第六章 結論與未來展望 79 6.1 結論 79 6.2 未來展望 80 參考文獻 81 附錄A 91 | - |
| dc.language.iso | zh_TW | - |
| dc.subject | 微流體 | zh_TW |
| dc.subject | 單步稀釋 | zh_TW |
| dc.subject | 濃度梯度產生器整合 | zh_TW |
| dc.subject | 剪應力梯度產生器整合 | zh_TW |
| dc.subject | Shear stress gradient generator | en |
| dc.subject | Concentration gradient generator | en |
| dc.subject | One-step dilution | en |
| dc.subject | Microfluidics | en |
| dc.title | 微流體晶片與軟性感測晶片之整合製程及剪應力梯度晶片的開發 | zh_TW |
| dc.title | Integration of Microfluidic Chips with Polymer-Based Sensing Devices and Development of Shear Stress Gradient Generators | en |
| dc.type | Thesis | - |
| dc.date.schoolyear | 111-2 | - |
| dc.description.degree | 碩士 | - |
| dc.contributor.oralexamcommittee | 陳國聲;蘇裕軒 | zh_TW |
| dc.contributor.oralexamcommittee | Kuo-Shen Chen;Yu-Hsuan Su | en |
| dc.subject.keyword | 濃度梯度產生器整合,剪應力梯度產生器整合,單步稀釋,微流體, | zh_TW |
| dc.subject.keyword | Concentration gradient generator,Shear stress gradient generator,One-step dilution,Microfluidics, | en |
| dc.relation.page | 96 | - |
| dc.identifier.doi | 10.6342/NTU202303732 | - |
| dc.rights.note | 未授權 | - |
| dc.date.accepted | 2023-08-11 | - |
| dc.contributor.author-college | 工學院 | - |
| dc.contributor.author-dept | 機械工程學系 | - |
| 顯示於系所單位： | 機械工程學系 | |
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